Performance of the Check-Direct ESBL Screen for BD MAXTM for detection of asymptomatic faecal carriage of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae

Whole-Genome Sequencing of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli From Human Infections in Finland Revealed Isolates Belonging to Internationally Successful ST131-C1-M27 Subclade but Distinct From Non-human Sources

“…bla CTX–M–55 was identified in two of our isolates, D5 and D18, with different STs (ST1193 and ST59, respectively). This bla gene was additionally identified from two previously sequenced ESBL-producing E. coli isolates from Finland, one from an imported food sample (coriander from Malaysia) representing ST155 ( Kurittu et al, 2021a ) and one from a healthy, human adult fecal sample, representing ST58 ( Gröndahl-Yli-Hannuksela et al, 2020 ). bla CTX–M–55 -harboring E. coli has been reported especially in samples from meat and food-producing animals, as well as in humans in Asian countries ( Zheng et al, 2012 ; Zhang et al, 2014 ; Zeng et al, 2021 ).…”

“…Isolates were also compared to available previously sequenced ESBL/AmpC-producing E. coli isolates from Finland (total n = 67) collected between 2012 and 2018 from broiler meat ( n = 5) ( Päivärinta et al, 2020 ), broiler caecum ( n = 5) ( Päivärinta et al, 2020 ), broiler production including broiler parents ( n = 8) ( Oikarainen et al, 2019 ), egg surfaces ( n = 4) ( Oikarainen et al, 2019 ) and production environment ( n = 1) ( Oikarainen et al, 2019 ), imported food products ( n = 16) ( Kurittu et al, 2021a ), barnacle geese ( n = 9) ( Kurittu et al, 2021b ), wastewater ( n = 1) (unpublished), cattle ( n = 1) ( Päivärinta et al, 2016 ), veterinarians ( n = 9) ( Verkola et al, 2019 ), and healthy adults ( n = 8) ( Gröndahl-Yli-Hannuksela et al, 2020 ; Supplementary Table 2 ). cgMLST-based MST and was constructed with Ridom SeqSphere+ software.…”

“…Carbapenemase-producing E. coli isolates of human origin have been described using WGS in Finland previously ( Räisänen et al, 2020 ), but to the best of our knowledge, publications covering ESBL-producing E. coli isolates from Finland of human clinical origin to a genomic level are scarce. WGS studies on healthy individuals have been conducted in Finland ( Verkola et al, 2019 ; Gröndahl-Yli-Hannuksela et al, 2020 ) and a national surveillance program routinely implements only phenotypic characterization for ESBL-producers ( Räisänen et al, 2019 ). The proportion of ESBL-producing E. coli in blood and urine specimens in patients in Finland has been relatively low, but steadily increasing during recent years ( Räisänen et al, 2019 ).…”

“…The national surveillance has found 7.3% of all blood specimens and 3.1 and 7.2% of urine specimens in women and men, respectively, positive for ESBL-producing E. coli in 2019. A study investigating fecal samples from healthy, adult volunteers in Finland found the prevalence of ESBL-producing E. coli or K. pneumoniae to be 6.3% ( Gröndahl-Yli-Hannuksela et al, 2020 ). A gap in knowledge regarding bla genes and further genomic characterization of ESBL-producing E. coli isolates involved in clinical cases in Finland exists.…”

Five-Hour Detection of Intestinal Colonization with Extended-Spectrum-β-Lactamase-Producing Enterobacteriaceae Using the β-Lacta Phenotypic Test: the BLESSED Study

“…Many diagnostic tests using phenotypic, immunochromatographic, and molecular methods have been developed to detect the presence of ESBL-PE, and several of these tests have shown high sensitivity and specificity ( 12 – 15 ). Molecular biology can be performed directly on rectal samples with good sensitivity and specificity, providing results in only a few hours ( 16 ). However, this method is expensive, requires dedicated equipment, and, thus, may not be affordable for all laboratories.…”