Performance of polymeric materials for solid phase extraction prior to chromatographic analysis

Bohrer, Denise; Santos, Marlei Veiga dos; Ramírez, Adrián; Nascimento, Paulo Cı́cero do; Carvalho, Leandro Machado de

doi:10.1016/j.jchromb.2008.12.025

Cited by 5 publications

(6 citation statements)

References 12 publications

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“…The construction of the C‐CP fiber packed tips remains relatively unchanged from previous publications . C‐CP fibers are packed with an interstitial fraction of ε i = 0.6 in 0.8 mm i.d.…”

Section: Methodsmentioning

confidence: 99%

“…As the fibers are virtually nonporous on the size‐scale of proteins , this allows for very high linear velocity (>50 mm/s) protein separations without penalties typical of van Deemter C ‐term broadening . In terms of SPE, microbore columns can be used as inline desalting columns for ESI‐MS or simply cut into centimeter‐long segments and affixed to standard micropipette tips . In this mode, discrete samples in heavy matrices (buffer, saliva, and urine) can be processed in parallel, with load, wash, and elute steps performed in 1‐min time frames using a table‐top microcentrifuge.…”

Section: Introductionmentioning

confidence: 99%

“…In this mode, discrete samples in heavy matrices (buffer, saliva, and urine) can be processed in parallel, with load, wash, and elute steps performed in 1‐min time frames using a table‐top microcentrifuge. This approach allows for protein determinations via UV‐Vis absorbance, ESI‐MS, and MALDI‐MS with high sensitivity .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Capillary‐channeled polymer (C‐CP) fibers for the rapid extraction of proteins from urine matrices prior to detection with MALDI‐MS

Manard

Jones

Marcus

2015

Proteomics Clinical Apps

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Capillary‐channeled polymer (C‐CP) fibers for the rapid extraction of proteins from urine matrices prior to detection with MALDI‐MS

Manard

Jones

Marcus

2015

Proteomics Clinical Apps

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show abstract

“…To this end, the use of polymer-based stationary phases eliminates this issue. In addition, polymeric supports can be prepared in a large variety of shapes and chemistries [14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of trilobal capillary‐channeled polymer fiber columns with superficially porous and monolithic phases toward reversed‐phase protein separations

Billotto

Marcus

2022

J of Separation Science

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A trilobal capillary‐channeled polymer fiber stationary phase is evaluated for its performance for intact protein separations under reversed‐phase high‐performance liquid chromatography conditions. The separation quality, operational characteristics, and protein dynamic loading capacity on the fiber phases are compared to commercially‐available superficially porous and monolithic columns. The trilobal or “y‐shaped” polypropylene fiber phase was employed to separate a synthetic mixture of five proteins (having diverse chemistries and molecular weights). The separation quality was evaluated based on the resolution, peak heights/recoveries, peak widths, and peak areas. The present work illustrates the unique ability to operate at higher linear velocities (47.5 mm/s) while maintaining lower back pressures (∼4 MPa), faster separation times (<8 min), and faster gradient rates using the fiber columns while yielding comparable chromatographic performance to the commercial columns. The separations employing the commercial stationary phases operate at lower linear velocities (∼3.0 mm/s), higher back pressures (∼9 MPa), require longer separation times (10 min), and require slightly higher compositions of organic mobile phase to effect protein elution. Likewise, based on breakthrough loading analysis of lysozyme and bovine serum albumin, the trilobal, polypropylene C‐CP fiber column stationary phases demonstrate 3–9X greater binding capacities on a bed volume basis versus the commercial columns.

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“…The SPE sorbents are based mainly on modification of silica , copolymers of styrene and divinylbenzene . Mixed‐mode sorbents with either ion‐exchange or alkyl groups are also available.…”

Section: Introductionmentioning

confidence: 99%

Comparison of mouse plasma and brain tissue homogenate sample pretreatment methods prior to high-performance liquid chromatography for a new 1,2,4-triazole derivative with anticonvulsant activity

et al. 2015

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The focus of the study was to develop a bio-analytical assay for a 1,2,4-triazole derivative from plasma and brain tissue homogenate samples. The goal was to compare analytical techniques that facilitate high accuracy with simplified sample processing. In this study, commonly used standard protein precipitation and solid-phase extraction methods utilizing C18 and cartridges of Hybrid technology were compared in terms of their ability for sample pretreatment and removal of biological matrices before high-performance liquid chromatography quantification. Fast classical reversed-phase chromatography on a C18 column paired with selective sample preparation using Hybrid solid-phase extraction technology resulted in the most precise bio-analytical determination of the hydrophobic 1,2,4-triazole derivative in both biological samples studied. The obtained recovery values were above 95% with the coefficient of variation lower than 5%.

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Performance of polymeric materials for solid phase extraction prior to chromatographic analysis

Cited by 5 publications

References 12 publications

Capillary‐channeled polymer (C‐CP) fibers for the rapid extraction of proteins from urine matrices prior to detection with MALDI‐MS

Capillary‐channeled polymer (C‐CP) fibers for the rapid extraction of proteins from urine matrices prior to detection with MALDI‐MS

Comparative analysis of trilobal capillary‐channeled polymer fiber columns with superficially porous and monolithic phases toward reversed‐phase protein separations

Comparison of mouse plasma and brain tissue homogenate sample pretreatment methods prior to high-performance liquid chromatography for a new 1,2,4-triazole derivative with anticonvulsant activity

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