Performance of the continuous glucose monitoring system (CGMS) during development of ketosis in patients on insulin pump therapy

Abstract

The CGMS device was confirmed to be reliable and accurate during the development of hyperglycaemia and ketotic conditions.

Application of size-exclusion chromatography in the investigation of the in vitro stability of proinsulin and its cleaved metabolites in human serum and plasma

“…Our results agree with recent investigations that reported increased stability of hPI in EDTA plasma and recommended this matrix for measurement of circulating concentrations [3,21]. Moreover some investigators have suggested that in addition to intact hPI, quantification of B-C junctional cleaved metabolites may have diagnostic value [11,12,27,39].…”

“…Accordingly, much effort has gone into developing improved immunoassay methods for quantitative Abbreviations: hPI, human proinsulin; RIA, radioimmunoassay; ELISA, enzymelinked immunosorbant assay; HPLC, high performance liquid chromatography; SEC, size-exclusion chromatography; cpm, counts per min. ଝ This manuscript reviews and summarizes work that was conducted at the determination of circulating concentrations of total, intact peptide [3,[18][19][20] and cleaved metabolites [21,22]. Early RIA methodology tended to detect both intact peptide and cleaved metabolites [23][24][25], while more recent sandwich ELISAs and related immunoanalytical methodologies have permitted the specific detection of intact peptide [3,19,20,21] and cleaved metabolites [22,23,27].…”

“…One important aspect for maximizing the reliability of proinsulin measurements concerns its stability in biological matrices. While some studies have suggested that plasma may be superior to serum [3,21,26], detailed information from a systematic investigation of hPI matrix stability is lacking. In this manuscript, I have reviewed our findings concerning the in vitro stability of hPI in serum and plasma.…”

“…1) and its B-chain-C-peptide cleaved intermediate have gained interest in recent years as potential prognostic biomarkers for pancreatic beta islet cell status [1][2][3], insulin resistance [4][5][6][7], type II diabetes [8][9][10], increased cardiovascular risk in type II diabetes [11][12][13], hypoglycemia [14] and pancreatic tumors and cancer [15][16][17]. Accordingly, much effort has gone into developing improved immunoassay methods for quantitative Abbreviations: hPI, human proinsulin; RIA, radioimmunoassay; ELISA, enzymelinked immunosorbant assay; HPLC, high performance liquid chromatography; SEC, size-exclusion chromatography; cpm, counts per min.…”

Topical problems of diabetes treatment during pregnancy

“…Резкое прекращение подачи инсулина или нарушение его абсорбции немедленно приводят к абсолютному дефициту инсулина в организме и угрожающим жизни метаболическим расстройствам. Всего через два часа после прекращения поступления инсулина из помпы в крови резко повышается содержание глюкозы и 3-гидроксибутирата [22,23]. Риск развития кетоацидоза при ППИИ, обусловленный техническими причинами, можно исключить или значительно снизить.…”