Performance of electrochemical immunoassays for clinical diagnostics of SARS-CoV-2 based on selective nucleocapsid N protein detection: Boron-doped diamond, gold and glassy carbon evaluation

Białobrzeska, Wioleta; Ficek, Mateusz; Dec, Bartłomiej; Osella, Silvio; Trzaskowski, Bartosz; Jaramillo-Botero, Andrés; Pierpaoli, Mattia; Rycewicz, Michał; Dashkevich, Yanina; Łęga, Tomasz; Malinowska, Natalia; Cebula, Zofia; Bigus, Daniel; Firganek, Daniel; Bięga, Ewelina; Dziąbowska, Karolina; Brodowski, Mateusz; Kowalski, Marcin; Panasiuk, Mirosława; Gromadzka, Beata; Żołędowska, Sabina; Nidzworski, Dawid; Pyrć, Krzysztof; Goddard, William A.

doi:10.1016/j.bios.2022.114222

Cited by 36 publications

(26 citation statements)

References 67 publications

(56 reference statements)

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“…This immunosensor could successfully detect NP with the LOD of 50 plaque-forming units/mL (PFU/mL) and specificity of 100% in nasopharyngeal samples. In another report, Białobrzeska et al investigated the label-free SARS-CoV-2 NP immunosensing performance based on a series of substrates, including Au, glassy carbon (GC), and boron-doped diamond electrode (BDD) (Figure 6b) [90].…”

Section: Detection Of Nucleocapsid Proteinmentioning

confidence: 99%

Progress in Electrochemical Biosensing of SARS-CoV-2 Virus for COVID-19 Management

Rahman

2022

Chemosensors

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Rapid and early diagnosis of lethal coronavirus disease-19 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an important issue considering global human health, economy, education, and other activities. The advancement of understanding of the chemistry/biochemistry and the structure of the SARS-CoV-2 virus has led to the development of low-cost, efficient, and reliable methods for COVID-19 diagnosis over “gold standard” real-time reverse transcription-polymerase chain reaction (RT-PCR) due to its several limitations. This led to the development of electrochemical sensors/biosensors for rapid, fast, and low-cost detection of the SARS-CoV-2 virus from the patient’s biological fluids by detecting the components of the virus, including structural proteins (antigens), nucleic acid, and antibodies created after COVID-19 infection. This review comprehensively summarizes the state-of-the-art research progress of electrochemical biosensors for COVID-19 diagnosis. They include the detection of spike protein, nucleocapsid protein, whole virus, nucleic acid, and antibodies. The review also outlines the structure of the SARS-CoV-2 virus, different detection methods, and design strategies of electrochemical SARS-CoV-2 biosensors by highlighting the current challenges and future perspectives.

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Section: Detection Of Nucleocapsid Proteinmentioning

confidence: 99%

Progress in Electrochemical Biosensing of SARS-CoV-2 Virus for COVID-19 Management

Rahman

2022

Chemosensors

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“…Recently, electrochemical methods have received increased attention due to advantages such as fast response, low use of reagents, and high selectivity and sensitivity, making them suitable for custom-made sensors [ 12 ]. A fast search on the ScienceDirect platform by using “point-of-care detection” as a keyword has generated 365,181 results, including articles, reviews, and book chapters.…”

Section: Point-of-care Electrochemical Testingmentioning

confidence: 99%

“…The N protein is the main component of the ncovNP and is responsible for packaging and protecting coronavirus the genomic RNA, being divided into five distinct regions (Fig. 3B ) [ 12 ]. The S protein is cleaved into two separate polypeptides S1 and S2 within the host by a furin-like protease [ 113 ].…”

Section: Biomarkers Involved In Inflammatory Diseasesmentioning

confidence: 99%

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Point-of-care electrochemical testing of biomarkers involved in inflammatory and inflammatory-associated medical conditions

et al. 2022

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Recent years have shown that the diagnosis and monitoring of biomarkers involved in inflammatory-associated medical conditions such as cancer, neurological disorders, viral infections, or daily physical activities offer real benefits in increasing the quality of medical care and patient life quality. In this context, the use of integrated and portable platforms as point-of-care testing devices for biomedical analysis to enable early disease diagnosis and monitoring, which can be successfully used even at the patient’s bed, is an emergency nowadays. The development of low-cost, miniaturized, and portable, user-friendly devices that provide an answer in a timely manner, such as electrochemical sensors, is relevant for the elaboration of point-of-care testing devices. This review focuses on the recent progress in bioanalysis of both specific biomarkers and inflammatory-associated biomarkers present in several diseases like neoplasia, severe neurological disorders, viral infections, and usual physical activity and provides an overview of the state of the art over the most recent electrochemical (bio)sensors for the detection of inflammation-related biomarkers. Future perspectives of point-of-care testing to improve healthcare management are also discussed. Graphical abstract

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“…[42][43] This is due to the favourable sp 3 hybridisation of the sulphur atom in BMT, causing a 104° angle with the surface to the S-C bond, respectively, and allowing a densely packed monolayer. 44 Another common derivative of BT is aminothiophenol (ATP), which has an amine group, allowing attachment to a biorecognition element, as demonstrated by Billah et al 45 Vert recently, Białobrzeska et al 46 reported the development of an impedimetric biosensor for clinical diagnostics of SARS-CoV-2 based on selective nucleocapsid N protein detection. Researchers modified the gold surface with 4-ATP in order to obtain an amine functional surface.…”

Section: Thiols and Sulphidesmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy (EIS) Based Label-Free Immunosensors

Robinson

Juska

O’Riordan

2022

Preprint

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Electrochemical Impedance Spectroscopy (EIS) is a surface sensitive technique which examines the impedance of an electrochemical cell over a range of frequencies. By immobilising an antigen or antibody to the electrode surface, EIS shows potential for highly specific and sensitive immunosensor performance. Following an introduction into antibodies and EIS, this review presents immobilisation techniques for an immunosensor’s bioreceptor. It highlights electrode modifications such as self-assembled monolayers (SAMs), polymers and nanostructures for anchoring the bioreceptor to the surface. Stable immobilisation directly to these modifications is not always possible. Immobilisation techniques to these modified surfaces are explored including cross-linking and controlled orientation. EIS allows for label-free detection by monitoring the change in charge-transfer resistance or capacitance caused by changes in the electrode surface. Label-free immunosensors are the focus of this paper as they show potential for point-of-care (POC) devices.

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Performance of electrochemical immunoassays for clinical diagnostics of SARS-CoV-2 based on selective nucleocapsid N protein detection: Boron-doped diamond, gold and glassy carbon evaluation

Cited by 36 publications

References 67 publications

Progress in Electrochemical Biosensing of SARS-CoV-2 Virus for COVID-19 Management

Progress in Electrochemical Biosensing of SARS-CoV-2 Virus for COVID-19 Management

Point-of-care electrochemical testing of biomarkers involved in inflammatory and inflammatory-associated medical conditions

Electrochemical Impedance Spectroscopy (EIS) Based Label-Free Immunosensors

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