Performance of glucose/O2 enzymatic fuel cell based on supporting electrodes over-coated by polymer-nanogold particle composite with entrapped enzymes

Huo, Wen Shan; Zeng, Han; Yang, Yaru; Zhang, Y.H.

doi:10.1016/j.cplett.2017.01.005

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…Some distortions in the micro-environment of enzyme cofactors can be observed, resulting in catalytic activity reduction. Suitable modification of aromatic heterocyclic groups or aromatic rings on the interface of nano-gold particles can favor bio-molecules linkage on nanoparticles surface and support DET mechanism inside the electrode [30]. On the other hand, carbon nanotubes and other nanostructured carbon materials have also been used to decrease the overpotential for NADH (reduced form of NAD) oxidation.…”

Section: Electrode Materials Engineeringmentioning

confidence: 99%

Enzymatic Biofuel Cells: A Review on Flow Designs

et al. 2021

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Because of environmental concerns, there is a growing interest in new ways to produce green energy. Among the several studied applications, enzymatic biofuel cells can be considered as a promising solution to generate electricity from biological catalytic reactions. Indeed, enzymes show very good results as biocatalysts thanks to their excellent intrinsic properties, such as specificity toward substrate, high catalytic activity with low overvoltage for substrate conversion, mild operating conditions like ambient temperature and near-neutral pH. Furthermore, enzymes present low cost, renewability and biodegradability. The wide range of applications moves from miniaturized portable electronic equipment and sensors to integrated lab-on-chip power supplies, advanced in vivo diagnostic medical devices to wearable devices. Nevertheless, enzymatic biofuel cells show great concerns in terms of long-term stability and high power output nowadays, highlighting that this particular technology is still at early stage of development. The main aim of this review concerns the performance assessment of enzymatic biofuel cells based on flow designs, considered to be of great interest for powering biosensors and wearable devices. Different enzymatic flow cell designs are presented and analyzed highlighting the achieved performances in terms of power output and long-term stability and emphasizing new promising fabrication methods both for electrodes and cells.

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Section: Electrode Materials Engineeringmentioning

confidence: 99%

Enzymatic Biofuel Cells: A Review on Flow Designs

et al. 2021

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“…It was also of practical significance to study A in the applications where S t was fixed, especially in the applications of enzymatic electrochemistry such as enzymatic biofuel cells, electrochemical biosensors, and enzymatic electrosynthesis where AuNPs were used to improve the efficiency of electron transfer and enhance the adsorption between enzymes and the electrode. − …”

Section: Introductionmentioning

confidence: 99%

New Insights on the Size-Dependent Inhibition of Enzymes by Gold Nanoparticles

Chen

et al. 2023

Langmuir

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Particle size might affect the inhibition behaviors of gold nanoparticles (AuNPs) on enzyme activity by influencing the density of binding sites (ρ), the association constant (K a), the steric hindrance of enzymes by AuNPs, the binding orientations of the enzyme on AuNPs, as well as the structural changes of enzymes. In previous studies, the effects of the above-mentioned factors, which could not be ignored in the applications of enzymatic electrochemistry, were often overshadowed by the effects of surface area. In order to study the size effect on the inhibition types and inhibitory ability of enzymes by AuNPs, we investigated the inhibition behaviors of chymotrypsin (ChT) by AuNPs with three different sizes (D1-AuNCs, D3-AuNPs, and D6-AuNPs) under the same surface area concentration. The results showed that both of the inhibition types and the inhibition ability varied with the particle size of AuNPs. D1-AuNCs inhibited ChT noncompetitively, while D3/D6-AuNPs inhibited ChT competitively. Contrary to the common sense, D6-AuNPs showed a weaker inhibitory ability than D3-AuNPs. By means of zeta potential, agarose gel electrophoresis, isothermal titration calorimetry, synchronous fluorescence spectroscopy, and circular dichroism, the mechanism of the weak inhibitory ability of D6-AuNPs was found to be the standing binding orientation caused by the small curvature. This work had certain guiding significance for the biosafety of AuNPs, the development of nanoinhibitors, as well as the applications of AuNPs in enzymatic electrochemistry.

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“…GNs-modified electrodes have a large surface area that allows higher enzyme loading, better orientation and more efficient electrical contact [14], excellent chemical stability and resistance to surface poisoning during electrochemical processes, high conductivity, catalytic efficiency, and biocompatibility [15]. Due to these properties, GNs-modified electrodes enhance the electrocatalytic activity of enzymes [16], sometimes provide direct electron transfer [17], and improve the performance of biofuel cells [18,19].…”

Section: Introductionmentioning

confidence: 99%

The Impact of Glucose Oxidase Immobilization on Dendritic Gold Nanostructures on the Performance of Glucose Biosensors

Sakalauskiene

Popov

Kausaitė-Minkstimienė

et al. 2022

Biosensors

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In recent years, many efforts have been made to develop rapid, sensitive and user-friendly glucose biosensors for monitoring blood glucose concentration in patients. In this study, the electrochemical glucose biosensors based on graphite rod (GR) electrode electrochemically modified with dendritic gold nanostructures (DGNs) and glucose oxidase (GOx) were developed. Phenazine methosulfate was used as a soluble redox mediator. Three GOx immobilization methods: adsorption on DGNs and cross-linking with glutaraldehyde (GA) vapour (GA-GOx/DGNs/GR), covalent immobilization on DGNs modified with 11-mercaptoundecanoic acid self-assembled monolayer (SAM) (GOx-SAM/DGNs/GR) and covalent immobilization on SAM with additional cross-linking with GA vapour (GA-GOx-SAM/DGNs/GR), were used. It was determined that GA significantly improved the stability of the enzyme layer. The difference of maximal current generated during the enzymatic reaction (ΔImax) equal to 272.06 ± 8.69 µA was obtained using a biosensor based on GA-GOx/DGNs/GR electrodes. However, the highest ΔImax equal to 384.20 ± 16.06 µA was obtained using GA-GOx-SAM/DGNs/GR electrode. ΔImax for biosensors based on the GA-GOx-SAM/DGNs/GR electrode was 1.41 times higher than for the GA-GOx/DGNs/GR, whereas the linear dynamic range from 0.1 to 10 mM was the same using all three GOx immobilization methods. The limit of detection using GA-GOx-SAM/DGNs/GR and GA-GOx/DGNs/GR electrodes was 0.019 and 0.022 mM, respectively. The ability to detect glucose in the serum by developed biosensors was evaluated.

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Performance of glucose/O2 enzymatic fuel cell based on supporting electrodes over-coated by polymer-nanogold particle composite with entrapped enzymes

Cited by 6 publications

References 21 publications

Enzymatic Biofuel Cells: A Review on Flow Designs

Enzymatic Biofuel Cells: A Review on Flow Designs

New Insights on the Size-Dependent Inhibition of Enzymes by Gold Nanoparticles

The Impact of Glucose Oxidase Immobilization on Dendritic Gold Nanostructures on the Performance of Glucose Biosensors

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