Performing ICSI within 4 hours after denudation optimizes clinical outcomes in ICSI cycles

Yini, Zhang; Ma, Yongzhuang; Fang, Zongjuan; Hu, Shengqing; Li, Zhou; Zhu, Lixia; Jin, Lei

doi:10.1186/s12958-020-00587-y

Cited by 9 publications

(17 citation statements)

References 34 publications

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“…Seventeen studies reported data on the effect of OPU-DN on fertilisation rate. Among them, 11 reported no significant effect of OPU-DN time on fertilisation rate, which was similar to the conclusion of a recent study, suggesting no correlation between OPU-DN time and fertilisation rate [ 33 ]. However, six studies considered that OPU-DN time did affect fertilisation rate, and five of these believed that the fertilisation rate would increase with the extension of OPU-DN time [ 2 , 4 , 15 , 17 , 23 ].…”

Section: Resultssupporting

confidence: 84%

“…Another study showed that the fertilisation rate was stable within 6 h after degranulation, and the stability decreased remarkably after 6 h [ 3 ], while two others suggested that prolonging the time of DN-ICSI could increase the fertilisation rate [ 4 , 20 ]. However, one recent study that grouped subjects according to DN-ICSI time found that, although the fertilisation rate gradually increased with incubation times up to 5 h, it decreased considerably after 5 h [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

“…Four of the five studies revealed that there was no effect of DN-ICSI time on embryo development. One study showed a non-significant decreasing trend in the frequency of good-quality embryos when the DN-ICSI was more than 5 h [ 33 ]. However, another study found more good-quality embryos in a group incubated for 3 h after DN compared to a group with immediate ICSI (37.3% vs. 27.9%; p < 0.05) [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

“…Another study found that the results varied with different cut-off values of DN-ICSI time. When the cut-off value was 2 h, the implantation rate was strikingly higher in the < 2-h group than in the > 2-h group, but there was no significant difference between the two groups when the cut-off value was 3 or 4 h [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

“…In contrast, one study suggested that longer DN-ICSI duration lowered the clinical pregnancy rate, which found that each 1-h increase in DN-ICSI time reduced the likelihood of clinical pregnancy by 7.9% [ 14 ]. Recently, Zhang et al [ 33 ] reported that the clinical pregnancy rate did not vary with DN-ICSI times less than 4 h, but did decrease notably over 4 h. There was no statistical difference in the four studies that analysed the effect of DN-ICSI time on live birth rate [ 17 , 19 , 20 , 24 ]. The results and conclusions are listed in Table 4 .…”

Section: Resultsmentioning

confidence: 99%

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Effect of the time interval between oocyte retrieval and ICSI on embryo development and reproductive outcomes: a systematic review

Xiao

Sun

Zhen

et al. 2021

Reprod Biol Endocrinol

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Background Intra-cytoplasmic sperm injection (ICSI) is used in assisted reproductive technology (ART) laboratories. However, there is no consensus regarding the precise time intervals within ICSI cycles [oocyte pick up (OPU), oocyte denudation (DN), and ICSI], and results are inconsistent and contradictory. Thus, we aim to evaluate whether there is a concordance regarding the time intervals used in different laboratories and a concrete time that gives better laboratory and reproductive results. Methods A systematic review of the literature until July 25, 2020, was performed with the keywords “Oocyte Denudation/Denudation/Oocyte,” “Intra-cytoplasmic Sperm Injection/ICSI,” “Oocyte/Oocyte maturation/ cumulus,” and “Cumulus removal/ removal.” Articles and abstracts in English and involving human subjects referring to the effects of oocyte DN time on embryo development and clinical outcomes were included. Results Of the 294 evaluated articles, 24 (including 20 full articles and 4 abstracts) were included in this review. Eighteen studies analysed the effect of OPU-DN time on embryo development and clinical outcomes. Most of these studies concluded that OPU-DN time did not influence ICSI outcomes, but some suggested that oocytes should be incubated for a short time before DN to improve oocyte maturity and enhance ICSI outcomes. In addition to reports on positive or negligible effects, adverse effects were reported in 12 studies on DN-ICSI timing. Neither OPU-DN nor DN-ICSI time could improve live birth rate. Conclusions Oocytes should be pre-incubated for a short duration (preferably < 4 h) before DN according to the ART laboratory schedule. More randomised controlled trials are warranted to clarify the effect of DN-ICSI timing on ICSI outcomes.

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Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Effect of the time interval between oocyte retrieval and ICSI on embryo development and reproductive outcomes: a systematic review

Xiao

Sun

Zhen

et al. 2021

Reprod Biol Endocrinol

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In vivo and in vitro postovulatory aging: when time works against oocyte quality?

Nisio

Antonouli

Damdimopoulou

et al. 2022

J Assist Reprod Genet

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In mammalian species an optimal fertilization window during which successful fertilization occurs. In the majority of mammals estrus marks ovulation time and coincident with mating, thereby allowing the synchronized meeting in the fallopian tubes, between freshly ejaculated sperm and freshly ovulated oocytes. Conversely, women do not show natural visual signs of ovulation such that fertilization can occur hours later involving an aged oocyte and freshly ejaculated spermatozoa. During this time, the oocyte undergoes a rapid degradation known as “postovulatory aging” (POA). POA may become particularly important in the human-assisted reproductive technologies, as the fertilization of retrieved mature oocytes can be delayed due to increased laboratory workload or because of unforeseeable circumstances, like the delayed availability of semen samples. This paper is an updated review of the consequences of POA, either in vivo or in vitro, on oocyte quality with particular attention to modifications caused by POA on oocyte nuclear, cytoplasmic, genomic, and epigenetic maturation, and embryo development.

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Aggressive sperm immobilization improves reproductive outcomes in patients with suboptimal semen parameters and previous ICSI fertilization failure

Chou,

Chen,

Chang

et al. 2024

Sci Rep

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Intracytoplasmic sperm injection (ICSI) is the most effective procedure to resolve male infertility, enhancing overall fertilization and pregnancy outcomes. However, it is important to note that fertilization failure (FF) can still occur in a few cases after ICSI. This study aims to introduce a specialized technique of aggressive sperm immobilization for ICSI and evaluate its impact on reproductive outcomes in cases involving prior fertilization failure. All infertile couples with male partners having suboptimal semen samples and previous ICSI fertilization failure were evaluated using retrospective data from National Taiwan tertiary university hospital (NTUH) between January 2016 and February 2022. Fertilization failure in our study was defined as less than 30% fertilization rate (FR, the number of normally fertilized oocytes divided by the total number of injected mature oocytes). Data involving both standard (routine procedure) and aggressive sperm immobilization (SI) techniques during different ICSI cycles were included in this study. Standard and aggressive SI methods were performed by compressing the distal half tail of the spermatozoa ≦ 5 and 15 times prior to ICSI respectively. Generalized estimating equations analysis were applied to compare the clinical outcomes between two procedures. Overall, data from 23 infertile couples who had undergone 65 ICSI cycles (31 standard SI with low fertilization rate and 34 aggressive SI) were included in the study. The average FR in the ICSI cycles with standard SI and aggressive SI were 23.6 ± 23.1% and 49.5 ± 31.8 respectively (P = 0.0002). The majority of embryos were transferred at the day 3 stage, with an average number transferred of 2.6 ± 0.9 in the aggressive SI group and 1.9 ± 0.9 in the standard group. The number of embryos transferred per transfer cycle was higher in the aggressive SI (P = 0.015), whereas the number of good-quality embryos was similar between the two procedures (P = 0.44). There were one and seven live births from the standard SI cycles and aggressive SI cycles respectively. In conclusion, aggressive SI was associated with a significantly higher FR, resulting in more available embryos for transfer without compromising embryo quality. Therefore, this specialized technique improved pregnancy outcome among infertile couples with a previous ICSI–FF. It can be a safe, economic, and effective method to improve the assisted reproductive technologies outcomes for infertile patients affected by previous ICSI–FF.

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Performing ICSI within 4 hours after denudation optimizes clinical outcomes in ICSI cycles

Cited by 9 publications

References 34 publications

Effect of the time interval between oocyte retrieval and ICSI on embryo development and reproductive outcomes: a systematic review

Effect of the time interval between oocyte retrieval and ICSI on embryo development and reproductive outcomes: a systematic review

In vivo and in vitro postovulatory aging: when time works against oocyte quality?

Aggressive sperm immobilization improves reproductive outcomes in patients with suboptimal semen parameters and previous ICSI fertilization failure

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