Perfusion of the rat ovary: Application of pulsed arterial spin labeling MRI

Tempel, Catherine; Neeman, Michal

doi:10.1002/(sici)1522-2594(199901)41:1<113::aid-mrm16>3.0.co;2-g

Cited by 16 publications

(11 citation statements)

References 38 publications

(45 reference statements)

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“…ASL MRI, using different labeling approaches, has been applied previously for the study of perfusion in a number of organs (11–20). However, this is the first application for monitoring the directional dependent contribution of the dual arterial supply of maternal blood to the perfusion of each individual placenta.…”

Section: Discussionmentioning

confidence: 99%

“…This image pair was repeated (usually 6–8 per mouse) as necessary in order to scan all fetuses and their placentas. Presaturation of the MRI signal of blood flowing into the uterine arteries was performed using a steady‐state saturation scheme, as described by Tempel et al (20). A thick axial slice (8 mm), covering either the entire heart (denoted as upper) or the bifurcation point of the common iliac artery (denoted as lower), was saturated by a 90° hermit pulse (2 ms) followed by a crusher gradient.…”

Section: Methodsmentioning

confidence: 99%

“…ASL (11, 12), either by inversion or by saturation of water in the arterial blood, has been applied previously for the study of perfusion in a number of organs, particularly in the brain (13–15) and the kidney (16–18). ASL based on steady‐state, slice‐selective pulsed saturation similar to that applied here, was reported previously by us, for measurement of perfusion in the rat ovary (19, 20).…”

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confidence: 96%

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Unique in utero identification of fetuses in multifetal mouse pregnancies by placental bidirectional arterial spin labeling MRI

Avni

Raz

Biton

et al. 2011

Magnetic Resonance in Med

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Noninvasive imaging is a critical part of the study of developing embryos/fetuses, particularly in the context of alterations of gene expression in genetically modified animals. However, in litter-bearing animals, such as mice, the inability to accurately identify individual embryo/fetus in utero is a major obstacle to longitudinal, noninvasive in vivo studies. Arterial spin labeling MRI was adopted here to determine the fetal order along the uterine horns in vivo, based on the specific pattern of dual arterial blood supply within the mouse uterine horns. Blood enters the mouse uterus cranially through the ovarian artery and caudally through the uterine artery. Saturation slices were alternately placed on the maternal heart or on the bifurcation point of the common iliac artery, thereby saturating either downward inflow via the ovarian arteries or upward inflow via the uterine arteries, respectively. Saturation maps provided a unique signature with highly significant correlation between the direction-dependent magnetization transfer and the position of the fetuses/placentas along the uterine horns. The bidirectional arterial spin labeling-MRI method reported here opens possibilities to determine and pursue phenotypic alterations in fetuses and placentas in longitudinal studies of transgenic and knockout mice models, and for studying defects in placental vascular architecture. Magn Reson Med 68:560-570,

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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confidence: 96%

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Unique in utero identification of fetuses in multifetal mouse pregnancies by placental bidirectional arterial spin labeling MRI

Avni

Raz

Biton

et al. 2011

Magnetic Resonance in Med

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“…To minimize the risk of quantitation bias, we prefer to apply, when possible, whole body radio-frequency excitation and let blood magnetization recover between scans. (33,45,58,78,84), lungs (42,43), ovaries (67,68), and testis (53).…”

Section: Principle Of Perfusion Measurement With Aslmentioning

confidence: 99%

In vivo functional NMR imaging of resistance artery control

Carlier

Bertoldi

2005

American Journal of Physiology-Heart and Circulatory Physiology

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Arterial spin labeling (ASL) in combination with NMR imaging is an in vivo technique that quantifies tissue perfusion in absolute values (ml blood ⅐ min Ϫ1 ⅐ g tissue Ϫ1 ) with high temporal (1-10 s) and spatial (0.1-3 mm) resolution. It uses the arterial water spins as endogenous freely diffusible markers of perfusion and, hence, is a totally noninvasive method. The technique has been successfully applied to quantify baseline perfusion in many organs, including the heart, in humans and animals, and results were validated by comparison with gold standards, PET and microspheres, respectively. Because of the high sampling rate of perfusion with ASL and the possibility that measurements could be obtained without harm over indefinite periods of time, the technique has the potential for use in functional investigations of microcirculation regulation and resistance artery control in vivo. We describe examples of the use of ASL to this end. With use of specific technological developments, ASL determination of perfusion can be coupled with simultaneous acquisitions of 1 H and 31 P NMR spectroscopy data. These protocols offer new possibilities whereby the microcirculatory control of cell oxygenation and high-energy phosphate metabolism can be explored.arterial spin labeling; imaging; microcirculation; perfusion AT FIRST SIGHT, NONINVASIVE in situ visualization of resistance artery lumen and wall is well beyond the technological limits of in vivo NMR imaging, which has detection limits of 0

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“…In rats, NMR microscopic examination of large antral follicles excised from ovaries was reported by Tempel et al [13,14]. They also examined perfusion in rat ovaries by spin labeling in vivo [14,15] using immature rats treated with PMSG and hCG to induce follicle maturation. They characterized antral follicles as spherical structures with a perfused periphery and an unperfused antrum.…”

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Comparison of Nuclear Magnetic Resonance Microimages and Histology of Rat Ovaries.

Sugimoto

Manabe

Morita

et al. 2000

Journal of Reproduction and Development

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Abstract. Nuclear magnetic resonance (NMR) microscopy is a magnetic resonance imaging technique with an enhanced spatial resolution. In this study, fresh ovaries of mature rats immersed in phosphate buffered saline (PBS) were examined by NMR microscopy. Histological sections corresponding to NMR microimages were prepared, and components in ovaries were identified in microimages by comparison with light microscopic images. Signal intensities of antral follicles, corpora lutea (CL), stroma, adjacent fat tissue and PBS were measured in images with different echo times (TE) and analyzed. As TE increased, signal intensities decreased in CL and connective tissue including the stroma, while those in PBS and follicles changed less. Fat showed significantly high signals compared with other components at each TE. Stroma showed significantly low signals than other components except PBS and follicles at TE of 5 msec. There were no significant differences among CL, follicles and PBS at TE of 5 msec. At TE of 20 msec, however, there were significant differences in signal intensities between each components in the rank order fat > PBS > follicles > CL > stroma. Significant regression of signal intensity was seen on TE in follicles, CL and stroma. NMR microscopy is considered to be a useful tool to investigate the physiological function of rat ovaries.

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Perfusion of the rat ovary: Application of pulsed arterial spin labeling MRI

Cited by 16 publications

References 38 publications

Unique in utero identification of fetuses in multifetal mouse pregnancies by placental bidirectional arterial spin labeling MRI

Unique in utero identification of fetuses in multifetal mouse pregnancies by placental bidirectional arterial spin labeling MRI

In vivo functional NMR imaging of resistance artery control

Comparison of Nuclear Magnetic Resonance Microimages and Histology of Rat Ovaries.

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