Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures

Bach, Katharina; Edel, Birgit; Höring, Steffen; Bartonickova, Lucie; Glöckner, Stefan; Löffler, Bettina; Bahrs, Christina; Rödel, Jürgen

doi:10.1007/s10096-021-04383-3

Cited by 3 publications

(4 citation statements)

References 27 publications

(33 reference statements)

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“…In turn, Bach et al [ 12 ] assessed the usefulness of the LAMP method-based assay in the identification of selected bacterial species and genes encoding ESBL enzymes from the CTX-M1 and CTX-M9 groups from positive blood cultures. The study included 449 positive blood cultures.…”

Section: Discussionmentioning

confidence: 99%

The Evaluation of Eazyplex® SuperBug CRE Assay Usefulness for the Detection of ESBLs and Carbapenemases Genes Directly from Urine Samples and Positive Blood Cultures

Sękowska

Bogiel

2022

Antibiotics

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Increasing antimicrobial resistance of Gram-negative rods is an important diagnostic, clinical and epidemiological problem of modern medicine. Therefore, it is important to detect multi-drug resistant strains as early on as possible. This study aimed to evaluate Eazyplex® SuperBug CRE assay usefulness for beta-lactamase gene detection among Gram-negative rods, directly from urine samples and positive blood cultures. The Eazyplex® SuperBug CRE assay is based on a loop-mediated isothermal amplification of genetic material and allows for the detection of a selection of genes encoding carbapenemases, KPC, NDM, VIM, OXA-48, OXA-181 and extended-spectrum beta-lactamases from the CTX-M-1 and CTX-M-9 groups. A total of 120 clinical specimens were included in the study. The test gave valid results for 58 (96.7%) urine samples and 57 (95.0%) positive blood cultures. ESBL and/or carbapenemase enzymes genes were detected in 56 (93.3%) urine and 55 (91.7%) blood samples, respectively. The Eazyplex® SuperBug CRE assay can be used for a rapid detection of the genes encoding the most important resistance mechanisms to beta-lactams in Gram-negative rods also without the necessity of bacterial culture.

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Section: Discussionmentioning

confidence: 99%

The Evaluation of Eazyplex® SuperBug CRE Assay Usefulness for the Detection of ESBLs and Carbapenemases Genes Directly from Urine Samples and Positive Blood Cultures

Sękowska

Bogiel

2022

Antibiotics

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“…There have been many promising attempts to increase the speed of pathogen detection in positive BCs using culture-independent methods. Several PCR-based tests (e.g., BioFire ® FilmArray Blood Culture Identification from Biomerieux in Salt Lake City, UT, USA and Luminex ® VERIGENE ® from DIASORIN in Saluggia, Italy), matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF), or Loop-Mediated Isothermal Amplification (LAMP) based tests performed on a positive blood culture have shown to reduce pathogen detection time and optimize the antibiotic regimen [6][7][8][9]. The disadvantages of these methods are the requirement of a positive blood culture as well as the know-how and availability of the microbiology lab [5].…”

Section: Introductionmentioning

confidence: 99%

Point-of-Care Method T2Bacteria®Panel Enables a More Sensitive and Rapid Diagnosis of Bacterial Blood Stream Infections and a Shorter Time until Targeted Therapy than Blood Culture

Clodi-Seitz,

Baumgartner,

Turner

et al. 2024

Microorganisms

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Background: Rapid diagnosis and identification of pathogens are pivotal for appropriate therapy of blood stream infections. The T2Bacteria®Panel, a culture-independent assay for the detection of Escherichia coli, Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa in blood, was evaluated under real-world conditions as a point-of-care method including patients admitted to the internal medicine ward due to suspected blood stream infection. Methods: Patients were assigned to two groups (standard of care—SOC vs. T2). In the SOC group 2 × 2 blood culture samples were collected, in the T2 group the T2Bacteria®Panel was performed additionally for pathogen identification. Results: A total of 94 patients were included. Pathogens were detected in 19 of 50 patients (38%) in the T2 group compared to 16 of 44 patients (36.4%) in the SOC group. The median time until pathogen detection was significantly shorter in the T2 group (4.5 h vs. 60 h, p < 0.001), as well as the time until targeted therapy (antibiotic with the narrowest spectrum and maximal effectiveness) (6.4 h vs. 42.2 h, p = 0.043). Conclusions: The implementation of the T2Bacteria®Panel for patients with sepsis leads to an earlier targeted antimicrobial therapy resulting in earlier sufficient treatment and decreased excessive usage of broad-spectrum antimicrobials.

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“…One promising technique is the loop‐mediated isothermal amplification PCR (LAMP) Eazyplex® (AmplexDiagnostics GmbH), which allows obtaining a fast result, in ~30 min, including the handling of the sample. Its usefulness has already been proved in the detection of other pathogens or targets, such as bacterial resistance genes or bacterial identification from blood cultures 17,18 . One of the advantages is that it can be used for one sample at a time, and it is easy to perform with few hands‐on‐time, being suitable for 24/7 on‐demand testing.…”

Section: Introductionmentioning

confidence: 99%

“…Its usefulness has already been proved in the detection of other pathogens or targets, such as bacterial resistance genes or bacterial identification from blood cultures. 17,18 One of the advantages is that it can be used for one sample at a time, and it is easy to perform with few hands-on-time, being suitable for 24/7 on-demand testing.…”

mentioning

confidence: 99%

Evaluation of Eazyplex® LAMP test for fast Candida auris direct detection of colonized patients

Hernández Felices,

Tormo Palop,

Salvador García

et al. 2023

Mycoses

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Candida auris is a multidrug‐resistant pathogen yeast that produces nosocomial outbreaks, due to its ability in colonizing the skin, mucous membranes and surfaces. Rapid diagnosis is essential to control its spread. The aim of this study was to compare the Eazyplex® Candida auris kit (AmplexDiagnostics GmbH) for the rapid identification of patients colonized with C. auris, with the reference method used in our institution (culture and identification by MALDI‐TOF). This easy‐to‐perform test allows obtaining a fast result, in ~30 min. First, we achieved a preliminary study from previously characterized Candida species colonies obtained from 51 clinical samples, with 100% agreement between culture isolation and the Eazyplex® Candida auris LAMP. Second, 152 epidemiological surveillance samples (pharyngeal and axillary‐rectal swabs) were tested retrospectively. The sensitivity, specificity, positive and negative predictive values were 91.8%, 98.8%, 98.2% and 94.5%, respectively. Eazyplex® Candida auris showed acceptable results compared with culture in detecting C. auris from surveillance samples with the advantage of single‐test and shorter time for handling and result than culture, in addition to its great specificity, positive and negative predictive values.

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Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures

Cited by 3 publications

References 27 publications

The Evaluation of Eazyplex® SuperBug CRE Assay Usefulness for the Detection of ESBLs and Carbapenemases Genes Directly from Urine Samples and Positive Blood Cultures

The Evaluation of Eazyplex® SuperBug CRE Assay Usefulness for the Detection of ESBLs and Carbapenemases Genes Directly from Urine Samples and Positive Blood Cultures

Point-of-Care Method T2Bacteria®Panel Enables a More Sensitive and Rapid Diagnosis of Bacterial Blood Stream Infections and a Shorter Time until Targeted Therapy than Blood Culture

Evaluation of Eazyplex® LAMP test for fast Candida auris direct detection of colonized patients

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