Performance verification of a new domestic chemiluminescence detection system

Yang, Lihua; Wang, Lin; Gong, Yuji; Shi, Wen-Pu; An, Xiaohu; Zheng, Huifei

doi:10.21037/atm-20-7047

Cited by 3 publications

(3 citation statements)

References 6 publications

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“…IL-6 has been determined by different approaches, such as chemiluminescent immunoassay, , colorimetric immunoassay, electrochemical immunosensor, − liquid-gated field-effect transistor, fluorescence immunoassay, − and surface plasmon resonance biosensor, − and with the use of aptamers (micro-array, impedance, and UV–vis spectrophotometry) ,,− among others.…”

mentioning

confidence: 99%

On the Move-Sensitive Fluorescent Aptassay on Board Catalytic Micromotors for the Determination of Interleukin-6 in Ultra-Low Serum Volumes for Neonatal Sepsis Diagnostics

et al. 2022

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A graphene oxide/nickel/platinum nanoparticle micromotor (MM)-based fluorescent aptassay is proposed to determine interleukin-6 (IL-6) in serum samples from low-birth-weight infants (gestational age of less than 32 weeks and birthweight below 1000 g) with sepsis suspicion. In this kind of patients, IL-6 has demonstrated good sensitivity and specificity for the diagnosis of sepsis, both for early and late onset sepsis. The approach was based on the adsorption of the aptamer for IL-6 tagged with 6-FAM as a fluorescent label (AptIL‑6, λem = 520 nm) on the graphene oxide external layer (MMGO–AptIL‑6) inducing fluorescence quenching (OFF state) and a subsequent on-the-move affinity recognition of IL-6 from AptIL‑6 (IL-6–AptIL‑6 complex) recovering the fluorescence (ON state). An aptamer against IL-6 was selected and developed by the systematic evolution of ligands by exponential enrichment technology. This approach displayed a suitable linear range of 0.07–1000 pg mL–1 (r = 0.995) covering the cut-off and clinical practice levels, allowing direct determination without any dilution and simplifying the analysis as well as exhibiting an excellent sensitivity (LOD = 0.02 pg mL–1) in ultralow volumes of diagnostic clinical samples (2 μL). A high agreement between IL-6 levels obtained from our MM-based approach and the method used by the Hospital was obtained (relative error < 3%). The MM-based aptassay is competitive in comparison with that of the Hospital, in terms of a significant reduction of the sample volume (15 times less) and enhanced sensitivity, employing similar analysis times. These results position MM technology with enough potential to achieve high sensitivities in low sample volumes, opening new avenues in diagnosis based on low sample volumes.

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confidence: 99%

On the Move-Sensitive Fluorescent Aptassay on Board Catalytic Micromotors for the Determination of Interleukin-6 in Ultra-Low Serum Volumes for Neonatal Sepsis Diagnostics

et al. 2022

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“…These two pyrrolidin-2-ols could be synthesized in very good yields. The reactions of 11 and 14 with elemental sulfur, without any solvent at 200 °C lead to thiones 13 [38] and 16, [39] respectively, in very good yields (Scheme 7). These two reactions validate pyrrolidin-2-ols 11 and 14 as sources of benz-annulated cyclic (alkyl)(amino)carbene (indol-2-ylidene) 12 [40] and cyclic (amino)-(aryl)carbene 15.…”

Section: Angewandte Chemiementioning

confidence: 99%

Realizing 1,1‐Dehydration of Secondary Alcohols to Carbenes: Pyrrolidin‐2‐ols as a Source of Cyclic (Alkyl)(Amino)Carbenes

et al. 2022

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Herein we report secondary pyrrolidin-2-ols as a source of cyclic (alkyl)(amino)carbenes (CAAC) for the synthesis of CAAC-Cu I -complexes and cyclic thiones when reacted with Cu I -salts and elemental sulfur, respectively, under reductive elimination of water from the carbon(IV)-center. This result demonstrates a convenient and facile access to CAAC-based Cu I -salts, which are well known catalysts for different organic transformations. It further establishes secondary alcohols to be a viable source of carbenes-realizing after 185 years Dumas' dream who tried to prepare the parent carbene (CH 2 ) by 1,1-dehydration of methanol. Addressed is also the reactivity of water towards CAACs, which proceeds through an oxidative addition of the OÀ H bond to the carbon(II)-center. This emphasizes the ability of carbon-compounds to mimic the reactivity of transition-metal complexes: reversible oxidative addition and reductive elimination of the OÀ H bond to/from the C(II)/C(IV)-centre.

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“…With the rapid development of laboratory medicine in recent years, various test instruments are available for use in laboratory medicine departments of hospitals, and the same department may even use two or more test instruments for the same purpose ( 22 ). In addition, several chemiluminescence detection systems on the market, but the detecting results can vary significantly between different detection systems, which would produce unreliable results.…”

Section: Introductionmentioning

confidence: 99%

Comparison on the consistency of Mindray and Siemens chemiluminescence analyzers for detecting FT3, FT4 and TSH in patients with hyper- and hypothyroidism

Feng¹,

Wang²,

Zhang³

et al. 2022

Ann Transl Med

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Background: To examine the consistency of the Mindray and Siemens full-automatic chemiluminescence analyzers in detecting serum free triiodothyronine (FT3), free thyroxine (FT4) and thyroid stimulating hormone (TSH) in patients with hyper-or hypothyroidism.Methods: Included in this study were 164 new patients with abnormal thyroid function assessed at Nan Fang Hospital of Southern Medical University in 2021: 107 patients with hyperthyroidism and 57 with hypothyroidism. Additional 100 healthy individuals comprised the control group. Consistency of the FT3, FT4 and TSH results from the two systems in the different groups was analyzed by Kappa, linear correlation, regression, Bland-Altman and area under the receiver operating characteristic curve (AUC).Results: Compared with the control group, FT3 and FT4 levels were increased and TSH level was decreased in the hyperthyroid group (both P<0.05), whereas FT3 and FT4 levels were decreased and TSH level was increased in the hypothyroidism group (both P<0.05), suggesting that both test systems could provide references for relevant clinical evaluation. Kappa analysis showed high consistency of the two systems in detecting FT3, FT4 and TSH in both hyper-and hypothyroidism patients (mean Kappa value >0.7). In addition, there was a good linear correlation between the test results of the two systems (R 2 >0.90).Bland-Altman consistency analysis showed that most mean difference values were within the consistency limit (x±1.96s), indicating that the difference between the two systems was acceptable. AUC of FT3, FT4 and TSH detected by the two systems was higher than 0.80 in each group, showing no significant difference between them (both P>0.05), and the difference in sensitivity and specificity was within the acceptable range.Conclusions: Mindray and Siemens chemiluminescence analyzers are highly consistent in detecting FT3, FT4 and TSH in both hyper-and hypothyroidism patients.

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Performance verification of a new domestic chemiluminescence detection system

Cited by 3 publications

References 6 publications

On the Move-Sensitive Fluorescent Aptassay on Board Catalytic Micromotors for the Determination of Interleukin-6 in Ultra-Low Serum Volumes for Neonatal Sepsis Diagnostics

On the Move-Sensitive Fluorescent Aptassay on Board Catalytic Micromotors for the Determination of Interleukin-6 in Ultra-Low Serum Volumes for Neonatal Sepsis Diagnostics

Realizing 1,1‐Dehydration of Secondary Alcohols to Carbenes: Pyrrolidin‐2‐ols as a Source of Cyclic (Alkyl)(Amino)Carbenes

Comparison on the consistency of Mindray and Siemens chemiluminescence analyzers for detecting FT3, FT4 and TSH in patients with hyper- and hypothyroidism

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