Performances of Capillary Electrophoresis and HPLC Methods in HbA<sub>1c</sub> Determination: Diagnostic Accuracy in HbS and HbD‐Iran Variants' Presence

Dessı̀, Mariarita; Pieri, Massimo; Pignalosa, Stefano; Martino, Francesca Gabriela; Zenobi, Rossella

doi:10.1002/jcla.21728

Cited by 18 publications

(11 citation statements)

References 17 publications

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“…Recently, capillary 2-FP analyzer has been found to be suitable for HbA1c measurement, and sometimes, it showed some advantages with respect to the HPLC analyzers tested, especially when Hb variants are present. 72 …”

Section: Methods For Hba1c Analysismentioning

confidence: 99%

Significance of HbA1c Test in Diagnosis and Prognosis of Diabetic Patients

et al. 2016

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Diabetes is a global endemic with rapidly increasing prevalence in both developing and developed countries. The American Diabetes Association has recommended glycated hemoglobin (HbA1c) as a possible substitute to fasting blood glucose for diagnosis of diabetes. HbA1c is an important indicator of long-term glycemic control with the ability to reflect the cumulative glycemic history of the preceding two to three months. HbA1c not only provides a reliable measure of chronic hyperglycemia but also correlates well with the risk of long-term diabetes complications. Elevated HbA1c has also been regarded as an independent risk factor for coronary heart disease and stroke in subjects with or without diabetes. The valuable information provided by a single HbA1c test has rendered it as a reliable biomarker for the diagnosis and prognosis of diabetes. This review highlights the role of HbA1c in diagnosis and prognosis of diabetes patients.

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Section: Methods For Hba1c Analysismentioning

confidence: 99%

Significance of HbA1c Test in Diagnosis and Prognosis of Diabetic Patients

et al. 2016

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“…HbA 1c is used as the gold standard for monitoring long‐term glycemic control in people with diabetes mellitus and is characterized by nonenzymatic binding of glucose to the N‐terminal valine residue on the β‐chain of the HbA . Previously, studies have shown that hemoglobin variants can affect the detection of HbA 1c . In this study, we incidentally found a variant of the α2 chain in a Chinese individual following HbA 1c analysis.…”

Section: Introductionmentioning

confidence: 68%

Hb I: A α‐globin chain variant causing unexpected HbA_1c results

Sun

et al. 2018

Clinical Laboratory Analysis

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“…C2FP is a new assay to evaluate HbA 1c based on the separation of Hb property and charge by capillary electrophoresis. There is a strong consistency between the results of C2FP and VII [19]. Many literatures have reported that the resolution of C2FP is superior to CE-HPLC resulting from allowing the separation of many common and rare Hb variants from the HbA0 fraction [19–21].…”

Section: Discussionmentioning

confidence: 88%

False measurement of glycated hemoglobin in patients without hemoglobin A

et al. 2019

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: Hemoglobin (Hb) A, a biochemical marker widely used in monitoring diabetes mellitus, can be quantitatively measured by various examining systems. However, significant errors still exist In this study, we evaluated the HbA level in five patients with compound heterozygotes by five different examining systems and our goal is to identify the existence of erroneous HbA measurement. Blood samples collected from normal (no hemoglobin variants) and abnormal (compound heterozygotes) patients were analyzed by capillary electrophoresis technique and sequence analysis. The samples without HbA expression via above methods were further analyzed for HbA by ion exchange HPLC Variant Ⅱ/ Variant ⅡTurbo 2.0 (VII and VⅡ-T 2.0), boronate affinity HPLC, capillary electrophoresis and Tinaquant immunoassay. HbA expression were unexpectedly detected in the compound heterozygous samples by using additional examining systems: The HPLC VⅡ and VⅡ-T 2.0 detected HbA expression in two of five samples and failed to detect the abnormal HbA expression; The CE system detected HbA expression in one of five sample with abnormal HbA expression; The Ultra2 and PPI system detected the HbA expression of all samples without abnormal HbA Five human samples without HbA expression were additionally detected with HbA expression with or without abnormal HbA expression by five analysis systems and the different examining assay potentially affected the test results. These results demonstrated that the limitations of current examining systems for monitoring patients with hemoglobin disorders, highlighting the further improvement in the method of clinical HbA examination.

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Performances of Capillary Electrophoresis and HPLC Methods in HbA_1c Determination: Diagnostic Accuracy in HbS and HbD‐Iran Variants' Presence

Cited by 18 publications

References 17 publications

Significance of HbA1c Test in Diagnosis and Prognosis of Diabetic Patients

Significance of HbA1c Test in Diagnosis and Prognosis of Diabetic Patients

Hb I: A α‐globin chain variant causing unexpected HbA_1c results

False measurement of glycated hemoglobin in patients without hemoglobin A

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Performances of Capillary Electrophoresis and HPLC Methods in HbA1c Determination: Diagnostic Accuracy in HbS and HbD‐Iran Variants' Presence

Cited by 18 publications

References 17 publications

Significance of HbA1c Test in Diagnosis and Prognosis of Diabetic Patients

Significance of HbA1c Test in Diagnosis and Prognosis of Diabetic Patients

Hb I: A α‐globin chain variant causing unexpected HbA1c results

False measurement of glycated hemoglobin in patients without hemoglobin A

Contact Info

Product

Resources

About

Performances of Capillary Electrophoresis and HPLC Methods in HbA_1c Determination: Diagnostic Accuracy in HbS and HbD‐Iran Variants' Presence

Hb I: A α‐globin chain variant causing unexpected HbA_1c results