Perfused rat intrahepatic bile ducts secrete and absorb water, solute, and ions

Masyuk, Anatoly I.; Gong, Ai Yu; Kip, Sertac N.; Burke, Michael J.; LaRusso, Nicholas F.

doi:10.1053/gast.2000.20248

Cited by 46 publications

(52 citation statements)

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“…Forskolin induced a sustained increase in luminal pH (n=3; Figure 1b), similar to what has been reported previously (12). This response was similar to the pattern observed during stimulation with secretin (100 nM; data not shown), which activates G s and then adenylyl cyclase via the secretin receptor (3).…”

Section: Comparison Of Cyclic Amp-mediated and Ca 2+ -Mediated Bicarbsupporting

confidence: 88%

“…Intrahepatic bile duct units (IBDUs) were microdissected from normal rat liver as described previously (12). Briefly, rats were anesthetized with pentobarbital sodium (50mg/kg ip), then the portal vein was exposed and cannulated and the liver was perfused with ice-cold saline.…”

Section: Isolation Of Intrahepatic Bile Duct Segmentsmentioning

confidence: 99%

“…Confocal images were obtained at a rate of 5-20 images/min. In situ calibration curves were generated at the end of each experiment by perfusion with 10 μM nigericin plus monensin in serial solutions of pH 6.8, 7.4, and then 8.0, as described (12). Non-ratio images of BCECF-dextran were collected to determine luminal pH, and potential errors from such measurements include photobleaching and changes in the volume of distribution of the dye.…”

Section: Biliary Ph Measurementsmentioning

confidence: 99%

“…Intrahepatic segments of bile ducts were microdissected from rat liver as described previously (12), then perfused with the cell-impermeant pH dye BCECF-dextran while examined by confocal microscopy in order to monitor bicarbonate secretion into the lumen (Figure 1a). In selected experiments, IBDU were maintained in culture overnight prior to microperfusion, which enabled IBDU to be treated with siRNA constructs prior to study.…”

Section: Measurement Of Bicarbonate Secretion In Microdissected Micrmentioning

confidence: 99%

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Cyclic AMP Regulates Bicarbonate Secretion in Cholangiocytes Through Release of ATP Into Bile

et al. 2007

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Section: Comparison Of Cyclic Amp-mediated and Ca 2+ -Mediated Bicarbsupporting

confidence: 88%

Section: Isolation Of Intrahepatic Bile Duct Segmentsmentioning

confidence: 99%

Section: Biliary Ph Measurementsmentioning

confidence: 99%

Section: Measurement Of Bicarbonate Secretion In Microdissected Micrmentioning

confidence: 99%

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Cyclic AMP Regulates Bicarbonate Secretion in Cholangiocytes Through Release of ATP Into Bile

et al. 2007

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“…The mechanosensory function of cholangiocyte cilia was addressed directly by using a microperfused rat intrahepatic bile duct unit model (IBDU) that allows controlled manipulation of luminal fluid flow rates, detection of both [Ca 2+ ] i and cAMP levels, and effective utilization of gene silencing by siRNAs. [22][23][24] …”

Section: Introductionmentioning

confidence: 99%

Cholangiocyte Cilia Detect Changes in Luminal Fluid Flow and Transmit Them Into Intracellular Ca2+ and cAMP Signaling

et al. 2006

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Background & Aims-Each cholangiocte in the biliary tree has a primary cilium extending from the apical plasma membrane into the ductal lumen. While the physiological significance of cholangiocyte cilia is unknown, studies in renal epithelia suggest that primary cilia possess sensory functions. Here, we tested the hypothesis that cholangiocyte cilia are sensory organelles that detect and transmit luminal bile flow stimuli into intracellular Ca 2+ ([Ca 2+ ] i ) and cAMP signaling.

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Basosquamous carcinoma

Leibovitch

Huilgol

Selva

et al. 2005

Cancer

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BACKGROUNDBasosquamous carcinoma (BSC) is a rare tumor defined as a basal cell carcinoma (BCC) differentiating into squamous cell carcinoma (SCC). It is reported to have a high rate of recurrence with standard wide local excision. The aim of the current study was to report a large series of patients with BSC treated with Mohs micrographic surgery (MMS).METHODSThe prospective, multicenter case series included all patients in Australia treated with MMS for BSC, who were monitored by the Skin and Cancer Foundation Australia between 1993 and 2002.RESULTSMost of the 178 tumors (95.6%) were located in the head and neck area. Recurrent tumors occurred in 47.8% of patients. The tumors were diagnosed initially as BCC in 87.4% and as SCC in 12.0% of patients. Perineural invasion was recorded in 7.9% of patients with data available. Most of these (69.0%) were previously recurrent tumors. Of 98 patients who completed a 5‐year follow‐up period after MMS, 4 (4.1%) had disease recurrence.CONCLUSIONSThe low 5‐year disease recurrence rate of BSC with MMS emphasized the importance of margin‐controlled excision using MMS. Cancer 2005. © 2005 American Cancer Society.

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Perfused rat intrahepatic bile ducts secrete and absorb water, solute, and ions

Cited by 46 publications

References 20 publications

Cyclic AMP Regulates Bicarbonate Secretion in Cholangiocytes Through Release of ATP Into Bile

Cyclic AMP Regulates Bicarbonate Secretion in Cholangiocytes Through Release of ATP Into Bile

Cholangiocyte Cilia Detect Changes in Luminal Fluid Flow and Transmit Them Into Intracellular Ca2+ and cAMP Signaling

Basosquamous carcinoma

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