Performance of trichlorfon degradation by a novel Bacillus tequilensis strain PA F-3 and its proposed biodegradation pathway

Abstract

The novel trichlorfon (TCF)-degrading bacterium PA F-3, identified as Bacillus tequilensis, was isolated from pesticide-polluted soils by using an effective screening and domesticating procedure. The TCF biodegradation pathways of PA F-3 were also systematically elucidated. As revealed by high-performance liquid chromatography, the TCF residues in the mineral salt medium demonstrated that PA F-3 can utilize TCF as its sole carbon source and reach the highest degradation of 71.1 % at an initial TCF concentration of 200 mg/L within 5 days. The TCF degradation conditions were optimized using response surface methodology as follows: temperature, 28 °C; inoculum amount, 4 %; and initial TCF concentration, 125 mg/L. Biodegradation treatments supplemented with exogenous carbon sources and yeast extract markedly increased the microbial dry weights and TCF-degrading performance of PA F-3, respectively. Meanwhile, five metabolic products of TCF were identified through gas chromatography/mass spectrometry, and a biodegradation pathway was proposed. Results indicated that deoxidation and dehydration (including the cleavage of the P-C phosphonate bond and the C-O bond) were the preferred metabolic reactions of TCF in this TCF-degrading bacterium.

Toxicity, Biodegradation, and Metabolic Fate of Organophosphorus Pesticide Trichlorfon on the Freshwater Algae Chlamydomonas reinhardtii

“…The retention time, m / z , and chemical structure of each metabolite are listed in Table . Among these metabolites, chloral, dimethyl phosphite, dimethyl hydrogen phosphite, methyl phosphate, and chloral hydrate have also been reported during the biodegradation of TCF by fungi and bacteria …”

“…16 Previous studies have reported the microbial remediation of TCF by bacteria and fungi. 15,17 However, to the best of our knowledge, the biodegradation of TCF by algae and the biodegradation pathways of TCF by algae have not been reported. In addition, the mechanism of action of TCF on algae is still not fully understood.…”

“…Microbial remediation is a relatively effective and safe process because it can decompose harmful organic pollutants without harming the environment . Previous studies have reported the microbial remediation of TCF by bacteria and fungi. , However, to the best of our knowledge, the biodegradation of TCF by algae and the biodegradation pathways of TCF by algae have not been reported. In addition, the mechanism of action of TCF on algae is still not fully understood.…”

Metabolic Profiling and Comparative Proteomic Insight in Respect of Amidases during Iprodione Biodegradation

“…However, biomass growth is usually inhibited by the presence of pesticides, as reported for high concentrations (>400 mg L −1 ) [44,45]. This response can be countered by the addition of other carbon sources into the liquid culture medium rather than decreasing the concentration of the contaminant [46]. According to our observation, at the same IPR concentration, the presence of a double amount of nutrients can accelerate IPR removal, as we observed by comparing our results with those obtained by Briceño et al [15], where a diluted LB medium was used.…”

Microbe mediated remediation of dyes, explosive waste and polyaromatic hydrocarbons, pesticides and pharmaceuticals

“… CLSM GC-ECD ( Feng et al., 2017 ) Chryseobacteriumindologenes Strain SSJ1 flubendiamide samples were collected from groundnut cultivating soil of Dharwad district, Karnataka, India, isolate utilized the flubendiamide as a sole carbon and nitrogen source – 89.06% initial pesticide was removed by the isolate with 5 days incubation period UV–vis Spectroscopy, HPLC ( Jadhav and David, 2016 ) Bacillus sp. Strain SG2 cypermethrin Pesticide-contaminated soils were collected from a rice field of Udham Singh Nagar, Uttarakhand, India ester hydrolysis of pyrethroid takes place by carboxylesterases, results in acid and alcohol production 4-propylbenzoate, 4-propylbenzaldehyde, phenol M-tertbutyl and 1-dodecanol bacteria degraded the compound up to 81.6% within 15 days GC–MS FTIR ( Sharma et al., 2016 ) Bacillus tequilensis trichlorfon (TCF) Soil samples were collected from the surface layer of a pesticide-polluted field in Hubei Province, China deoxidation and dehydration (including the cleavage of the P–C phosphonate bond and the C–O bond) DDCV, dimethyl phosphite, Trichloroethanal, dimethyl hydrogen phosphite and chloral hydrate degradation of 71.1% at an initial TCF concentration of 200 mg/L within 5 days HPLC GC–MS ( Tian et al., 2016 ) Bacillus subtilis and Fomitopsispinicola DDT Culture collection (1) dechlorination to DDD, (2) dehydrochlorination to DDE, and (3) formation of DDMU DDD (1,1-dichloro- 2,2-bis(4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4- chlorophenyl) ethylene), and DDMU (1‑chloro-2,2-bis(4-chlorophenyl) ethylene) addition of 10 mL of B. subtilis into F. pinicola culture showed the highest DDT degradation of 86% during the 7days incubation period HPLC GC–MS ( Sariwati et al., 2017 ) Citricoccus sp. TT3 Atrazine Soil samples were collected from the wastewater outfall of the Tianjin Huayu Pesticide Facto...…”