Performance of the commercially available SERION ELISA classic Echinococcus IgG test for the detection of cystic echinococcosis in clinical practice

Sarink, Maarten J.; Koelewijn, Rob; Slingerland, Bibi C. G. C.; Tielens, Aloysius G.M.; Genderen, Perry J.J. van; Hellemond, Jaap J. van

doi:10.1017/s0022149x18000536

Cited by 4 publications

(4 citation statements)

References 18 publications

(29 reference statements)

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“…The dashed line at 15 U/ml represents the cut-off point. E. granulosus specific antibodies were determined with the SERION ELISA classic Echinococcosis IgG test [4]. For two of the three patients the negative as well as the subsequent positive serological results were confirmed by two other methods; a commercially available Indirect Haem Agglutination assay (IHA, Fumouze) and an in-house developed ELISA.…”

Section: Discussionmentioning

confidence: 97%

Ruptured Echinococcus granulosus cysts in migrants: Is excessive antigen release causing false negative serology?

Sarink¹,

Genderen²,

Tielens

et al. 2020

Travel Medicine and Infectious Disease

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Recently, three female patients with a migratory background (Surinam, Afghanistan and Turkey) presented in our hospital with signs of an anaphylactic shock due to a spontaneous Echinococcus granulosus cyst rupture. Although the current literature states that serology for Cystic Echinococcosis (CE) should be positive in these patients [1,2], serology was found to be negative in all three patients at presentation. This prompted us to review the literature, which resulted in a hypothesis that could explain the false negative serology in patients with anaphylaxis due to CE.

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Section: Discussionmentioning

confidence: 97%

Ruptured Echinococcus granulosus cysts in migrants: Is excessive antigen release causing false negative serology?

Sarink¹,

Genderen²,

Tielens

et al. 2020

Travel Medicine and Infectious Disease

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“…11 Newer ELISA techniques have sensitivity and specificity of 98.0% and 96.2%, respectively. 12 Surgery is the treatment of choice. 1 Patients with a preoperative diagnosis of echinococcosis should always receive prophylactic anthelminthic agents (Albendazole 10 mg/kg/ day) for 2 to 4 weeks, which should continue for at least 4 weeks after surgery to reduce the risk of anaphylaxis.…”

Section: Discussionmentioning

confidence: 99%

Pancreatic echinococcosis

Soin

Sharma

Kochar

2019

Baylor University Medical Center Proceedings

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Echinococcosis (hydatid disease) is caused by the larval stage of Echinococcus granulosus. It most frequently involves the liver, followed by the lung. Pancreatic echinococcosis is very rare, even in endemic areas, with an incidence of 0.14% to 0.2%. We describe a case of a 34-year-old woman who presented with epigastric pain for 1 month. Contrast-enhanced computed tomography demonstrated a 4.0-cm hypodense mass in the pancreatic head and an uncinate process with suggestion of hyperdense/enhancing curvilinear densities. Magnetic resonance imaging with and without intravenous contrast showed a nonenhancing cystic mass. Endoscopic ultrasound demonstrated a cystic structure with curvilinear floating membranes consistent with the water lily sign, pathognomonic of hydatid disease. Enzyme-linked immunosorbent assay for echinococcal antigen was positive.KEYWORDS Echinococcosis; hydatid disease; pancreas E chinococcosis is a zoonotic disease caused by the larval stage of Echinococcus parasite. Four different species affect humans; the most common species are E. granulosus (95%), which causes cystic echinococcosis, and E. multilocularis, which causes alveolar echinococcosis. Carnivores are the definitive hosts and herbivores are intermediate hosts in the life cycle of echinococcosis. Humans are inadvertently infected due to ingestion of vegetables contaminated with carnivores' feces. 1 This disease is endemic to the Mediterranean region, Africa, South America, Australia, the Middle East, and India, where stockbreeding and agriculture are common occupations. 2 Hydatid cysts can be seen in any organ, but the most common sites are the liver (50%-77%) and lung (15%-47%), followed by spleen (0.5%-8%) and kidney (2%-4%). Pancreatic hydatids are very rare, even in endemic areas. 3 In the pancreas, the most common location is the pancreatic head (57%), followed by the body (24%) and the tail (19%). 4

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“…The current clinical diagnosis of echinococcosis primarily relies on imaging techniques, and serological test plays a complementary role (McManus et al ., 2012; Sarink et al ., 2018). Imaging diagnosis can distinguish the two medically important CE and AE lesions (Brunetti et al ., 2010), but it fails to discriminate the CE lesions caused by E. granulosus s.s. and E. canadensis , respectively.…”

Section: Discussionmentioning

confidence: 99%

A multiplex PCR for the identification of Echinococcus multilocularis, E. granulosus sensu stricto and E. canadensis that infect human

Chen

Liu

et al. 2019

Parasitology

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Echinococcus granulosus sensu stricto (s.s.), Echinococcus multilocularis and Echinococcus canadensis are the common causes of human echinococcosis in China. An accurate species identification tool for human echinococcosis is needed as the treatments and prognosis are different among species. The present work demonstrates a method for the simultaneous detection of these three Echinococcus species based on multiplex polymerase chain reaction (mPCR). Specific primers of this mPCR were designed based on the mitochondrial genes and determined by extensive tests. The method can successfully detect either separated or mixed target species, and generate expected amplicons of distinct size for each species. Sensitivity of the method was tested by serially diluted DNA, showing a detection threshold as less as 0.32 pg for both E. granulosus s.s. and E. canadensis, and 1.6 pg for E. multilocularis. Specificity assessed against 18 other parasites was found to be 100% except weakly cross-react with E. shiquicus. The assay was additionally applied to 69 echinococcosis patients and 38 healthy persons, confirming the high reliability of the method. Thus, the mPCR described here has high application potential for clinical identification purposes, and can further provide a useful tool for evaluation of serology and imaging method.

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Performance of the commercially available SERION ELISA classic Echinococcus IgG test for the detection of cystic echinococcosis in clinical practice

Cited by 4 publications

References 18 publications

Ruptured Echinococcus granulosus cysts in migrants: Is excessive antigen release causing false negative serology?

Ruptured Echinococcus granulosus cysts in migrants: Is excessive antigen release causing false negative serology?

Pancreatic echinococcosis

A multiplex PCR for the identification of Echinococcus multilocularis, E. granulosus sensu stricto and E. canadensis that infect human

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