Performance of Focus ELISA Tests for HSV-1 and HSV-2 Antibodies Among University Students With No History of Genital Herpes

Mark, Hayley; Nanda, Joy P.; Roberts, J; Rompalo, Anne; Melendez, Johan H.; Zenilman, Jonathan M.

doi:10.1097/01.olq.0000258307.18831.f0

Cited by 32 publications

(19 citation statements)

References 23 publications

(18 reference statements)

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“…The differences in specificity results between our data and Hogrefe et al 4 may be related to populations studied as no other explanatory factor has been identified. It is worth noting that although the specificity of the HerpeSelect ELISA has been found to be relatively low among populations with a low HSV-2 seroprevalence (<5%) in the United States,10 or by geographical location (for example, Vietnam11), the specificity seen here in Kisumu is notably lower and may be specific to certain geographical areas of Africa.…”

Section: Discussionmentioning

confidence: 63%

Herpes simplex virus type 2 antibody detection performance in Kisumu, Kenya, using the Herpeselect ELISA, Kalon ELISA, Western blot and inhibition testing

Smith

Bailey

Westreich

et al. 2008

Sexually Transmitted Infections

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Section: Discussionmentioning

confidence: 63%

Herpes simplex virus type 2 antibody detection performance in Kisumu, Kenya, using the Herpeselect ELISA, Kalon ELISA, Western blot and inhibition testing

Smith

Bailey

Westreich

et al. 2008

Sexually Transmitted Infections

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“…Similarly, studies conducted in South West of Iran showed that the HSV-1 sero-prevalence (79.2%) was higher than HSV-2 (23.3%) (Tayyebi and Sharifi, 2010). In a study conducted in Maryland, Mark et al (2007) obtained an HSV-1 and HSV-2 sero-prevalence of 48 and 3.4% respectively by Western Blot assay. Epidemiologic studies conducted among miners and commercial sex workers in South Africa reported sero-prevalence rates of 60-90% respectively (Auvert et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of HSV1 and HSV2 among HIV Patients in Northern South Africa as Determined by Real Time PCR in Urine Samples

Samie¹,

Mnisi²,

Ramantswana³

2017

American Journal of Infectious Diseases

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The present study determined the prevalence of HSV-1 and 2 among HIV patients in Northern South Africa and identified potential risk factors. Urine samples were collected from patients attending different HIV clinics in Limpopo Province. Total genomic DNA was isolated from these samples using GenElute Blood Genomic DNA Kit and real time PCR protocols were used for the detection of HSV1 and HSV2. Demographic, clinical and socioeconomic data were collected using a structured questionnaire. The overall prevalence of HSV1 was 48.5% while that of HSV2 was 10.8% from a total of 308 samples. The prevalence of HSV1 was higher among females while that of HSV2 did not vary between males and females. Patients on zidovudine had a much lower prevalence. Patients who had TB (χ 2 = 9.128; p = 0.003), seemed to have a much higher susceptibility to HSV-1.However, this was not the case with HSV2. CD4 count and viral load did not seem to have any impact of the occurrence of HSV among these patients. Urine samples appear to be a useful alternative for the detection of the HSV. Generally, history of TB, usage of certain ARVs and early age of sexual debut appeared to be important risk factors for HSV.

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“…There is a debate about the accuracy of the Focus Diagnostics HerpeSelect HSV-2 IgG ELISA test kits used in this study. Some researchers have suggested that the cutoff value for a positive test should be raised from 1.1 to 3.0 or 3.5 when the test is used in low prevalence settings [24,25]. Raising the cutoff value to 3.5 would have affected the study results since 14 of the 32 positive tests fell in the disputed range, decreasing the estimated prevalence from 6.6% to 4.7%.…”

Section: Discussionmentioning

confidence: 99%

The Epidemiology of Herpes Simplex Virus Type-2 Infection Among Pregnant Women in Rural Mysore Taluk, India

Madhivanan

Bochner

Krupp

et al. 2010

Annals of Epidemiology

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Objectives. To assess the prevalence and determinants of herpes simplex virus type 2 (HSV-2) infections among pregnant women attending mobile antenatal health clinic in rural villages in Mysore Taluk, India. Methods. Between January and September 2009, 487 women from 52 villages participated in this study. Each participant consented to provide a blood sample for HSV-2 and HIV testing and underwent an interviewer-administered questionnaire. Results. HSV-2 prevalence was 6.7% (95% confidence interval (CI) 4.4-9.0), and one woman tested positive for HIV. The median age of women was 20 years and 99% of women reported having a single lifetime sex partner. Women whose sex partner traveled away from home had 2.68 (CI: 1.13-6.34) times the odds of being HSV-2 seropositive compared to women whose sex partner did not travel. Having experienced genital lesions was also associated with HSV-2 infection ( value = 0.08). Conclusion. The 6.7% HSV-2 prevalence was similar to results obtained in studies among pregnant women in other parts of India. It appeared that most women in this study contracted HSV-2 from their spouses and few regularly used condoms. This finding highlights the need for public health policies to increase awareness and education about prevention methods among women and men living in rural India.

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Performance of Focus ELISA Tests for HSV-1 and HSV-2 Antibodies Among University Students With No History of Genital Herpes

Cited by 32 publications

References 23 publications

Herpes simplex virus type 2 antibody detection performance in Kisumu, Kenya, using the Herpeselect ELISA, Kalon ELISA, Western blot and inhibition testing

Herpes simplex virus type 2 antibody detection performance in Kisumu, Kenya, using the Herpeselect ELISA, Kalon ELISA, Western blot and inhibition testing

Prevalence of HSV1 and HSV2 among HIV Patients in Northern South Africa as Determined by Real Time PCR in Urine Samples

The Epidemiology of Herpes Simplex Virus Type-2 Infection Among Pregnant Women in Rural Mysore Taluk, India

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