No relationships reported)Dipeptidyl-Peptidase IV (DPP-IV) is an enzyme present in the blood, acting as a regulator for incretin hormones, such as glucagon-like peptide(GLP-1) and glucose-dependent insulinotropic polypeptide (GIP). As a result of this regulatory action, DPP-IV has become an enzyme of interest in the pathology of Type 2 Diabetes. Currently, values are measured primarily in the plasma using a fluorometric assay; however, the stability of DPP-IV activity during storage over several weeks is of question. PURPOSE: To determine the stability of plasma DPP-IV activity following freezer storage. METHODS: Plasma samples were collected from 7 healthy college aged students. Blood was collected using standard venipuncture from a prominent antecubital vein. Plasma was separated by centrifugation for 10 minutes at 1,000g and 4°C. It was then removed and stored in 0.5mL aliquots at -80°C until analysis. DPP-IV activity was determined using fluorometric assay which measures the release of 4-methoxy-2naphthylamine (Scharpe et al. 1988). All solutions and buffers were prepared the same day. One unit (U) of enzymatic activity is defined as the amount of DPP-IV that cleaves 1µmol of glycyl-L-proline-4-methoxy-2nephthylamine per minute. The assay was repeated again one and two weeks after the first trial. A one-way repeated measure ANOVA was used to test for differences among time points (α=0.05). A coefficient of variation was calculated for each participant and averaged for a total coefficient of variation. RESULTS: Mean DPP-IV activity for Day 1 was 34.57 ± 6.80U/L. One week later, DPP-IV activity had an average of 34.88 ±6.66U/L. The final day mean DPP-IV activity was 32.72 ±7.94U/L two weeks after the initial trial. The ANOVA reported no significant difference (P= 0.85). The total coefficient of variation was 5%. CONCLUSION: Storage up to three weeks results in a stable and reliable DPP-IV activity value when measured using this fluorometric assay. These values fall within normal reference values presented by Durnix et al. (2001). Further studies should investigate if there is a time point past three weeks that yields the same reliability.Concurrent exercise, a combination of endurance and resistance exercise performed in succession, may compromise muscle hypertrophic adaptation induced by resistance training alone. However, little is known about the molecular signaling interactions underlying the change in skeletal muscle adaptation. PURPOSE: To investigate whether endurance exercise (EE) before or after resistance exercise (RE) affects molecular signaling associated with muscle protein synthesis, specifically the interaction between RE-induced mammalian target of rapamycin complex 1 (mTORC1) signaling and EE-induced AMP-activated protein kinase (AMPK) signaling, in an animal model. METHODS: Male Sprague-Dawley rats were divided into 3 groups: EE (treadmill, 25m/min, 60min) before RE (maximum isometric contraction via percutaneous electrical stimulation for 3 sec × 10, 5 sets) group, EE after RE group, or non-e...