Abstract-In order to investigate the effect of PBL(problem-based learning) combined with standardized patient in the teaching of ophthalmology, 64 students were divided randomly into experimental group(n=32) and control group(n=32). Traditional teaching method was implemented in control group while PBL combined with SP was applied in experimental group. At the end of term students were interviewed using self-administered questionnaire to obtain their evaluation for teaching effect. Enumeration data were analyzed by χ2 test. Measurement data were expressed as x± s and analyzed by independent samples t test. P<0.05 signifies statistical differences. The students in experimental group had a significantly better self-assessment than the control group in learning enthusiasm, consciousness of team cooperation, comprehensive analysis ability, self-learning ability, innovation ability and practice ability.