Performance of the Roche/Snibe electrochemiluminescent anti-SARS-COV-2 spike assays compared to the Roche/Abbott IgG nucleocapsid and Abbott IgM spike assays

Lau, Chin Shern; Wong, Melissa S.; Hoo, S.P.; Heng, P.Y.; Phua, Soon Kieng; Aw, Tar Choon

doi:10.1016/j.plabm.2021.e00257

Cited by 1 publication

(1 citation statement)

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“…Thawed samples were vortexed before analysis. The Roche Elecsys Anti-SARS-CoV-2 S quantitative double-antigen sandwich electro-chemiluminescent immunoassay (run on the Roche Elecsys e801 auto-analyzer) and the Snibe competitive quantitative N-Ab assay (run on the Snibe Maglumi) have been previously evaluated and described by our laboratory [11,12]. The Snibe N-Ab assay reports a clinical sensitivity of 100% (95% CI 93.69-100) in 57 samples with confirmed SARS-CoV-2 VNT 50 ≥ 20 in their manufacturer's insert.…”

Section: Methodsmentioning

confidence: 99%

SARS-CoV-2 Spike and Neutralizing Antibody Kinetics 90 Days after Three Doses of BNT162b2 mRNA COVID-19 Vaccine in Singapore

et al. 2022

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Background: We evaluated the post-booster (BNT162b2) antibody responses in Singapore. Methods: Participants (n = 43) were tested pre-booster and 20/30/60/90 days post-booster. Participants were boosted 120–240 days (mean 214 days) after their second dose and had no history or serologic evidence of prior COVID-19 infection; all participants had undetectable SARS-CoV-2 nucleocapsid antibodies throughout the study. Total nucleocapsid and spike antibodies (S-Ab) were assessed on the Roche Elecsys e802 and neutralizing antibody (N-Ab) on the Snibe quantitative N-Ab assay. Results: Pre-booster median S-Ab/N-Ab titers were 829 BAU/mL/0.83 µg/mL; 2 participants were below manufacturer’s N-Ab cut-offs of 0.3 µg/mL (0.192 and 0.229). Both S-Ab and N-Ab titers peaked at 30 days post-booster (median S-Ab 25,220 BAU/mL and N-Ab 30.3 µg/mL) at 30–37× pre-booster median levels. These peak post-booster S-Ab/N-Ab titers were 11× (25,220 vs. 2235 BAU/mL) and 9× (30.3 vs. 3.52 µg/mL) higher than the previously reported peak post-second dose levels. Antibody titers declined to 12,315 BAU/mL (51% decrease) and 14.3 µg/mL (53% decrease) 90 days post-booster. Non-linear regression estimates for S-Ab/N-Ab half-lives were 44/58 days. At 180 days post-booster, S-Ab/N-Ab are estimated to be 2671 BAU/mL/4.83 µg/mL. Conclusions: Both S-Ab and N-Ab show a good response following post-booster vaccination, with half-lives that may provide a prolonged antibody response.

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Section: Methodsmentioning

confidence: 99%