Perfusion-decellularized skeletal muscle as a three-dimensional scaffold with a vascular network template

Zhang, Jian; Hu, Zhiyan; Turner, Neill J.; Teng, Shi Feng; Cheng, Wen Yue; Zhou, Hai Yang; Zhang, Li; Hu, Hong; Wang, Qiang; Badylak, Stephen F.

doi:10.1016/j.biomaterials.2016.02.040

Cited by 124 publications

(89 citation statements)

References 48 publications

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“…2E) which, in the skeletal muscle, have been shown to improve acellular matrices engraftment with the host vasculature in vivo [59]. In fact, perfused scaffolds facilitate in vivo construct anastomosis and colonization by host cells and, thus, several strategies have been emerging for the development of vasculature conduits in tissue engineering applications [59][60][61][62].…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Three-dimensional scaffolds of fetal decellularized hearts exhibit enhanced potential to support cardiac cells in comparison to the adult

et al. 2016

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Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Three-dimensional scaffolds of fetal decellularized hearts exhibit enhanced potential to support cardiac cells in comparison to the adult

et al. 2016

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“…In addition, the amount of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme, and smooth muscle actin decreased with the SDS/SD endonuclease treatment. Another multistep protocol utilized trypsin/ethylenediaminetetraacetic acid (EDTA), SDS, Triton X-100, peracetic acid/ethanol, and DNase to decellularize porcine rectus abdominis muscle [70]. Cells and DNA were sufficiently removed and laminin, collagen type IV, and fibronectin remained following treatment.…”

Section: Decellularization Strategiesmentioning

confidence: 99%

Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications

Gilpin

Yang

2017

BioMed Research International

565

578

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As the gap between donors and patients in need of an organ transplant continues to widen, research in regenerative medicine seeks to provide alternative strategies for treatment. One of the most promising techniques for tissue and organ regeneration is decellularization, in which the extracellular matrix (ECM) is isolated from its native cells and genetic material in order to produce a natural scaffold. The ECM, which ideally retains its inherent structural, biochemical, and biomechanical cues, can then be recellularized to produce a functional tissue or organ. While decellularization can be accomplished using chemical and enzymatic, physical, or combinative methods, each strategy has both benefits and drawbacks. The focus of this review is to compare the advantages and disadvantages of these methods in terms of their ability to retain desired ECM characteristics for particular tissues and organs. Additionally, a few applications of constructs engineered using decellularized cell sheets, tissues, and whole organs are discussed.

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“…The poor clinical outcome following VML injury combined with a lack of effective treatment options motivates our exploration of VML regeneration. To better understand the sequence of events that follow VML injury and aid in the development of effective treatment strategies, various pre-clinical animal models have been developed (Chen and Walters 2013; Hou and others 2016; Turner and others 2012; Wu and others 2012; Zhang and others 2016). Among these, the hind limb muscles of the rat are a reliable pre-clinical model that recapitulates healing with non-contractile scar tissue observed in humans following VML injury (Wu and others 2012).…”

Section: Introductionmentioning

confidence: 99%

Recovery from volumetric muscle loss injury: A comparison between young and aged rats

Kim

Kasukonis

Brown

et al. 2016

Experimental Gerontology

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Termed volumetric muscle loss (VML), the bulk loss of skeletal muscle tissue either through trauma or surgery overwhelms the capacity for repair, leading to the formation of non-contractile scar tissue. The myogenic potential, along with other factors that influence wound repair are known to decline with age. In order to develop effective treatment strategies for VML injuries that are effective across a broad range of patient populations, it is necessary to understand how the response to VML injury is affected by aging. Towards this end, this study was conducted to compare the response of young and aged animal groups to a lower extremity VML injury. Young (3 months, n=12) and aged (18 months, n=8) male Fischer 344 rats underwent surgical VML injury of the tibialis anterior muscle. Three months after VML injury it was found that young TA muscle was on average 16% heavier than aged muscle when no VML injury was performed and 25% heavier when comparing VML treated young and aged animals (p<0.0001, p<0.0001). Peak contractile force for both the young and aged groups was found to decrease significantly following VML injury, producing 65% and 59% of the contralateral limbs’ peak force, respectively (p<0.0001). However, there were no differences found for peak contractile force based on age, suggesting that VML affects muscle’s ability to repair, regardless of age. In this study, we used the ratio of collagen I to MyoD expression as a metric for fibrosis vs. myogenesis. Decreasing fiber cross-sectional area with advancing age (p<0.005) coupled with the ratio of collagen I to MyoD expression, which increased with age, supports the thought that regeneration is impaired in the aged population in favor of fibrosis (p=0.0241). This impairment is also exacerbated by the contribution of VML injury, where a 77-fold increase in the ratio of collagen I to MyoD was observed in the aged group (p<0.0002). The aged animal model described in this study provides a tool for investigators exploring not only the development of VML injury strategies but also the effect of aging on muscle regeneration.

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Perfusion-decellularized skeletal muscle as a three-dimensional scaffold with a vascular network template

Cited by 124 publications

References 48 publications

Three-dimensional scaffolds of fetal decellularized hearts exhibit enhanced potential to support cardiac cells in comparison to the adult

Three-dimensional scaffolds of fetal decellularized hearts exhibit enhanced potential to support cardiac cells in comparison to the adult

Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications

Recovery from volumetric muscle loss injury: A comparison between young and aged rats

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