Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability

Wayman, Christina; Duricki, Denise; Roy, Lisa A; Haenzi, Barbara; Tsai, Shi-Yen; Kartje, Gwendolyn L.; Beech, John S.; Cash, Diana; Moon, Lawrence D. F.

doi:10.3791/53106-v

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…In such studies, MCAO is one of the most commonly employed surgical procedures to induce ischemic stroke (27). This model, known as the permanent MCAO model, has been developed to mimic human ischemic stroke, and generates cerebral infarction in both cortical and subcortical areas (28). The present study used the MCAO model to investigate 6-gingerol as an experimental therapy.…”

Section: Discussionmentioning

confidence: 99%

Ameliorative effects of 6‑gingerol in cerebral ischemia are mediated via the activation of antioxidant and anti‑inflammatory pathways

Kongsui

Jittiwat

2023

Biomed Rep

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Focal ischemia occurs when an embolus or thrombus occludes an artery, causing the rapid obstruction of cerebral blood flow. Although stroke represents a main cause of disability and mortality in developing countries, therapeutic approaches available for this condition remain very limited. The aim of the present study was to examine the effects of the phytochemical, 6-gingerol, on the brain infarct volume, neuronal loss and on the oxidative stress parameters, cyclooxygenase-2 (COX-2) and interleukin (IL)-6, in an animal model of focal ischemic stroke. Male Wistar rats, weighing 250-300 g, were divided into the following six groups: i) The control; ii) right middle cerebral artery occlusion (Rt.MCAO) + vehicle; iii) Rt.MCAO + piracetam; iv) Rt.MCAO + 6-gingerol (6-Gin) at 5 mg/kg body weight (BW); v) Rt.MCAO + 6-Gin at 10 mg/kg BW; and vi) the Rt.MCAO + 6-Gin at 20 mg/kg BW group. The rats in each group received the vehicle or piracetam or 6-gingerol intraperitoneally for 7 days following Rt.MCAO. The brain infarct volume, neuronal loss and alterations in antioxidant and anti-inflammatory levels were assessed in the cortex and hippocampus. The results revealed that the brain infarct volume, malondialdehyde level and the density ratio of COX-2 and IL-6 to β-actin were significantly decreased following treatment with 6-gingerol. In addition, neuronal density and superoxide dismutase activity in the cortex and hippocampus were increased. On the whole, the findings of the present study suggest that 6-gingerol exerts antioxidant and anti-inflammatory effects in vivo, which effectively ameliorate the brain damage induced by focal cerebral ischemic strok

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Section: Discussionmentioning

confidence: 99%

Ameliorative effects of 6‑gingerol in cerebral ischemia are mediated via the activation of antioxidant and anti‑inflammatory pathways

Kongsui

Jittiwat

2023

Biomed Rep

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“…A vertical incision was made on the neck, and the left CCA was carefully dissected from the surrounding tissue and exposed, avoiding damaging the vagus nerve. 23,30 A 3-0 surgical silk thread (Ethicon, France) was placed around the CCA and remained untightened until occlusion during the imaging session. Rats from MCAo and CCAo + MCAo groups were subjected to a permanent MCA occlusion directly during the imaging session using a microvascular clip (#18055-03, micro-serrefine, FST GmbH, Germany) that was manually refined by grinding for optimal serration of the MCAo in adult rats.…”

Section: Methodsmentioning

confidence: 99%

Brain-wide continuous functional ultrasound imaging for real-time monitoring of hemodynamics during ischemic stroke

Brunner

Denis

Gertz

et al. 2023

J Cereb Blood Flow Metab

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Ischemic stroke occurs abruptly causing sudden neurologic deficits, and therefore, very little is known about hemodynamic perturbations in the brain immediately after stroke onset. Here, functional ultrasound imaging was used to monitor variations in relative cerebral blood volume (rCBV) compared to baseline. rCBV levels were analyzed brain-wide and continuously at high spatiotemporal resolution (100 μm, 2 Hz) until 70mins after stroke onset in rats. We compared two stroke models, with either a permanent occlusion of the middle cerebral artery (MCAo) or a tandem occlusion of both the common carotid and middle cerebral arteries (CCAo + MCAo). We observed a typical hemodynamic pattern, including a quick drop of the rCBV after MCAo, followed by spontaneous reperfusion of several brain regions located in the vicinity of the ischemic core. The severity and location of the ischemia were variable within groups. On average, the severity of the ischemia was in good agreement with the lesion volume (24 hrs after stroke) for MCAo group, while larger for the CCAo + MCAo model. For both groups, we observed that infarcts extended to initially non-ischemic regions located rostrally to the ischemic core. These regions strongly colocalize with the origin of transient hemodynamic events associated with spreading depolarizations.

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MRI characterization of rat brain aging at structural and functional level: Clues for translational applications

Bontempi

Podda

Daducci

et al. 2021

Experimental Gerontology

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Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability

Cited by 3 publications

References 8 publications

Ameliorative effects of 6‑gingerol in cerebral ischemia are mediated via the activation of antioxidant and anti‑inflammatory pathways

Ameliorative effects of 6‑gingerol in cerebral ischemia are mediated via the activation of antioxidant and anti‑inflammatory pathways

Brain-wide continuous functional ultrasound imaging for real-time monitoring of hemodynamics during ischemic stroke

MRI characterization of rat brain aging at structural and functional level: Clues for translational applications

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