Performance of four diagnostic assays for detecting herpes simplex virus type 2 antibodies in the Middle East and North Africa

Nasrallah, Gheyath K.; Dargham, Soha R.; Sahara, Afifah; Elsidiq, Malaz S.; Abu‐Raddad, Laith J.

doi:10.1016/j.jcv.2019.01.001

Cited by 14 publications

(13 citation statements)

References 34 publications

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“…Test of significance was set at p < .05. Sensitivity, specificity, positive predictive value, negative predictive value, positive percent agreement, and Cohen Kappa were calculated to determine the performance characteristics of each assay with the HIV positives in a polymerase chain reaction, as previously described 28–30 …”

Section: Methodsmentioning

confidence: 99%

New infections and HIV‐1 subtypes among febrile persons and blood donors in Oyo State, Nigeria

Olusola

Olaleye

Odaibo

2021

Journal of Medical Virology

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Purpose There were approximately 37.9 million persons infected with HIV in 2018 globally, resulting in 770,000 deaths annually. Over 50% of this infection and deaths occur in sub‐Saharan Africa, with countries like Nigeria being seriously affected. Nigeria has one of the highest rates of new infections globally. To control HIV infection in Nigeria, there is a need to continually screen high‐risk groups for early HIV infection and subtypes using very sensitive methods. In this study, new HIV‐1 infection and circulating HIV‐1 subtypes among febrile persons and blood donors were determined. Performance characteristics of three commercial EIA kits were also evaluated. Methods In total, 1028 participants were recruited for the study. New HIV‐1 infection and subtypes were determined using enzyme immunoassays and molecular techniques, respectively. Sensitivity, specificity, predictive values, and agreements were compared among the EIA kits using PCR‐confirmed HIV‐positive and negative samples. Results The overall prevalence of HIV infection in this study was 5.35%. The rate of new HIV infection was significantly different (p < .03674) among 1028 febrile persons (Ibadan: 2.22%; Saki: 1.36%) and blood donors (5.07%) studied. Three subtypes, CRF02_AG, A, and G, were found among those with new HIV infection. Whereas the commercial ELISA kits had very high specificities (94.12%, 100%, and 100%) for HIV‐1 detection, Alere Determine HIV‐1 antibody rapid kit had the lowest sensitivity score (50%). Conclusion Genetic diversity of HIV‐1 strains among infected individuals in Oyo State, Nigeria, is still relatively high. This high level of diversity of HIV‐1 strains may impact the reliability of diagnosis of the virus in Nigeria and other African countries where many of the virus strains co‐circulate.

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Section: Methodsmentioning

confidence: 99%

New infections and HIV‐1 subtypes among febrile persons and blood donors in Oyo State, Nigeria

Olusola

Olaleye

Odaibo

2021

Journal of Medical Virology

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“…Blood specimens were collected from 394 volunteering individuals between July 26 and September 9, 2020, as a sub-study of a nationwide survey [8] assessing SARS-CoV-2 seroprevalence (IgG antibodies) and current-infection prevalence (using polymerase chain reaction [PCR] testing) in the wider population of craft and manual workers who constitute 60% of the population of Qatar [11]. Informed by prior work [12, 13], a sample size of 400 was estimated to be sufficient to ensure narrow confidence intervals for the Cohen’s kappa statistic, but we were able to include and test only 394 specimens. The research work was approved by the ethics review boards at HMC, QU, and Weill Cornell Medicine-Qatar.…”

Section: Methodsmentioning

confidence: 99%

Are commercial antibody assays substantially underestimating SARS-CoV-2 ever infection? An analysis on a population-based sample in a high exposure setting

Nasrallah

Dargham

Shurrab

et al. 2020

Preprint

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BackgroundPerformance of three automated commercial serological IgG-based assays was investigated for assessing SARS-CoV-2 ever (past or current) infection in a population-based sample in a high exposure setting.MethodsPCR and serological testing was performed on 394 individuals.ResultsSARS-CoV-2-IgG seroprevalence was 42.9% (95% CI 38.1%-47.8%), 40.6% (95% CI 35.9%-45.5%), and 42.4% (95% CI 37.6%-47.3%) using the CL-900i, VidasIII, and Elecsys assays, respectively. Between the three assays, overall, positive, and negative percent agreements ranged between 93.2%-95.7%, 89.3%-92.8%, and 93.8%-97.8%, respectively; Cohen kappa statistic ranged from 0.86-0.91; and 35 specimens (8.9%) showed discordant results. Among all individuals, 12.5% (95% CI 9.6%-16.1%) had current infection, as assessed by PCR. Of these, only 34.7% (95% CI 22.9%-48.7%) were seropositive by at least one assay. A total of 216 individuals (54.8%; 95% CI 49.9%-59.7%) had evidence of ever infection using antibody testing and/or PCR during or prior to this study. Of these, only 78.2%, 74.1%, and 77.3% were seropositive in the CL-900i, VidasIII, and Elecsys assays, respectively.ConclusionsAll three assays had comparable performance and excellent agreement, but missed at least 20% of individuals with past or current infection. Commercial antibody assays can substantially underestimate ever infection, more so when infection rates are high.

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“…Laboratory analysis methods have been described previously (18,19,28,29). Briefly, for HSV-1 serology testing, we used the HerpeSelect® 1 enzyme linked immunosorbent assay (ELISA) kit (Cat.…”

Section: Specimen Testingmentioning

confidence: 99%

Seroprevalence of Herpes simplex virus types 1 and 2 in Indian and Filipino migrant populations in Qatar: a cross-sectional survey

Nasrallah¹,

Dargham²,

Harfouche³

et al. 2020

East Mediterr Health J

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Background: The epidemiology of herpes simplex virus infections is of growing interest but information on its seroprevalence in many countries is scarce. Aims: This study aimed to measure the seroprevalence of herpes simplex virus type 1 and type 2 in Filipino and Indian men living in Qatar. Methods: Blood serum specimens were collected from male blood donors aged ≥ 18 years in Qatar from 2013 to 2016. HerpeSelect® 1/2 and Euroline-WB assays were used to measure antibodies to herpes simplex virus types 1 and 2 in 120 Filipino and 325 Indian men. Results: The seroprevalence of herpes simplex virus-1 was 84.9% (95% confidence interval (CI): 78.4-90.0%) in Filipino men and 48.3% (95% CI: 43.6-53.0%) in Indian men. The seroprevalence of herpes simplex virus-2 was 8.3% (95% CI: 4.6-13.7%) in Filipinos and 3.7% (95% CI: 2.2-5.9%) in Indians. The seroprevalence of herpes simplex virus types 1 and 2 increased with age, but this trend was only statistically significant in Indian men (P = 0.013 and P = 0.011 respectively). Conclusions: The seroprevalence rates of herpes simplex virus-2 in Filipino and Indian men living in Qatar were similar to those found in the Philippines and India. However, the seroprevalence of herpes simplex virus-1 in Indians, while similar to that found in India, was substantially lower than that of other countries in Asia and developing countries worldwide, which needs further investigation.

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Performance of four diagnostic assays for detecting herpes simplex virus type 2 antibodies in the Middle East and North Africa

Cited by 14 publications

References 34 publications

New infections and HIV‐1 subtypes among febrile persons and blood donors in Oyo State, Nigeria

New infections and HIV‐1 subtypes among febrile persons and blood donors in Oyo State, Nigeria

Are commercial antibody assays substantially underestimating SARS-CoV-2 ever infection? An analysis on a population-based sample in a high exposure setting

Seroprevalence of Herpes simplex virus types 1 and 2 in Indian and Filipino migrant populations in Qatar: a cross-sectional survey

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