Performance of Latex agglutination, ELISA and RT-PCR for diagnosis of Rotavirus infection

Hamzavi, Hosna; Azaran, Azarakhsh; Makvandi, Manoochehr; Karami, Sahar; Ardakani, Mohammad Roayaei; Nejad, Amir Sasan Mozaffari

doi:10.4081/jbr.2017.6522

Cited by 4 publications

(4 citation statements)

References 12 publications

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“…The discrepancy of these methods has been observed previously ( 22 ). Most studies report a higher percentage for the LAT test and the highest specificity for the RT-PCR ( 23 ), whilst in our study LAT positive results were all confirmed by viral genome detection, resulting in a very high specificity.…”

Section: Discussionsupporting

confidence: 62%

High Occurrence Among Calves and Close Phylogenetic Relationships With Human Viruses Warrants Close Surveillance of Rotaviruses in Kuwaiti Dairy Farms

et al. 2022

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Rotavirus, one of the main pathogens causing morbidity and mortality in neonatal dairy calves worldwide, is responsible for 30–44% of cattle deaths. It is considered to be the most common etiologic agent of diarrhea in neonatal dairy calves and children, the dominant type being group A. Two hundred seventy animals from 27 farms from 2 regions of Kuwait were tested for the presence of Rotavirus serogroup A (RVA) using latex agglutination test (LAT) and reverse transcription–polymerase chain (RT-PCR) testing. RVA non-structural proteins NSP1-2, NSP4-5 and capsid protein genes VP1-7 were characterized by next generation sequencing. LAT was positive in 15.56% of the animals, and RT-PCR in 28.89%. Using RT-PCR as a reference method, LAT was 100% specific but only 83.33% sensitive. ANOVA analysis showed correlation only with the location of the farms but no significant correlation with the age and sex of the animals. Although there was a tendency of clustering of RVA positive animals, it did not reach statistical significance (p = 0.035 for LAT). The phylogenetic analysis showed that Kuwaiti isolates of group A rotavirus clustered with human rotaviruses. Taken together, it seems that rotavirus was present in most of the dairy farms in Kuwait. The high occurrence of the virus in calves in Kuwaiti dairy farms and the close phylogenetic affinity with human isolates warrants urgent action to minimize and control its spread between calves in farms.

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Section: Discussionsupporting

confidence: 62%

High Occurrence Among Calves and Close Phylogenetic Relationships With Human Viruses Warrants Close Surveillance of Rotaviruses in Kuwaiti Dairy Farms

et al. 2022

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“…Group A rotavirus was the second most prevalent virus detected in our study, accounting for 24%. Two Korean studies reported similar detection rates of 24.8% and 26.9% for rotavirus using the same kit [21]; in Iran, rotavirus was detected in 27.0% of cases using RT-PCR [22]. However, higher results were reported in three other studies using RT-PCR for the detection of viruses causing gastroenteritis in Egyptian children, in which detection rates of rotavirus were 37.0%, 39.0% and 57.4%, respectively [13,20,23].…”

Section: Discussionmentioning

confidence: 96%

Use of multiplex PCR in diagnosis of childhood acute viral diarrhoea caused by rotavirus, norovirus, astrovirus and adenovirus in Upper Egypt

Othma

Gomaa

Anany

et al. 2022

Egypt J Med Hum Genet

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Background Diarrhoea is still a major public health issue in developing countries, and it is one of the leading causes of morbidity and mortality in children. We aimed to assess the use of a multiplex reverse transcription polymerase chain reaction (RT-PCR) assay for the detection of five viruses, including rotavirus, norovirus (genogroups 1 and 2), astrovirus, and adenovirus, responsible for gastroenteritis in children under 5 years old in primary care centres in Upper Egypt. Subjects and methods A total of 500 stool samples were collected. Fifty samples were randomly selected for viral examination using multiplex RT-PCR for the detection of rotavirus, norovirus (genogroups 1 and 2), astrovirus, and adenovirus, causing diarrhoea. Results Viruses were detected in 45 (90%) of the 50 stool samples. The most frequently identified virus was norovirus G2, followed by Group A rotavirus, astrovirus and adenovirus. Mixed infection by two and three viruses was observed in 7/50 cases (14%) and 2/50 cases (4%), respectively. Norovirus G1 was not detected in the samples examined. Conclusion Our study reveals that multiplex PCR allows for the detection of multiple viral targets in only one reaction, rendering the assay easier to perform compared to existing testing methodologies (RT-PCR and electron microscopy). Additionally, most of the viruses were detected in summer, and the highest prevalence was in the age group less than 1 year. Norovirus G2 and rotavirus were the most frequent agents and the most common coinfections responsible for gastroenteritis in children.

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“…Norovirus is one of the predominant causes of acute gastroenteritis worldwide, with approximately 699 million people infected annually ( Ahmed et al, 2014 ; Van Trang et al, 2014 ; Hamzavi et al, 2018 ). Norovirus belongs to the family Caliciviridae and is a single-stranded positive-sense RNA virus with a gene length of approximately 7.5 kb.…”

Section: Introductionmentioning

confidence: 99%

Development of a rapid and accurate CRISPR/Cas13-based diagnostic test for GII.4 norovirus infection

et al. 2022

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Genogroup II genotype 4 (GII.4) norovirus causes acute gastroenteritis in children, and its infection is more severe than that of other genotypes. Early and precise detection and treatment are critical for controlling its spread and reducing the severity of infection. In this study, a rapid and efficient isothermal assay for the GII.4 norovirus detection (GII.4-CRISPR detection) was developed based on the CRISPR/Cas13a system. The assay can be applied without expensive instrumentation, and the results can be read via both fluorescence and lateral flow strip (LFS). The analytical sensitivity of this assay was 5 copies/reaction, and there was no cross-reaction with other genotypes of norovirus or other clinically common pathogens. There was a coincidence rate of 100% between our assay and commercial quantitative polymerase chain reaction. GII.4-CRISPR detection improves upon the shortcomings of some previously established molecular methods of detection, particularly with regard to accessibility. It provides an alternative tool for outbreak control and early diagnosis of GII.4 norovirus infection.

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Performance of Latex agglutination, ELISA and RT-PCR for diagnosis of Rotavirus infection

Cited by 4 publications

References 12 publications

High Occurrence Among Calves and Close Phylogenetic Relationships With Human Viruses Warrants Close Surveillance of Rotaviruses in Kuwaiti Dairy Farms

High Occurrence Among Calves and Close Phylogenetic Relationships With Human Viruses Warrants Close Surveillance of Rotaviruses in Kuwaiti Dairy Farms

Use of multiplex PCR in diagnosis of childhood acute viral diarrhoea caused by rotavirus, norovirus, astrovirus and adenovirus in Upper Egypt

Development of a rapid and accurate CRISPR/Cas13-based diagnostic test for GII.4 norovirus infection

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