Performance of production of polyhydroxyalkanoates from food waste fermentation with Rhodopseudomonas palustris

Dan, Talya Bergstein-Ben; Jing, Huiyan; Tian, Shen; Zhu, Jun; Yan-ping, Liu

doi:10.1016/j.biortech.2023.129165

Cited by 9 publications

(2 citation statements)

References 41 publications

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“…Exceeding the sonication time of 10 min had no significant effect (p > 0.05) on glucose liberation after the enzymatic action. Dan et al successfully hydrolysed campus canteen FW through ultrasonication for 10 min prior to raw material saccharification for durable PHA synthesis via Rhodopseudomonas palustris [45]. Furthermore, a recent investigation has reported remarkable starch and cellulose solubilisation and saccharification using sonication pretreatment before the hydrolysis of enzyme mixtures, yielding 17.16 g/100 g of household FW.…”

Section: Glucose Liberation In Response To Fw Hydrolysismentioning

confidence: 99%

Sustainable Polyhydroxyalkanoate Production from Food Waste via Bacillus mycoides ICRI89: Enhanced 3D Printing with Poly (Methyl Methacrylate) Blend

Rofeal,

Abdelmalek,

Pietrasik

2023

Polymers

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In view of implementing green technologies for bioplastic turning polices, novel durable feedstock for Bacillus mycoides ICRI89 used for efficient polyhydroxybutyrate (PHB) generation is proposed herein. First, two food waste (FW) pretreatment methods were compared, where the ultrasonication approach for 7 min was effective in easing the following enzymatic action. After treatment with a mixture of cellulase/amylases, an impressive 25.3 ± 0.22 g/L of glucose was liberated per 50 g of FW. Furthermore, a notable 2.11 ± 0.06 g/L PHB and 3.56 ± 0.11 g/L cell dry eight (CDW) over 120 h were generated, representing a productivity percentage of 59.3 wt% using 25% FW hydrolysate. The blend of polyhydroxybutyrate/poly (methyl methacrylate) (PHB/PMMA = 1:2) possessed the most satisfactory mechanical properties. For the first time, PHB was chemically crosslinked with PMMA using dicumyl peroxide (DCP), where a concentration of 0.3 wt% had a considerable effect on increasing the mechanical stability of the blend. FTIR analysis confirmed the molecular interaction between PHB and PMMA showing a modest expansion of the C=O stretching vibration at 1725 cm−1. The DCP-PHB/PMMA blend had significant thermal stability and biodegradation profiles comparable to those of the main constituent polymers. More importantly, a 3-Dimetional (3D) filament was successfully extruded with a diameter of 1.75 mm, where no blockages or air bubbles were noticed via SEM. A new PHB/PMMA “key of life” 3D model has been printed with a filling percentage of 60% and a short printing time of 19.2 min. To conclude, high-performance polymeric 3D models have been fabricated to meet the pressing demands for future applications of sustainable polymers.

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Section: Glucose Liberation In Response To Fw Hydrolysismentioning

confidence: 99%

Sustainable Polyhydroxyalkanoate Production from Food Waste via Bacillus mycoides ICRI89: Enhanced 3D Printing with Poly (Methyl Methacrylate) Blend

Rofeal,

Abdelmalek,

Pietrasik

2023

Polymers

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“…PHAs possess a range of properties such as mechanical strength and biocompatibility that make them attractive for various applications and due to their versatility, they can be modified and customized in order to suit specific needs. Thus, the unique properties of PHAs make them a sustainable solution in the quest for greener materials in industries such as packaging, agriculture, and biomedical applications [3].…”

Section: Introductionmentioning

confidence: 99%

Polyhydroxyalkanoates (PHAs) Production from the Liquid Fraction of dried/shredded Food Waste

Papadopoulou,

Filippou,

Bouzani

et al.

Global NEST International Conference on Environmental Science &Amp; Technology

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An enriched culture of Polyhydroxyalkanoates (PHAs)-forming bacteria was developed using the liquid fraction (condensate) which is generated during drying and shredding of food waste. A mixed enriched culture was used for the production of PHAs, in two Draw-Fill Reactors (DFRs). The culture was developed using cyclic limitation by carbon and nitrogen. Urea was used as nitrogen source and a mixture of volatile fatty acids (VFAs), lactic acid, ethanol and glucose, simulating the composition of the condensate as carbon source. Two cycles of experiments were performed aiming to investigate the effect of the organic loading on the yields and composition of the produced PHAs. The results showed that the organic loading did not significantly affect the accumulation capacity of PHAs, with the average yield for DFR-1 being 16±5% g PHAs/g DCW (dry cell weight) and for DFR-2 19±3% g PHAs/g DCW. The HV:HB ratio in DFR-1 was estimated to be (19±4):(81±4), whereas in DFR-2 (26±2):(74±2) indicating that the availability of more odd-VFAs may lead to the production of copolymers with higher HV content i.e. a bioplastic with improved properties.

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Potential of activated sludge-derived mixed microbial culture enriched on acetate to produce polyhydroxyalkanoates from various substrates

Ren,

Inoue,

Ike

2024

J Mater Cycles Waste Manag

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The use of waste activated sludge (WAS) as a biocatalyst to produce polyhydroxyalkanoates (PHA) from waste streams may help promote the beneficial use of WAS for low-carbon, sustainable wastewater treatment. However, it remains unclear which types of substrates can be used for efficient PHA production, and how the PHA production can be maximized. This study aimed to assess the substrate versatility of mixed microbial cultures (MMCs) constructed from WAS by enriching PHA-accumulating bacteria using an aerobic dynamic discharge (ADD) process fed with acetate. Twelve different substrates, including organic acids, saccharides, and alcohols, were selected as the test substrates. In single-batch assays, the highest PHA production (583–680 mg/L) was achieved using butyrate, acetate, and pyruvate. In fed-batch assays, > 30 wt% PHA content was achieved using acetate, butyrate, propionate, lactate, and ethanol, with the highest content (60.3 wt%) using acetate. These results indicate that acetate-fed MMC by the ADD process could efficiently produce PHA from volatile fatty acids, lactate, pyruvate, and ethanol. Polyhydroxybutyrate was preferentially produced from acetate, butyrate, pyruvate, lactate, and ethanol, whereas polyhydroxyvalerate was notably produced from propionate. The results suggest that PHA can be efficiently produced from a wide range of substrates using MMCs enriched on a single substrate.

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Performance of production of polyhydroxyalkanoates from food waste fermentation with Rhodopseudomonas palustris

Cited by 9 publications

References 41 publications

Sustainable Polyhydroxyalkanoate Production from Food Waste via Bacillus mycoides ICRI89: Enhanced 3D Printing with Poly (Methyl Methacrylate) Blend

Sustainable Polyhydroxyalkanoate Production from Food Waste via Bacillus mycoides ICRI89: Enhanced 3D Printing with Poly (Methyl Methacrylate) Blend

Polyhydroxyalkanoates (PHAs) Production from the Liquid Fraction of dried/shredded Food Waste

Potential of activated sludge-derived mixed microbial culture enriched on acetate to produce polyhydroxyalkanoates from various substrates

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