Perfusion fixation and alcian‐blue PAS staining in histology and histopathology of teleost kidney

Hentschel, H.; Finkenstadt, H.

doi:10.1111/j.1365-2761.1980.tb00434.x

Cited by 5 publications

(3 citation statements)

References 9 publications

(13 reference statements)

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“…In addition to structural features described in previous studies (Hentschel & Finkenstadt 1980), semi-thin sections from tissue samples of all regions of the opisthonephric kidney revealed the following features for diseased trout.…”

Section: Light Microscopysupporting

confidence: 52%

“…In addition to clinical signs which are generally considered to be characteristic of VHS (Einkenstadt 1979), histological observations of the opisthonephric kidney from diseased cultured rainbow trout, Salnw gairdneri Richardson, revealed marked alterations of the renal corpuscles (Hentschel & Finkenstadt 1980). Most of the glomeruli exhibited a thicken ing of the basement membrane which stained strongly with the periodic acid-Schiff's technique, indicating changes comparable with membranoproliferative glomerulonephritis of mammals (Meadows 1978).…”

Section: Introductionmentioning

confidence: 99%

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Glomerular disease in cultured rainbow trout, Salmo gairdneri Richardson, suffering from presumptive chronic viral haemorrhagic septicaemia

Elger

Hentschel

1983

Journal of Fish Diseases

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Severe glomerular changes in the kidney of rainbow trout suffering from presumptive chronic viral haemorrhagic septicaemia are described. The pathological changes, as revealed by light-and electron microscopy, resembled those of membranous glomerulonephritis in mammals. Large electron-dense subendothelialmesangial deposits were present in most glomeruli. A lattice-like arrangement of macromolecules was observed. Monocytes and macrophages were observed in the capillary convolute. The possibility of post-infectious disease nephropathy with aggregation of immunocomplexes in the capillary wall in fishes is discussed.

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Section: Light Microscopysupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Glomerular disease in cultured rainbow trout, Salmo gairdneri Richardson, suffering from presumptive chronic viral haemorrhagic septicaemia

Elger

Hentschel

1983

Journal of Fish Diseases

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“…Segmentation of the rainbow trout nephron has been shown by Hentschel & Finkenstadt (1980) as well as Yasutake &Wales (1983), and terminology of tubular segments is based on the review by Hentschel & Elger (1987). Since there is appreciable heterogene~ty in nephrons due to age and environmental condit~ons (Hentschel & Elger 1987), an outline of the ultrastructure of control kidneys is given.…”

Section: Controlsmentioning

confidence: 99%

Cytopathology of liver and kidney in rainbow trout Oncorhynchus mykiss after long-term exposure to sublethal concentrations of linuron

Oulmi¹,

Negele²,

Braunbeck³

1995

Dis. Aquat. Org.

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Hepatic and renal cytopathological alterations in fingerl~ng rainbow trout Oncorhynchus mykiss following 5 \vk exposure to 30, 120, and 240 pg I -' linuron [3(3,4-dichloropheny1)-l-methoxy-lmethylurea] were studied by electron microscopy. Ultrastructural alterations were detected in liver and kidney at 230 pg l.', 2 orders of magnitude below conventional LCo. The response suggested a doseresponse relationship with a change from adaptive to degenerative features at 120 pg I-' Hepatocyte changes included: stimulation of mitosis; segmentation of nuclei; partial reorganization of heterochromatin; multiplication of nucleoli; fractionation, vesiculation and transformation of rough endoplasmic reticulum (RER) into myelinated bodies; induction of smooth endoplasmic reticulum; moderate steatosis; apparent proliferation of mitochondria, peroxlsomes, Golgi fields and lysosomal elements; depletion of glycogen; perisinusoidal lipid accumulat~on; elevated rate of hepatocytes in various stages of necrosis; infiltration and increased phagocytic activity of macrophages. Reactions of renal tubular cells were differentiated in different nephron segments. Major alterations by site in kidney were (1) renal corpuscle: lobulation of podocyte nuclei; (2) proximal segment I: elevated heterogeneity of all cell components, increased heterochromatin and nuclear size, rearrangement of RER, proliferation of Golgi fields, novel lysosomal elements, decreased mitochondria and lysosomes at 240 1-19 I-'; (3) proximal segment 11: nuclear lobulation, binucleated cells, proliferation of lysosomes and peroxisomes (lower concentrations), decreased peroxisomes and mitochondria (240 pg I-'), crystalline inclusions in lysosomal matrix, fragmentation, degranulation and circular arrangement of RER; (4) distal segment: induction of giant mitochondria with longitudinal crystalline inclusions, atypical lysosomes with long crystalline matrix inclusions, and augmentation of various lysosomal elements. Comparison of linuroninduced cellular alterations with cytopathological effects by potential linuron breakdown products, namely 4-chloroaniline and 3,4-dichloroaniline, revealed a high degree of s~milarity of cytopathological phenomena, indicating that part of the changes observed after linuron exposure might well be due to the action of linuron metabolites.

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Cell junctions in the renal tubule of a fresh-water teleost, Salmo gairdneri Rich.

Elger

Hentschel

1986

Cell Tissue Res.

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Perfusion fixation and alcian‐blue PAS staining in histology and histopathology of teleost kidney

Abstract: Perfusion fixation of trout kidney with picric-acid: formaldehyde: acetic acid: ethanol mixture was compared with other methods as a fixative for staining with the alcian-blue PAS technique. Perfusion fixation gave conspicuously better results for light microscopical purposes.

Cited by 5 publications

References 9 publications

Glomerular disease in cultured rainbow trout, Salmo gairdneri Richardson, suffering from presumptive chronic viral haemorrhagic septicaemia

Glomerular disease in cultured rainbow trout, Salmo gairdneri Richardson, suffering from presumptive chronic viral haemorrhagic septicaemia

Cytopathology of liver and kidney in rainbow trout Oncorhynchus mykiss after long-term exposure to sublethal concentrations of linuron

Cell junctions in the renal tubule of a fresh-water teleost, Salmo gairdneri Rich.

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