Performance of nanoparticles for biomedical applications: The <i>in vitro</i>/<i>in vivo</i> discrepancy

Berger, Simone; Berger, M.; Bantz, Christoph; Maskos, Michael; Wagner, Ernst

doi:10.1063/5.0073494

Cited by 12 publications

(9 citation statements)

References 339 publications

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“…142,146,147 Moreover, because specific antibodies can be attached to the surface of graphene-based nanoforms, several voltammetric biomarkers have also been constructed, reported to be suitable in medical diagnostics. [148][149][150] From most of the voltammetric studies presented so far, it seems that the future of voltammetry will be closely linked to the further development of current and novel forms of various nanoparticles.…”

Section: Resultsmentioning

confidence: 99%

Future of Voltammetry

Gulaboski

2022

Maced. J. Chem. Chem. Eng.

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It is exactly one hundred years ago when the first paper about the development of polarography was published in 1922. Polarography is considered a predecessor of voltammetry, and this iconic electrochemical technique was designed by the Nobel laureate Jaroslav Heyrovsky. In this short review, the aim is to highlight some of the most important achievements of voltammetry so far. While hints are given to some of the most important theoretical works related to various electrode mechanisms in cyclic voltammetry and pulse voltammetric techniques, a critical part is written that should help to improve the communication between theoretical and experimental electrochemists. Since a main application of voltammetry is in the field of constructing biosensors, some of the major achievements and several drawbacks of applying voltammetric techniques in designing sensors are discussed. In a small part of this review, the role of nanomaterials in voltammetry is also considered. As scanning electrochemical microscopy (SECM) seems to be most promising instrumental system that will bring voltammetry a step closer to probing real biological systems, critical aspects about the weaknesses of this technique are also briefly discussed. In the final outlooks, we present a set of directions in which voltammetry will develop in the coming years. The paper is written in a way to motivate younger electrochemists to get more involved in exploring the voltammetry.

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Section: Resultsmentioning

confidence: 99%

Future of Voltammetry

Gulaboski

2022

Maced. J. Chem. Chem. Eng.

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“…1 Nonviral carriers for the delivery of different nucleic acids, including their main components, particle types as well as shielding and targeting agents for organ-or cell-specific delivery upon systemic injection. Created with BioRender.com nanocarriers, however, are confronted with blood components like plasma proteins that adsorb on particle surface and hence sustainably affect circulation, transport to tissues and cellular uptake [38][39][40]. For instance, the formation of a protein corona comprised of opsonins will mediate phagocytosis removing particles from the circulation.…”

Section: Shieldingmentioning

confidence: 99%

“…Potential reasons for this observation must be considered and evaluated in order to find explanations for this translational bottleneck. There is a great discrepancy between the results obtained from in vitro studies and in vivo performance, making predictions for (pre-)clinical studies questionable when drawn from cell culture evaluation [38][39][40].…”

Section: Active Targeting: In Vitro Versus In Vivomentioning

confidence: 99%

Directing the Way—Receptor and Chemical Targeting Strategies for Nucleic Acid Delivery

Steffens¹,

Wagner

2022

Pharm Res

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Nucleic acid therapeutics have shown great potential for the treatment of numerous diseases, such as genetic disorders, cancer and infections. Moreover, they have been successfully used as vaccines during the COVID-19 pandemic. In order to unfold full therapeutical potential, these nano agents have to overcome several barriers. Therefore, directed transport to specific tissues and cell types remains a central challenge to receive carrier systems with enhanced efficiency and desired biodistribution profiles. Active targeting strategies include receptor-targeting, mediating cellular uptake based on ligand-receptor interactions, and chemical targeting, enabling cell-specific delivery as a consequence of chemically and structurally modified carriers. With a focus on synthetic delivery systems including polyplexes, lipid-based systems such as lipoplexes and lipid nanoparticles, and direct conjugates optimized for various types of nucleic acids (DNA, mRNA, siRNA, miRNA, oligonucleotides), we highlight recent achievements, exemplified by several nucleic acid drugs on the market, and discuss challenges for targeted delivery to different organs such as brain, eye, liver, lung, spleen and muscle in vivo.

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“…In the last decade, accumulated evidence about the importance of the stem cell microenvironment in modeling human tissue in vitro led to a transition from 2D to 3D culture (Caswell and Zech, 2018;Ingber, 2020;Jensen and Teng, 2020;Dudaryeva et al, 2021;Indana et al, 2021;Jacchetti et al, 2021;Berger et al, 2022). As compared to conventional planar models, 3D systems add a new layer of biological relevance by recapitulating 3D tissue complexity and structural organizations, including critical cell-to-cell and cell-matrix interactions (Birgersdotter et al, 2005;Anselme et al, 2018;Jensen and Teng, 2020;Dudaryeva et al, 2021;Yamada et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Customizable 3D printed perfusion bioreactor for the engineering of stem cell microenvironments

Dupard

García²,

Bourgine³

2023

Front. Bioeng. Biotechnol.

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Faithful modeling of tissues and organs requires the development of systems reflecting their dynamic 3D cellular architecture and organization. Current technologies suffer from a lack of design flexibility and complex prototyping, preventing their broad adoption by the scientific community. To make 3D cell culture more available and adaptable we here describe the use of the fused deposition modeling (FDM) technology to rapid-prototype 3D printed perfusion bioreactors. Our 3D printed bioreactors are made of polylactic acid resulting in reusable systems customizable in size and shape. Following design confirmation, our bioreactors were biologically validated for the culture of human mesenchymal stromal cells under perfusion for up to 2 weeks on collagen scaffolds. Microenvironments of various size/volume (6–12 mm in diameter) could be engineered, by modulating the 3D printed bioreactor design. Metabolic assay and confocal microscopy confirmed the homogenous mesenchymal cell distribution throughout the material pores. The resulting human microenvironments were further exploited for the maintenance of human hematopoietic stem cells. Following 1 week of stromal coculture, we report the recapitulation of 3D interactions between the mesenchymal and hematopoietic fractions, associated with a phenotypic expansion of the blood stem cell populations.Our data confirm that perfusion bioreactors fit for cell culture can be generated using a 3D printing technology and exploited for the 3D modeling of complex stem cell systems. Our approach opens the gates for a more faithful investigation of cellular processes in relation to a dynamic 3D microenvironment.

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Performance of nanoparticles for biomedical applications: The in vitro/in vivo discrepancy

Cited by 12 publications

References 339 publications

Future of Voltammetry

Future of Voltammetry

Directing the Way—Receptor and Chemical Targeting Strategies for Nucleic Acid Delivery

Customizable 3D printed perfusion bioreactor for the engineering of stem cell microenvironments

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