Performance of the Focus and Kalon Enzyme-Linked Immunosorbent Assays for Antibodies to Herpes Simplex Virus Type 2 Glycoprotein G in Culture-Documented Cases of Genital Herpes

Morrow, Rhoda Ashley; Friedrich, David; Krantz, Elizabeth M

doi:10.1128/jcm.41.11.5212-5214.2003

Cited by 48 publications

(41 citation statements)

References 7 publications

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“…Thirty-seven of the donors were HSV-2 seropositive. Infection with HSV-1 and HSV-2 was diagnosed by type-specific immunoblot (1) or glycoprotein G-based (15) immunoassays. HLA typing was performed by lowresolution PCR-based methods at Puget Sound Blood Center, Seattle, WA.…”

Section: Methodsmentioning

confidence: 99%

Diversity of the CD8⁺T-Cell Response to Herpes Simplex Virus Type 2 Proteins among Persons with Genital Herpes

Hosken

McGowan

Meier

et al. 2006

J Virol

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Cytolytic T cells play a major role in controlling herpes simplex virus type 2 (HSV-2) infections in humans. In an effort to more thoroughly evaluate the response to HSV-2 directly, ex vivo, we developed an enzyme-linked immunospot (ELISPOT) assay that utilized pools of overlapping synthetic peptides presented by autologous dendritic cells to purified CD8 ؉ T cells. Donor response rates to individual open reading frames (ORFs) ranged from fewer than 5% responding to as many as 70% responding, with the greatest frequency of responses (by ORF) being directed against UL39, UL25, UL27, ICP0, UL46, and UL47 in descending order of frequency. HSV-2-seropositive subjects responded to as few as 3 or as many as 46 of the 48 ORFs tested, with a median of 11 ORFs recognized. HLA-B*07 expression correlated with stronger responses overall that were directed primarily against UL49 and UL46. Cumulative precursor frequencies in the blood ranged from 500 to almost 6,000 HSV-2 spot-forming units/10 6 CD8 ؉ T cells. The magnitude and breadth of the response in the infected population were greater than previously appreciated. Whether this variability in the CD8 ؉ T-cell response within individuals is associated with the frequency of viral reactivation warrants further study. Several lines of evidence support the importance of T-cell immunity in the resolution of herpes simplex virus (HSV) infections in humans.Patients with T-cell immune deficiencies, whether genetic or acquired, suffer more frequent reactivation and greater disease severity than immunocompetent persons (8). In animal models, deficiencies in CD8 ϩ T cells reduce viral clearance in neural tissue (16,18). In previous studies, we have shown that the reduction of viral shedding in lesional biopsies coincides with the appearance of HSV-2-specific cytolytic activity in lesions (13). These observations have largely been based upon qualitative assays of T-cell immunity. The last 5 years has seen the development of standardized assays to quantify T-cell responses to viral proteins. While a multitude of these studies have used whole viral antigens to evaluate responses to HSV-1 and HSV-2, little is known about the breadth and magnitude of the response to specific HSV proteins or about how these responses differ between individuals. To explore these issues, we initiated a study to define the predominant responses to specific HSV proteins among HSV-2-seropositive persons.(This work was presented in part at the 28th International Herpesvirus Workshop, Madison, Wis., 2003 and the 27th International Herpesvirus Workshop, Cairns, Australia, 2002.) MATERIALS AND METHODSHuman subjects. Forty donors were recruited for this trial: 33 were attendees of the Virology Research Clinic at the University of Washington, Seattle, and 7 were recruited at Corixa Corp. Subjects electing to participate in the study gave informed consent. Thirty-seven of the donors were HSV-2 seropositive. Infection with HSV-1 and HSV-2 was diagnosed by type-specific immunoblot (1) or glycoprotein G-based (15) immuno...

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Section: Methodsmentioning

confidence: 99%

Diversity of the CD8⁺T-Cell Response to Herpes Simplex Virus Type 2 Proteins among Persons with Genital Herpes

Hosken

McGowan

Meier

et al. 2006

J Virol

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show abstract

“…Western blot testing is not commercially available, but several commercial assays are available which show high sensitivity and specificity in sexually active adults (154), but positive predictive value can still be diminished when used as a screening tool (155). Pending further studies and the availability of future prevention strategies such as vaccines, the utility, cost effectiveness and acceptability of routine screening of asymptomatic HSV infection in MSM attending GUM clinics remains to be determined.…”

Section: Herpes Simplex Virusmentioning

confidence: 99%

2016 United Kingdom national guideline on the sexual health care of men who have sex with men

et al. 2018

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“…One study reported that the sensitivity of HerpeSelect increased for patients suffering from genital ulcer disease (GUD) compared to that for blood donors (6), perhaps because HerpeSelect may detect primary genital herpes earlier than the Kalon assay or even WB does (1,12).…”

mentioning

confidence: 99%

Performance of HerpeSelect and Kalon Assays in Detection of Antibodies to Herpes Simplex Virus Type 2

et al. 2008

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Performance of the Focus and Kalon Enzyme-Linked Immunosorbent Assays for Antibodies to Herpes Simplex Virus Type 2 Glycoprotein G in Culture-Documented Cases of Genital Herpes

Cited by 48 publications

References 7 publications

Diversity of the CD8⁺T-Cell Response to Herpes Simplex Virus Type 2 Proteins among Persons with Genital Herpes

Diversity of the CD8⁺T-Cell Response to Herpes Simplex Virus Type 2 Proteins among Persons with Genital Herpes

2016 United Kingdom national guideline on the sexual health care of men who have sex with men

Performance of HerpeSelect and Kalon Assays in Detection of Antibodies to Herpes Simplex Virus Type 2

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Performance of the Focus and Kalon Enzyme-Linked Immunosorbent Assays for Antibodies to Herpes Simplex Virus Type 2 Glycoprotein G in Culture-Documented Cases of Genital Herpes

Cited by 48 publications

References 7 publications

Diversity of the CD8+T-Cell Response to Herpes Simplex Virus Type 2 Proteins among Persons with Genital Herpes

Diversity of the CD8+T-Cell Response to Herpes Simplex Virus Type 2 Proteins among Persons with Genital Herpes

2016 United Kingdom national guideline on the sexual health care of men who have sex with men

Performance of HerpeSelect and Kalon Assays in Detection of Antibodies to Herpes Simplex Virus Type 2

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Diversity of the CD8⁺T-Cell Response to Herpes Simplex Virus Type 2 Proteins among Persons with Genital Herpes

Diversity of the CD8⁺T-Cell Response to Herpes Simplex Virus Type 2 Proteins among Persons with Genital Herpes