Performance of Surface-Modified Polycaprolactone on Growth Factor Binding, Release, and Proliferation of Smooth Muscle Cells

Darain, Farzana; Chan, Wing Yue; Chian, Kerm Sin

doi:10.1080/1539445x.2010.520797

Cited by 17 publications

(25 citation statements)

References 18 publications

(31 reference statements)

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“…In terms of surface characteristics, ATR-FTIR spectroscopy showed that no additional functional groups were present on the scaffolds after sterilization (Figure 2). In a study by Darain et al, FTIR analysis of PCL films treated with UV/ozone showed a new peak at 1,600 cm -1 , attributed to the formation of -COOH groups [29]. In addition, a large peak at 3,400 cm -1 was observed on treated films, being attributable to -OH groups formed after treatment, which also increased hydrophilicity, an effect we did not observe in this study (not shown).…”

Section: Discussioncontrasting

confidence: 62%

The effect of ozone gas sterilization on the properties and cell compatibility of electrospun polycaprolactone scaffolds

Rediguieri

Bank

Zanin

et al. 2017

Journal of Biomaterials Science, Polymer Edition

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The growing area of tissue engineering has the potential to alleviate the shortage of tissues and organs for transplantation, and electrospun biomaterial scaffolds are extremely promising devices for translating engineered tissues into a clinical setting. However, to be utilized in this capacity, these medical devices need to be sterile. Traditional methods of sterilization are not always suitable for biomaterials, especially as many commonly used biomedical polymers are sensitive to chemical-, thermal- or radiation-induced damage. Therefore, the objective of this study was to evaluate the suitability of ozone gas for sterilizing electrospun scaffolds of polycaprolactone (PCL), a polymer widely utilized in tissue engineering and regenerative medicine applications, by evaluating if scaffolds composed of either nanofibres or microfibres were differently affected by the sterilization method. The sterility, morphology, mechanical properties, physicochemical properties, and response of cells to nanofibrous and microfibrous PCL scaffolds were assessed after ozone gas sterilization. The sterilization process successfully sterilized the scaffolds and preserved most of their initial attributes, except for mechanical properties. However, although the scaffolds became weaker after sterilization, they were still robust enough to use as tissue engineering scaffolds and this treatment increased the proliferation of L929 fibroblasts while maintaining cell viability, suggesting that ozone gas treatment may be a suitable technique for the sterilization of polymer scaffolds which are significantly damaged by other methods.

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Section: Discussioncontrasting

confidence: 62%

The effect of ozone gas sterilization on the properties and cell compatibility of electrospun polycaprolactone scaffolds

Rediguieri

Bank

Zanin

et al. 2017

Journal of Biomaterials Science, Polymer Edition

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“…The carboxyl group is one of the most functional groups for chemical reactions between amino groups from proteins and carboxyl functional groups of the substrate . Graft polymerization of acrylic acid on the polymeric surfaces has been reported in previous studies to introduce carboxylic groups on the surface of scaffolds where acrylic acid acts as a spacer between proteins and the surface .…”

Section: Resultsmentioning

confidence: 90%

“…Darain et al . also investigated the surface modification of PCL membrane using UV–ozone treatment and suggested that the formation of OH and COOH groups on the surface of PCL nanofibers is due to chain scission of PCL backbone and an increase of the end groups on the surface of PCL membrane …”

Section: Resultsmentioning

confidence: 99%

Immobilization of silk fibroin on the surface of PCL nanofibrous scaffolds for tissue engineering applications

Khosravi

Ghasemi‐Mobarakeh

Mollahosseini

et al. 2018

J of Applied Polymer Sci

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Poly(E-caprolactone) (PCL) is explored in tissue engineering (TE) applications due to its biocompatibility, processability, and appropriate mechanical properties. However, its hydrophobic nature and lack of functional groups in its structure are major drawbacks of PCL-based scaffolds limiting appropriate cell adhesion and proliferation. In this study, silk fibroin (SF) was immobilized on the surface of electrospun PCL nanofibers via covalent bonds in order to improve their hydrophilicity. To this end, the surface of PCL nanofibers was activated by ultraviolet (UV)-ozone irradiation followed by carboxylic functional groups immobilization on their surface by their immersion in acrylic acid under UV radiation and final immersion in SF solution. Furthermore, morphological, mechanical, contact angle, and Attenuated total reflection-Fourier transform infrared (ATR-FTIR) were measured to assess the properties of the surfacemodified PCL nanofibers grafted with SF. ATR-FTIR results confirmed the presence of SF on the surface of PCL nanofibers. Moreover, contact angle measurements of the PCL nanofibers grafted with SF showed the contact angle of zero indicating high hydrophilicity of modified nanofibers. In vitro cell culture studies using NIH 3T3 mouse fibroblasts confirmed enhanced cytocompatibility, cell adhesion, and proliferation of the SF-treated PCL nanofibers.Silk fibroin (SF) is a naturally occurring polymer, derived from Bombyx mori silkworm has been used in TE, due to its biocompatibility, low immunoreactivity, biodegradability, suitable oxygen and water vapor permeability, and good mechanical properties. [14][15][16][17][18][19][20] Moreover, SF improves cell adhesion and proliferation for TE applications. 14,16 Combination of the two aforementioned polymers (PCL and SF) can be considered as a synthetic/natural polyblend to

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“…Previously it has been reported that adsorption behavior of biomolecules depends on the type of protein molecules and nature of solid supports [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][13][14][15][16][17][18][19][20][48][49][50][51][52]. In our case dimensions of BSA are 4.7 nm × 4.7 nm × 11 nm, whereas C 60 -FMC adsorbent shows characteristics of bimodal pore size of 4.95 nm and 10-15 nm.…”

Section: The Adsorption Performance Of Fullerene Mesoporous Carbon Mamentioning

confidence: 93%

Nano-Confined Synthesis of Fullerene Mesoporous Carbon (C60-FMC) with Bimodal Pores: XRD, TEM, Structural Properties, NMR, and Protein Immobilization

Wahab¹,

Darain²,

Karim³

et al. 2015

Int J Nanomater Nanotechnol Nanomed

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Nanoconfined synthesized crystalline fullerene mesoporous carbon (C 60 -FMC) with bimodal pore architectures of 4.95 nm and 10-15 nm pore sizes characterized by XRD, TEM, nitrogen adsorption/ desorption isotherm and solid-state NMR, and the material was used for protein immobilization. The solid-state 13 C NMR spectrum of C 60 -FMC along with XRD, BET and TEM confirms the formation of fullerene mesoporous carbon structure C 60 -FMC. The immobilization of albumin (from bovine serum, BSA) protein biomolecule in a buffer solution at pH 4.7 was used to determine the adsorption properties of the C 60 -FMC material and its structural changes investigated by FT-IR. We demonstrated that the C 60 -FMC with high surface area and pore volumes have excellent adsorption capacity towards BSA protein molecule. Protein adsorption experiments clearly showed that the C 60 -FMC with bimodal pore architectures (4.95 nm and 10-15 nm) are suitable material to be used for protein adsorption.and biomolecules or drugs adsorption on mesoporous carbons have been reported in the literatures [13][14][15][16][17][18][19][20]. It is also observed that mesoporous carbon materials exhibited better performance for these applications than that of counterpart silica material. Then, instead of using a conventional carbon source as mentioned above [1][2][3][4][12][13][14][15][16][17][18][19][20] it will be necessary to find a new carbon source to create a new mesoporous carbon solid framework that will improve the adsorption of biomolecules. As such, Fullerenes (C 60 ) as carbon source consists of nanoscopic building block with surface tailorable that can give unique functionalities to the mesoporous carbon framework [21][22][23]. Previously, fullerene C 60 has been partly used along with porous silica for extending their surface functionalities [24][25][26][27][28][29][30][31][32] but C 60 has not been reported to be used as sole carbon source for preparing fullerene mesoporous carbon that can be useful for protein immobilization.With this concept in mind, this paper, successfully demonstrate the use of fullerene C 60 as carbon source to prepare a new class of fullerene mesoporous carbon (C 60 -FMC) with bimodal pore architectures via pore filling method and its properties for protein immobilization. Experimental Section MaterialsTetraethylorthosilicate (TEOS), Pluronic surfactant P123 (EO20PPO70EO20), Fullerene C 60 , Trimethylbenzene (TMB) and Albumin from Bovine serum (BSA) from Sigma-Aldrich were used as received without further purification.

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Performance of Surface-Modified Polycaprolactone on Growth Factor Binding, Release, and Proliferation of Smooth Muscle Cells

Cited by 17 publications

References 18 publications

The effect of ozone gas sterilization on the properties and cell compatibility of electrospun polycaprolactone scaffolds

The effect of ozone gas sterilization on the properties and cell compatibility of electrospun polycaprolactone scaffolds

Immobilization of silk fibroin on the surface of PCL nanofibrous scaffolds for tissue engineering applications

Nano-Confined Synthesis of Fullerene Mesoporous Carbon (C60-FMC) with Bimodal Pores: XRD, TEM, Structural Properties, NMR, and Protein Immobilization

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