Performance of the trial-unique, delayed non-matching-to-location (TUNL) task depends on AMPA/Kainate, but not NMDA, ionotropic glutamate receptors in the rat posterior parietal cortex

Scott, Gavin A.; Roebuck, Andrew J.; Greba, Quentin; Howland, John G.

doi:10.1016/j.nlm.2019.02.001

Cited by 17 publications

(26 citation statements)

References 58 publications

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“…All habituation, pretraining and training stages were conducted according to protocols developed by Lafayette, and previous experiments conducted in our lab 4,26‐28 . All stages of TUNL occurred once daily, 5‐7 days per week.…”

Section: Methodsmentioning

confidence: 99%

“…Importantly, we omitted correction trials from ICES testing. We have previously used correction trials as a measure of behavioral flexibility 4 . However, we sought to maximize the number of selection trials rats performed in a session in order to obtain sufficient numbers of each stim trial type.…”

Section: Methodsmentioning

confidence: 99%

“…The posterior parietal cortex (PPC) is an area of association cortex in the posterior cerebrum that is critical for a range of short‐term memory‐guided cognitive processes in primates and rodents including working memory (WM), 1‐12 perceptually guided decision making, 13 and sensory evidence accumulation 14,15 . Despite this evidence, however, the precise role of the rodent PPC in these cognitive processes is unclear.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we showed that the rat PPC is critical for performance of the Trial‐Unique, Nonmatching‐to‐Location (TUNL) task 4 (Figure 1), a touchscreen‐based visuospatial WM task for rodents 23‐25 . Although our results clearly show that the PPC is critical for TUNL performance, the TUNL task involves several phases including the presentation of the initial sample stimulus, the delay period in which the location of the sample stimulus must be remembered, and the test phase in which rats choose between the sample and test stimuli.…”

Section: Introductionmentioning

confidence: 99%

“…Importantly, the configuration of the TUNL task also allows the separate manipulation of neural activity during sample stimulus encoding, delay maintenance, and comparing and choosing between test stimuli. Previous research has either used tasks configured so that these processes overlap in time 21 or have performed manipulations affecting all task phases 4,14,22 …”

Section: Introductionmentioning

confidence: 99%

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Task phase‐specific involvement of the rat posterior parietal cortex in performance of the TUNL task

Scott

Cai

Song

et al. 2020

Genes Brain and Behavior

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The posterior parietal cortex (PPC) participates in cognitive processes including working memory (WM), sensory evidence accumulation, and perceptually guided decision making. However, surprisingly little work has used temporally precise manipulations to dissect its role in different epochs of behavior taking place over short timespans, such as WM tasks. As a result, a consistent view of the temporally precise role of the PPC in these processes has not been described. In the present study, we investigated the temporally specific role of the PPC in the Trial‐Unique, Nonmatching‐to‐Location (TUNL) task, a touchscreen‐based, visuospatial WM task that relies on the PPC. To disrupt PPC activity in a temporally precise manner, we applied mild intracranial electrical stimulation (ICES). We found that intra‐PPC ICES (100 μA) significantly impaired accuracy in TUNL without significantly altering response latency. Moreover, we found that the impairment was specific to ICES applied during the delay and test phases of TUNL. Consistent with previous reports showing delay‐ and choice‐specific neuronal activity in the PPC, the results provide evidence that the rat PPC is required for maintaining memory representations of stimuli over a delay period as well as for making successful comparisons and choices between test stimuli. In contrast, the PPC appears not to be critical for initial encoding of sample stimuli. This pattern of results may indicate that early encoding of visual stimuli is independent of the PPC or that the PPC becomes engaged only when visual stimuli are spatially complex or involve memory or decision making.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Task phase‐specific involvement of the rat posterior parietal cortex in performance of the TUNL task

Scott

Cai

Song

et al. 2020

Genes Brain and Behavior

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The Touchscreen‐Based Trial‐Unique, Nonmatching‐To‐Location (TUNL) Task as a Measure of Working Memory and Pattern Separation in Rats and Mice

et al. 2021

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The TUNL task is an automated touchscreen task used to evaluate the cognitive processes involved in working memory (WM) and spatial pattern separation in rodents. Both rats and mice can be used. To elicit working memory processes, the rodent must distinguish between a sample (familiar) light stimulus and a novel light stimulus after a delay. With a correct selection, the rodent will receive a food reward. A major benefit of TUNL compared to other similar tasks is the circumvention of spatial "mediating strategies" that the rodent may use to supplement or replace working memory processes to complete the task successfully. Each trial is 'unique', as the stimuli are pseudo-randomized between trials in an array of spatial locations. The TUNL task uses a progression of six training steps to teach the rodent the associated rules necessary to complete the full task. Task performance is typically measured by trials completed and by accuracy. Task accuracy can be evaluated across various spatial separations to engage hippocampal-dependent processes involved in spatial pattern separation. The latency between trial responses can also be evaluated, with food reward collection latency as a measure of motivation. The TUNL task can be used to assess working memory and cognitive deficits in rodent models with neurodegenerative and neurological disorders, providing a valuable tool to screen for new treatment options, in addition to assessing basic neurobiology.

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Dopaminergic psychostimulants cause arousal from isoflurane-induced sedation without reversing memory impairment in rats

Fettiplace,

Vincent,

Cho

et al. 2024

British Journal of Anaesthesia

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Performance of the trial-unique, delayed non-matching-to-location (TUNL) task depends on AMPA/Kainate, but not NMDA, ionotropic glutamate receptors in the rat posterior parietal cortex

Cited by 17 publications

References 58 publications

Task phase‐specific involvement of the rat posterior parietal cortex in performance of the TUNL task

Task phase‐specific involvement of the rat posterior parietal cortex in performance of the TUNL task

The Touchscreen‐Based Trial‐Unique, Nonmatching‐To‐Location (TUNL) Task as a Measure of Working Memory and Pattern Separation in Rats and Mice

Dopaminergic psychostimulants cause arousal from isoflurane-induced sedation without reversing memory impairment in rats

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