Performance of light-emitting diode fluorescence microscope for diagnosis of tuberculosis

Bhalla, Manpreet; Sidiq, Zeeshan; Sharma, Prem Prakash; Singhal, Ritu; Myneedu, V P; Sarin, Rohit

doi:10.1016/j.ijmyco.2013.05.001

Cited by 25 publications

(25 citation statements)

References 8 publications

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“…Although sensitivity might not represent a major advantage over ZN microscopy, the device-associated reduction in laboratory workload [3,14,15,21,23] remains a strong advantage in terms of cost-efficiency. LED-FM specificity is overall high and should not be a barrier to device introduction in high-TB-burden and limited-resource countries.…”

Section: Resultsmentioning

confidence: 99%

“…Seven studies reported provision of training of various length [13,14,16,[19][20][21]23], but training provision did not consistently translate into better LED-FM performance accuracy (data not shown). …”

Section: Subgroup Accuracy Analysismentioning

confidence: 99%

“…12 articles [13][14][15][16][17][18][19][20][21][22][23][24], reporting 12 independent studies, were included in the meta-analysis. One study [15] had two different patient groups (group 1: unlikely HIV-infected; group 2: HIV-infected) with separately analysed data.…”

Section: Study Selection and Characteristicsmentioning

confidence: 99%

“…The 12 prospective studies [13][14][15][16][17][18][19][20][21][22][23][24] were conducted across 11 countries and assessed six commercial LED-FM systems (table 1). All studies were located in middle-or low-income countries [25] and 11 recruited sites in WHO-recognised, high-TB-burden countries [1].…”

Section: Study Selection and Characteristicsmentioning

confidence: 99%

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Light-emitting diode fluorescence microscopy for tuberculosis diagnosis: a meta-analysis

Chang

Page²,

Bonnet³

2015

Eur Respir J

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Light-emitting diode fluorescence microscopy (LED-FM) is recommended by the World Health Organization to replace conventional Ziehl-Neelsen microscopy for pulmonary tuberculosis diagnosis. Uptake of LED-FM has been slow. One reason is its reported loss of specificity compared with Ziehl-Neelsen microscopy. We aimed to determine the diagnostic accuracy of LED-FM for tuberculosis detection and explore potential factors that might affect its performance.A comprehensive search strategy based on pre-specified criteria was employed to identify eligible studies between January 1, 2000 and April 1, 2014 in 11 databases. Standardised study selection, data extraction and quality assessment were conducted. Pooled sensitivity and specificity of LED-FM using culture as the reference standard were estimated through meta-analyses using a bivariate random-effects model. Investigation of heterogeneity was performed by subgroup analyses.We identified 12 unique studies, half of which were from peripheral healthcare facilities. LED-FM achieved a pooled sensitivity of 66.9% (95% CI 60.5-72.7%) and pooled specificity of 96.8% (95% CI 93.1-98.6%). A pooled sensitivity of 53.0% (95% CI 42.8-63.0%) and pooled specificity of 96.1% (95% CI 86.0-99.0%) were obtained by LED-FM among HIV-infected patients. Study methodology factors and differences in the LED-FM procedure or device could also affect the performance.LED-FM specificity is high and should not be a barrier to device introduction, particularly among peripheral healthcare settings where this technology is meant to be used. Sensitivity is reduced in HIVinfected patients. @ERSpublications Meta-analysis showed high performance of LED fluorescence microscopy in diagnosing pulmonary TB http://ow.ly/U3dxd

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Section: Resultsmentioning

confidence: 99%

Section: Subgroup Accuracy Analysismentioning

confidence: 99%

Section: Study Selection and Characteristicsmentioning

confidence: 99%

Section: Study Selection and Characteristicsmentioning

confidence: 99%

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Light-emitting diode fluorescence microscopy for tuberculosis diagnosis: a meta-analysis

Chang

Page²,

Bonnet³

2015

Eur Respir J

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“…These specimens were collected and packed with ice gel pack and transported through courier as per standard operating procedure from DTC's to the laboratory. The smear microscopy was performed by fluorescent microscopy method (Auromine-O)-light emitting diode FM [22]. Based on smear microscopy results, the smear positive specimens 4897 were subjected to LPA genotypic method.…”

Section: Specimens and The Study Populationmentioning

confidence: 99%

Mutations in the Rpob Gene of Mycobacterium Tuberculosis Identified by Sequencing Method

Ramalingam

Murali²,

Palaniswamy³

2017

Asian J Pharm Clin Res

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Objective: To identify the mutation in the rpoB gene of Mycobacterium tuberculosis (MTB), using by sequencing method from pulmonary specimens of presumptive TB patients belonging to the districts of Tamil Nadu.Methods: A total of 8697 clinical specimens of presumptive MTB patients were collected from various districts of Tamil Nadu. Smear microscopy was performed by light emitting diode fluorescent microscopy and all the smear positive samples were tested using line probe assay (LPA) to detect the percentage of drug resistance pattern and to identify the missing mutation in LPA by the sequencing method.Results: Among 4897 smear positives subjected to LPA method; 407 (8.3%) MTB was not detected and 16 (0.3%) showed invalid result; 4473 (91.4%) strains showed MTB positive; 3695 (82.6%) were sensitive for both rifampicin (RIF) and isoniazid (INH) drugs; 502 (11.2%) were resistance for INH; 73 (1.6%) resistant for RIF; 203 (4.5%) were resistance for RIF and INH. Totally, 52 (1.2%) strains results cannot be confirmed by LPA and reported as sensitive for RIF, because of the faint and the missing bands in both wild type and mutation. These strains were sequenced and 39 (75%) strains showed resistant to RIF. Conclusion:Hence LPA may be the molecular technology for the rapid, feasible and reliable method for the detection of multidrug resistant mutation but few confusion bands cannot be reported as resistance, which should be confirmed by either conventional phenotypic drug susceptibility testing or by sequencing method.

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Automatic Detection of Tuberculosis Bacilli from Microscopic Sputum Smear Images Using Faster R-CNN, Transfer Learning and Augmentation

El-Melegy

Mohamed

ElMelegy

2019

Lecture Notes in Computer Science

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Performance of light-emitting diode fluorescence microscope for diagnosis of tuberculosis

Abstract: Although the use of LED-FM only marginally increased sensitivity, the considerable time saving ability combined with very good acceptance and ease of use makes it a reliable alternative to other conventional methods available.

Cited by 25 publications

References 8 publications

Light-emitting diode fluorescence microscopy for tuberculosis diagnosis: a meta-analysis

Light-emitting diode fluorescence microscopy for tuberculosis diagnosis: a meta-analysis

Mutations in the Rpob Gene of Mycobacterium Tuberculosis Identified by Sequencing Method

Automatic Detection of Tuberculosis Bacilli from Microscopic Sputum Smear Images Using Faster R-CNN, Transfer Learning and Augmentation

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