Performance of MTBDRplus assay in detecting multidrug resistant tuberculosis at hospital level

Kebede, Abebaw; Demisse, Daniel; Assefa, Meazashwork; Getachew, Zerihun; Yenew, Bazezew; Tedla, Yared; Ameni, Gobena

doi:10.1186/s13104-017-2989-7

Cited by 9 publications

(26 citation statements)

References 16 publications

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“…The sensitivity of GenoType MTBDR plus assay for detection of INH resistance in the present study was lower than those reported by previous studies in Ethiopia, 48 , 51 Uganda 55 and Pakistan. 49 The MTBDR plus assay was not able to detect the INH resistance in the two isolates, which were detected by MGIT 960 system.…”

Section: Discussioncontrasting

confidence: 92%

“… 47 This isolate, in addition, missed WT katG gene and developed katG MUT2 with an amino acid change of S315T2 (AGC→ACA). Similarly, such rpoB gene mutation types were observed at low frequency in studies conducted in Ethiopia 48 and Pakistan, 49 and at high frequency in a study conducted in East Hungary. 50 The amino acid change of S315T2 (AGC→ACA) in this isolate was not previously reported from Ethiopia but the most common reported amino acid change in Ethiopia has been S315T1, which is in the range of 83%–100%.…”

Section: Discussionmentioning

confidence: 81%

“… 47 Such type of mutation with an amino acid change of H526D was previously reported in Ethiopia in the proportion of 0%–22%. 28 , 48 , 51 , 53 The other RIF mono-resistant isolate missed rpoB WT8 and had no known mutation, which may not indicate real resistance but rather it could be due to a silent mutation in rpoB gene. 54 Similarly, such resistance was reported in previous studies of Ethiopia with various proportions including 11.8% 53 and 44.4%.…”

Section: Discussionmentioning

confidence: 99%

“… 50 The amino acid change of S315T2 (AGC→ACA) in this isolate was not previously reported from Ethiopia but the most common reported amino acid change in Ethiopia has been S315T1, which is in the range of 83%–100%. 28 , 48 , 51 – 53 …”

Section: Discussionmentioning

confidence: 99%

“… 51 But most RIF-resistant isolates missing rpoB WT8 are accompanied by an S531L amino acid change. 28 , 48 , 53 , 55 RIF mono-resistant isolates were identified from the new TB cases, which could be acquired from TB patients who had RIF-resistant TB or the patients could previously be treated with RIF for other bacterial infection. 42 , 43 Molecular detection of RIF resistance is used as a surrogate marker for MDR-TB.…”

Section: Discussionmentioning

confidence: 99%

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Phenotypic and genotypic drug sensitivity of <em>Mycobacterium tuberculosis</em> complex isolated from South Omo Zone, Southern Ethiopia

Wondale¹,

Medhin²,

Abebe³

et al. 2018

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BackgroundKnowledge of drug-sensitivity patterns of Mycobacterium tuberculosis complex (MTBC) strains isolated from patients is an important aspect of TB control strategy. This study was conducted to evaluate the drug sensitivity of MTBC isolates in South Omo, southern Ethiopia.Materials and methodsA total of 161 MTBC isolates (153 from new cases and eight re-treatment TB cases) were isolated using Lowenstein Jensen medium of which 126 isolates were able to be tested for drug sensitivity by BACTEC™MGIT™ 960 system, while all the 161 isolates were tested by GenoType® MTBDRplus VER 2.0. Descriptive statistics and logistic regression were used to express and present results.ResultsOn the basis of MGIT 960 system, the prevalence of mono-resistance was 9.2% (11/119) in the new cases, although neither poly-resistance nor multidrug resistance (MDR) was recorded in these cases. On the basis of GenoType MTBDRplus assay, two of the 153 isolates (1.3%) of the new cases were mono-resistant for rifampicin (RIF) and one of these isolates had known rpoB gene mutation (H526D). One of the eight (12.5%) isolates obtained from the re-treatment cases was MDR with rpoB gene mutation (D516V) and katG gene mutation (S315T2). Taking MGIT 960 system as a gold standard, the sensitivities of the MTBDRplus assay were 33.3%, 100% and 100% for detection of resistance to isoniazid, RIF and MDR, respectively. On the other hand, its specificities were 99.2%, 100% and 100% for detection of resistance to RIF, isoniazid and MDR, respectively.ConclusionThe magnitude of drug resistance was relatively low in the new TB cases of South Omo as compared to the reports from the other regions of the country. This is encouraging and hence the TB Control Program in the Zone should strengthen its program so that the emergence of drug resistance is inhibited.

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