Performance of different methanogenic species for the microbial electrosynthesis of methane from carbon dioxide

Mayer, Florian; Enzmann, Franziska; López, Antonio Martínez; Holtmann, Dirk

doi:10.1016/j.biortech.2019.121706

Cited by 74 publications

(42 citation statements)

References 46 publications

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“…However, these experiments were carried out under conditional typically associated with electrochemical H 2 -generation 44 , and for a time frame of only 3 days, which is too short to test for the formation of electrical contacts for direct electromethanogenesis 11,45 . In fact, the authors observed only the growth of strict hydrogenotrophic methanogens on cathodes poised at −700 mV 43 . Strict hydrogenotrophic methanogens have low H 2 -thresholds (ca.…”

Section: Resultsmentioning

confidence: 97%

“…Recently, it was reported that M. mazei was not electroactive and incapable to retrieve electrons from a poised cathode at −700 mV (vs. SHE) 43 . However, these experiments were carried out under conditional typically associated with electrochemical H 2 -generation 44 , and for a time frame of only 3 days, which is too short to test for the formation of electrical contacts for direct electromethanogenesis 11,45 .…”

Section: Resultsmentioning

confidence: 99%

“…300 nM) like all other Methanosarcinales 46 . Unlike the study by Meyer et al 43 , our experiements were run at −400 mV vs. SHE, condition that does not allow for abiotic electrochemical H 2 accumulation even after several months 11 . In a recent study, we showed that a cathode at −400 mV could not be used as sole electron donor by H 2 -utilizing methanogens like Methanobacterium formicicum, but it was used by a strain capable of direct electron uptake (via DIET) -Methanosarcina barkeri strain MS 11 .…”

Section: Resultsmentioning

confidence: 99%

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Extracellular electron uptake in Methanosarcinales is independent of multiheme c-type cytochromes

Yee

Rotaru

2020

Sci Rep

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the co-occurrence of Geobacter and Methanosarcinales is often used as a proxy for the manifestation of direct interspecies electron transfer (Diet) in the environment. Here we tested eleven new coculture combinations between methanogens and electrogens. previously, only the most electrogenic Geobacter paired by Diet with Methanosarcinales methanogens, namely G. metallireducens and G. hydrogenophilus. Here we provide additional support, and show that five additional Methanosarcinales paired with G. metallireducens, while a strict hydrogenotroph could not. We also show that G. hydrogenophilus, which is incapable to grow with a strict hydrogenotrophic methanogen, could pair with a strict non-hydrogenotrophic Methanosarcinales. Likewise, an electrogen outside the Geobacter cluster (Rhodoferrax ferrireducens) paired with Methanosarcinales but not with strict hydrogenotrophic methanogens. the ability to interact with electrogens appears to be conserved among Methanosarcinales, the only methanogens with c-type cytochromes, including multihemes (MHc). nonetheless, MHc, which are often linked to extracellular electron transfer, were neither unique nor universal to Methanosarcinales and only two of seven Methanosarcinales tested had MHc. of these two, one strain had an MHc-deletion knockout available, which we hereby show is still capable to retrieve extracellular electrons from G. metallireducens or an electrode suggesting an MHcindependent strategy for extracellular electron uptake.Direct interspecies electron transfer (DIET) was discovered in an artificial co-culture of an ethanol-oxidizing Geobacter metallireducens with a fumarate-reducing Geobacter sulfurreducens where the possibility of hydrogen gas (H 2 ) or formate transfer was invalidated through genetic studies 1,2 . Gene deletions rendering H 2 and formate transfer impossible resulted in active co-cultures 1,2 , whereas deletion of genes for extracellular electron transfer proteins (EET) such as pili, and multiheme c-type cytochromes made the interspecies interaction impossible 1,3 . Remarkably, a deletion mutant lacking an outer membrane multiheme c-type cytochrome (OmcS) could be rescued by the addition of extracellular conductive particles, whereas a pili knock-out strain could not be rescued by conductive particles 4 . Additionally, previous studies have shown that G. metallireducens is also highly effective as an anode-respiring bacteria (ARB) generating some of the highest current densities of all Geobacter tested 5 . During DIET with G. sulfurreducens, G. metallireducens requires pili and certain multiheme c-type cytochromes 3 , which were also required during anode respiration 6,7 .G. metallireducens is a strict respiratory microorganism unable to ferment its substrates to produce H 2 for interspecies H 2 -transfer 8,9 . Consequently, G. metallireducens could not provide reducing equivalents for strict hydrogenotrophic methanogens (Methanospirilum hungatei and Methanobacterium formicicum) 10,11 . However, G. metallireducens did interact syntrophically wi...

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Extracellular electron uptake in Methanosarcinales is independent of multiheme c-type cytochromes

Yee

Rotaru

2020

Sci Rep

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“…is exporting electrons through its conductive pili [50,111,112]; however, the mechanism by which electrons are received by methanogen is still vaguely understood [12]. In BES-AD studies, various species could be enriched, such as Methanococcus maripaludis [119] and Methanobacterium congolense [120]. The type of enriched consortia might be related to the utilized electrode and the utilized catalyst [121].…”

Section: Microbial Community Change After Diet Stimulationmentioning

confidence: 99%

Enhanced Anaerobic Digestion by Stimulating DIET Reaction

Mostafa

Song

et al. 2020

Processes

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Since the observation of direct interspecies electron transfer (DIET) in anaerobic mixed cultures in 2010s, the topic “DIET-stimulation” has been the main route to enhance the performance of anaerobic digestion (AD) under harsh conditions, such as high organic loading rate (OLR) and the toxicants’ presence. In this review article, we tried to answer three main questions: (i) What are the merits and strategies for DIET stimulation? (ii) What are the consequences of stimulation? (iii) What is the mechanism of action behind the impact of this stimulation? Therefore, we introduced DIET history and recent relevant findings with a focus on the theoretical advantages. Then, we reviewed the most recent articles by categorizing how DIET reaction was stimulated by adding conductive material (CM) and/or applying external voltage (EV). The emphasis was made on the enhanced performance (yield and/or production rate), CM type, applied EV, and mechanism of action for each stimulation strategy. In addition, we explained DIET-caused changes in microbial community structure. Finally, future perspectives and practical limitations/chances were explored in detail. We expect this review article will provide a better understanding for DIET pathway in AD and encourage further research development in a right direction.

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“…Mainly, carbon based anode materials commonly used in bioelectrochemistry were chosen and compared. As an example process for MES, the bioelectrochemical production of methane by the electroactive methanogen Methanococcus maripaludis was chosen, which was already described in literature . Not only different anode materials, but also different anolytes are investigated and compared.…”

Section: Introductionmentioning

confidence: 99%

Insights in the anode chamber influences on cathodic bioelectromethanogenesis – systematic comparison of anode materials and anolytes

Enzmann

Stöckl

Gronemeier

et al. 2019

Engineering in Life Sciences

Self Cite

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Cathode and catholyte are usually optimized to improve microbial electrosynthesis process, whereas the anodic counter reaction was not systematically investigated and optimized for these applications yet. Nevertheless, the anolyte and especially the anode material can limit the cathodic bioelectrochemical process. This paper compares for the first time the performance of different anode materials as counter electrodes for a cathodic bioelectrochemical process, the bioelectromethanogenesis. It was observed that depending on the anode material the cathodic methane production varies from 0.96 µmol/d with a carbon fabric anode to 25.44 µmol/d with a carbon felt anode of the same geometrical surface area. The used anolyte also affected the methane production rate at the cathode. Especially, the pH of the anolyte showed an impact on the system; an anolyte with pH 5 produced up to 2.0 times more methane compared to one with pH 8.5. The proton availability is discussed as one reason for this effect. Although some of the measured effects cannot be explained completely so far this study advises researchers to strongly consider the anode impact during process development and optimization of a cathodic bioelectrochemical synthesis process.

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Performance of different methanogenic species for the microbial electrosynthesis of methane from carbon dioxide

Cited by 74 publications

References 46 publications

Extracellular electron uptake in Methanosarcinales is independent of multiheme c-type cytochromes

Extracellular electron uptake in Methanosarcinales is independent of multiheme c-type cytochromes

Enhanced Anaerobic Digestion by Stimulating DIET Reaction

Insights in the anode chamber influences on cathodic bioelectromethanogenesis – systematic comparison of anode materials and anolytes

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