Performance of UVAPS with respect to detection of airborne fungi

Kanaani, Hussein; Hargreaves, Megan; Smith, James J.; Ristovski, Zoran; Agranovski, Victoria; Morawska, Lidia

doi:10.1016/j.jaerosci.2007.10.007

Cited by 61 publications

(47 citation statements)

References 27 publications

(39 reference statements)

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“…It was noted that the higher the inlet velocity (or the ventilation rate), the higher was the air jet momentum (Chow and Wong 1998c). Increased deposition was due to increased particle collisions with the chamber walls (Kanaani et al 2008b).…”

Section: Resultsmentioning

confidence: 99%

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An Experimental and Numerical Study on Deposition of Bioaerosols in a Scaled Chamber

Wong

Chan

Mui

et al. 2010

Aerosol Science and Technology

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This work presents an experimental facility designed and built with the objective of understanding the deposition of bioaerosols in indoor environments. Multiple depositions of two microorganisms Staphylococcus and Micrococcus inside a test chamber were investigated under two air mixing conditions. Airflow rate was demonstrated to have an influence on the concentration homogeneity. An increased proportion of particle deposition was found in the floor section near the chamber wall opposite to the air inlet when air mixing was not enhanced by the mixing fans. Both the experimental results and Eulerian-Lagrangian computations revealed that a small mixing fan inside the chamber prompted very effective mixing while non-homogeneity was observed even at a very high ventilation rate. The results showed that both ventilation rate and mixing conditions in the ventilated chamber have influence on the bioaerosol dispersion and deposition.

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Section: Resultsmentioning

confidence: 99%

“…• C under aerobic conditions for another 24 h. A method of harvesting aerosolized bacteria followed (Kanaani et al 2008b). For aerosolization, a 50 ml bacterial suspension was stored in the collision nebulizer "c" (Figure 1).…”

Section: Methodsmentioning

confidence: 99%

An Experimental and Numerical Study on Deposition of Bioaerosols in a Scaled Chamber

Wong

Chan

Mui

et al. 2010

Aerosol Science and Technology

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“…The dry generation method, which was described in detail by Kanaani et al (2006), was used for fungal spore release. In this method, the compressed air was filtered by a HEPA filter and introduced into the mixing chamber at a flow rate of 10 L/min in all the cases, except for the two-day-old cultures for which a flow rate of 20 L/min was used.…”

Section: The Fungal Spore Size and Fluorescent Percentage As A Functimentioning

confidence: 99%

“…Work by Kanaani et al(2006), addressed the efficiency and the limits of the UVAPS in detecting fungal spores, showing the ability of the instrument in detecting and measuring fungal spores and that its upper detection-limit (the point beyond which the relationship between total and fluorescent particle concentration cease to be linear) of fluorescent particles was around 7 × 10 7 particles/m 3 . However, there is still a need for further characterisation and validation of the instrument, and more research is necessary to develop a full understanding of the capabilities and limitations its application, for studies of airborne fungal spores.…”

Section: Introductionmentioning

confidence: 99%

Performance assessment of UVAPS: Influence of fungal spore age and air exposure

Kanaani

Hargreaves

Ristovski

et al. 2007

Journal of Aerosol Science

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This work focused on two main outcomes. The first was the assessment of the response of the Ultraviolet Aerodynamic Particle Sizer Spectrometer (UVAPS) for two different fungal spore species.The UVAPS response was investigated as a function of fungal age and the frequency of air current that their colonies exposure to. This outcome was achieved through the measurement of fungal spore fluorescent percentage and fluorescent intensity throughout a period of culturing time (three weeks), and the study of their fluorescent percentage as a function of exposure to air currents. The second objective was to investigate the change of fungal spore size during this period, which may be of use as a co-factor in this differentiation. Fungal spores were released by blowing the surface of the culture colonies with continuous filtered flow air. The UVAPS was used to detect and measure autofluorescing biomolecules such as riboflavin and nicotinamide adenine dinucleotide phosphate (NAD(P)H) present in the released fungal spores.The study demonstrated an increase in aerodynamic diameter for fungal spores under investigation (Aspergillus niger and Penicillium species) over a period of time. The fluorescent percentage of spores was found to decrease for both fungal genera as they aged. It was also found that the fluorescent percentage for tested fungi decreased with frequency of air exposure. The results showed that, while the UVAPS could discriminate between Aspergillus and Penicillium species under well-controlled laboratory conditions, it is unlikely to be able to do so in the field.

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“…Fluorescent polystyrene latex spheres (PSLs) play a particularly important role in the characterization and calibration of instruments that rely on particle autofluorescence (also called intrinsic fluorescence) detection. For example, PSLs are commonly used for testing and calibration of instruments such as flow cytometers and lightinduced fluorescent (LIF) instruments (e.g., Hasegawa, 2013;Healy et al, 2012;Jung et al, 2012;Kanaani et al, 2008;Robinson et al, 2017;Toprak and Schnaiter, 2013).…”

Section: Introductionmentioning

confidence: 99%

Characterization of steady-state fluorescence properties of polystyrene latex spheres using off- and online spectroscopic methods

et al. 2018

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Abstract. Fluorescent dyed polystyrene latex spheres (PSLs) are commonly used for characterization and calibration of instruments detecting fluorescence signals from particles suspended in the air and other fluids. Instruments like the Ultraviolet Aerodynamic Particle Sizer (UV-APS) and the Waveband Integrated Bioaerosol Sensor (WIBS) are widely used for bioaerosol research, but these instruments present significant technical and physical challenges requiring careful characterization with standard particles. Many other research communities use flow cytometry and other instruments that interrogate fluorescence from individual particles, and these also frequently rely on fluorescent PSLs as standards. Nevertheless, information about physical properties of commercially available PSLs provided by each manufacturer is generally proprietary and rarely available, making their use in fluorescence validation and calibration very difficult.This technical note presents an overview of steady-state fluorescence properties of fluorescent and non-fluorescent PSLs, as well as of polystyrene-divinylbenzene (PS-DVB) particles, by using on-and offline spectroscopic techniques. We show that the "fluorescence landscape" of PSLs is more complex than the information typically provided by manufacturers may imply, especially revealing multimodal emission patterns. Furthermore, non-fluorescent PSLs also exhibit defined patterns of fluorescent emission originating from a mixture of polystyrene and detergents, which becomes a crucial point for fluorescence threshold calibrations and qualitative comparison between instruments. By comparing PSLs of different sizes, but doped with the same dye, changes in emission spectra from bulk solutions are not immediately obvious. On a single-particle scale, however, fluorescence intensity values increase with increasing particle size. No significant effect in the fluorescence signatures was detectable by comparing PSLs in dry vs. wet states, indicating that solvent water may only play a minor role as a fluorescence quencher.Because information provided by manufacturers of commercially available PSLs is generally very limited, we provide the steady-state excitation-emission matrices (EEMs) of PSLs as open-access data within the Supplement. Detergent and solvent effects are also discussed in order to provide information not available elsewhere to researchers in the bioaerosol and other research communities. These data are not meant to serve as a fundamental library of PSL properties because of the variability of fluorescent properties between batches and as a function of particle aging and agglomeration. The data presented, however, provide a summary of spectral features which are consistent across these widely used fluorescent standards. Using these concepts, further checks will likely be required by individual researchers using specific lots of standards.

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Performance of UVAPS with respect to detection of airborne fungi

Cited by 61 publications

References 27 publications

An Experimental and Numerical Study on Deposition of Bioaerosols in a Scaled Chamber

An Experimental and Numerical Study on Deposition of Bioaerosols in a Scaled Chamber

Performance assessment of UVAPS: Influence of fungal spore age and air exposure

Characterization of steady-state fluorescence properties of polystyrene latex spheres using off- and online spectroscopic methods

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