Performance parameters of the Hamburg 4 pi whole body radioactivity detector

Braunsfurth, J.; Gabbe, E. E.; Heinrich, H. C.

doi:10.1088/0031-9155/22/1/002

Cited by 16 publications

(11 citation statements)

References 14 publications

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“…Uptake was calculated as percentage of activity in all measured organs. 59 Fe-activity was measured using the large volume Hamburg whole body radioactivity counter [27]. In vivo imaging studies.…”

Section: Experimental Autoimmune Encephalomyelitis 200 µG Mbp Peptidmentioning

confidence: 99%

Nanoparticle-based autoantigen delivery to Treg-inducing liver sinusoidal endothelial cells enables control of autoimmunity in mice

Carambia

Freund

Schwinge

et al. 2015

Journal of Hepatology

150

143

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Section: Experimental Autoimmune Encephalomyelitis 200 µG Mbp Peptidmentioning

confidence: 99%

Nanoparticle-based autoantigen delivery to Treg-inducing liver sinusoidal endothelial cells enables control of autoimmunity in mice

Carambia

Freund

Schwinge

et al. 2015

Journal of Hepatology

150

143

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“…For the gamma activity measurements performed in Hamburg, a whole-body radioactivity counter (HAMCO) 20 was used. The iron oxide core was prelabeled with 59 Fe (NP-HDL 59Fe-SPIO ).…”

Section: Determination Of Blood Clearance and Biodistribution Of Np-hmentioning

confidence: 99%

“…The total amount of applied radioactivity was measured immediately after injection. Blood samples were taken 1,5,20,40,60,90,120,180, and 220 minutes after injection to determine blood clearance. For the biodistribution of NP-HDL 59FeSPIO , the mice were perfused with 4% paraformaldehyde 220 minutes postinjection, and activity measurements were performed on the aorta, spleen, kidney, lung, muscle, heart, liver, peritoneal macrophages, and femur.…”

Section: Determination Of Blood Clearance and Biodistribution Of Np-hmentioning

confidence: 99%

Intraperitoneal Injection Improves the Uptake of Nanoparticle-Labeled High-Density Lipoprotein to Atherosclerotic Plaques Compared With Intravenous Injection

Jung

Kaul

Bruns

et al. 2014

Circ: Cardiovascular Imaging

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A therosclerosis remains the main cause of morbidity and mortality in industrialized and developing nations. 1 Growing evidence suggests that the decisive factor determining atherosclerotic plaque vulnerability is the plaque configuration rather than the degree of luminal narrowing. 2Molecular imaging may offer a new diagnostic tool to discriminate stable and vulnerable atherosclerotic plaques at an early stage of disease and to improve the detection of morphological and functional abnormalities within the vessel wall. 3,4 The major hallmarks of early atherosclerotic lesion formation are endothelial dysfunction and accumulation of large amounts of lipoprotein-derived cholesterol esters in macrophages within Background-The aim of this study was to assess whether high-density lipoprotein (HDL) labeled with superparamagnetic iron oxide nanoparticles (SPIOs) and quantum dots was able to detect atherosclerotic lesions in mice after intravenous and intraperitoneal injection by multimodal imaging. Methods and Results-Nanoparticle-labeled HDLs (NP-HDLs) were characterized in vitro by dynamic light scattering and size exclusion chromatography with subsequent cholesterol and fluorescence measurements. For biodistribution and blood clearance studies, NP-HDL SPIOs radiolabeled with 59Fe (NP-HDL 59Fe-SPIOs ) were injected intravenously or intraperitoneally into ApoE knockout mice (n=6), and radioactivity was measured using a gamma counter. NP-HDL accumulation within atherosclerotic plaques in vivo and ex vivo was estimated by MRI at 7 Tesla, ex vivo confocal fluorescence microscopy, x-ray fluorescence microscopy, and histological analysis (n=3). Statistical analyses were performed using a 2-tailed Student t-test. In vitro characterization of NP-HDL confirmed properties similar to endogenous HDL. Blood concentration time curves showed a biexponential decrease for the intravenous injection, whereas a slow increase followed by a steady state was noted for intraperitoneal injection. Radioactivity measurements showed predominant accumulation in the liver and spleen after both application approaches. NP-HDL 59Fe-SPIOs uptake into atherosclerotic plaques increased significantly after intraperitoneal compared with intravenous injection (P<0.01). In vivo MRI showed an increased uptake of NP-HDL into atherosclerotic lesions after intraperitoneal injection, which was confirmed by ex vivo MRI, x-ray fluorescence microscopy, confocal fluorescence microscopy, and histological analysis. Conclusions-In vivo MRI and ex vivo multimodal imaging of atherosclerotic plaque using NP-HDL is feasible, and intraperitoneal application improves the uptake within vessel wall lesions compared with intravenous injection. (Circ Cardiovasc Imaging. 2014;7:303-311.)

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“…The activity, measured immediately after administration of 51 Cr, was arbitrarily set as the 100% reference value. The 51 Cr whole-body retention was measured at given timepoints in the centre of a 200 cm long 4p-geometry whole-body radioactivity detector with liquid organic scintillator in the energy range from 980-3,000 keV (Braunsfurth et al 1977). The biological half-life of 51 Cr was calculated from a triple term exponential fit algorithm to the measured wholebody retention of 51 Cr values in a period of 1-100 days after the administration of 51 Cr.…”

Section: Animal Experimentsmentioning

confidence: 99%

Absorption, excretion and retention of 51Cr from labelled Cr-(III)-picolinate in rats

et al. 2008

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The bioavailability of chromium from Cr-picolinate (CrPic(3)) and Cr-chloride (CrCl(3)) was studied in rats using (51)Cr-labelled compounds and whole-body-counting. The intestinal absorption of Cr was twice as high from CrPic(3) (1.16% vs 0.55%) than from CrCl(3), however most of the absorbed (51)Cr from CrPic(3) was excreted into the urine within 24 h. After i.v. or i.p. injection, the whole-body retention curves fitted well to a multiexponential function, demonstrating that plasma chromium is in equilibrium with three pools. For CrPic(3), a large pool exists with a very rapid exchange (T (1/2) = <0.5 days), suggesting that CrPic(3) is absorbed as intact molecule, from which the main part is directly excreted by the kidney before degradation of the chromium complex in the liver can occur. CrCl(3) is less well absorbed but the rapid exchange pool is much smaller, resulting in even higher Cr concentrations in tissue such as muscle and fat. However, 1-3 days after application, the relative distribution of (51)Cr from both compounds was similar in all tissues studied, indicating that both compounds contribute to the same storage pool. In summary, the bioavailability of CrPic(3) in rats is not superior compared to CrCl(3).

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Performance parameters of the Hamburg 4 pi whole body radioactivity detector

Cited by 16 publications

References 14 publications

Nanoparticle-based autoantigen delivery to Treg-inducing liver sinusoidal endothelial cells enables control of autoimmunity in mice

Nanoparticle-based autoantigen delivery to Treg-inducing liver sinusoidal endothelial cells enables control of autoimmunity in mice

Intraperitoneal Injection Improves the Uptake of Nanoparticle-Labeled High-Density Lipoprotein to Atherosclerotic Plaques Compared With Intravenous Injection

Absorption, excretion and retention of 51Cr from labelled Cr-(III)-picolinate in rats

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