Performance of current microbial tests for screening antibiotics in sheep and goat milk

Beltrán, M.C.; Berruga, M.I.; Molina, A.; Althaus, R. L.; Molina, M.P.

doi:10.1016/j.idairyj.2014.09.007

Cited by 49 publications

(42 citation statements)

References 9 publications

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“…Thus, the detection rates for this group of antimicrobials are higher than those obtained for families other than beta-lactams, especially for drugs belonging to the tetracycline and quinolone groups that were not detected at safety levels by any of the microbial tests considered. These results were in agreement with those obtained by other authors in milk from these species several years ago (Althaus et al, 2003;Molina et al, 2003;Montero et al, 2005;Sierra et al, 2009a,b), although it is noteworthy that some drugs such as neomycin, tylosin and sulfadimethoxine can be more easily detected by the new versions of microbial test commercially available (Beltrán et al, 2015).…”

Section: Discussionsupporting

confidence: 92%

Analytical strategy for the detection of antibiotic residues in sheep and goat’s milk

Beltrán

Althaus

Molina

et al. 2015

Span. j. agric. res.

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The use of antibiotics to treat mastitis and other infectious diseases in dairy sheep and goats is a widespread practice nowadays that can, when not properly applied, result in the contamination of the milk supply. Spanish legislation establishes the control of the presence of antibiotic residues in sheep and goat’s milk using screening methods that detect, at least, beta-lactam drugs. Microbial inhibitor tests using Geobacillus stearothermophilus var. calidolactis and specific receptor-binding assays are most widely employed for this purpose. The detection rates of screening tests routinely used in Spain have been calculated considering the frequency of use of veterinary drugs commonly applied in ovine and caprine livestock to treat and prevent mastitis as well as the test sensitivity toward these substances at safety levels. In general, the use of a single test allows detecting 62.8-82.4% of the antibiotics employed. For sheep milk, the total detection range achieved with microbial tests was significantly higher than that reached with rapid receptor tests. However, no significant differences between the two types of methods were found when goat's milk was analysed. In both types of milk, the simultaneous use of two screening tests with a different analytical basis increases the total detection range significantly, reaching values ≥ 90% in some cases (81.5-90.1% for sheep and 84.7-92.6% for goats). However, the periodical use of screening tests able to detect quinolones, macrolides or aminoglycosides would be recommended to carry out more efficient screening and ensure the safety of milk and dairy products from sheep and goats.

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Section: Discussionsupporting

confidence: 92%

Analytical strategy for the detection of antibiotic residues in sheep and goat’s milk

Beltrán

Althaus

Molina

et al. 2015

Span. j. agric. res.

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“…Antibiotic‐free goat's milk employed for yoghurt production showed a good hygienic quality and similar physicochemical characteristics to those reported by other authors for the Murciano‐Granadina breed of goats (Beltrán et al . ). The gross chemical composition (g/100 g) was as follows: total solids 15.32, fat 5.94, protein 4.03.…”

Section: Resultsmentioning

confidence: 97%

“…; Beltrán et al . ). Thus, the presence of such substances in raw milk may remain undetected in the screening phase and finally reach the dairy industry where, in spite of the treatments applied in the production and storage process, elevated amounts of this antibiotic may be found in yoghurts.…”

Section: Resultsmentioning

confidence: 97%

Influence of enrofloxacin on the coagulation time and the quality parameters of goat's milk yoghurt

Beltrán

Morari‐Pirlog

Quintanilla

et al. 2017

Int J of Dairy Tech

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Three batches of yoghurts were made from goat's milk with different enrofloxacin concentrations (0, 50, 100 and 150 lg/kg). Quality parameters were analysed at 1, 7, 14 and 28 days at 5°C. Drug residues were also quantified by HPLC. Coagulation time and most yoghurt properties remained unaffected by the presence of enrofloxacin in goat's milk. However, quality parameters were affected by the storage period. 74.9-99.2% of enrofloxacin initially added to goat's milk remained in the yoghurt throughout its entire shelf life, potentially posing a risk to consumer health. Therefore, an enrofloxacin maximum residue limit in yoghurt should be established.

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“…Moreover, sometimes dairy industries illegally addlactamase in milk to degrade -lactams antibiotics [14,16,17]. Such illegal practice may reduce -lactams concentration, but it exposes consumers to risks to the health since the safety of -lactamase has not been fully evaluated [17].…”

Section: Journal Of Sensorsmentioning

confidence: 99%

Feasibility Study on a Measurement Method and a Portable Measuring System to Estimate the Concentration of Cloxacillin andβ-Lactamase in Milk

et al. 2017

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Antibiotics are nowadays used and abused worldwide in common veterinary practice to treat diseases, prevent infections, and promote animal's growth. Drug resistance occurrence is a relevant phenomenon that can inactivate the antibiotic. Moreover, antibiotics used for animals are also found in milk and it poses serious health risks to humans. Another negative effect is related to milk processors since antibiotics cause detrimental effects on cheese and yogurt starter bacteria. Given its consumption as both beverage and derivatives, milk is one of the most regulated products in food industry. Nowadays several commercial tests are available to investigate antibiotics in milk, but they generally provide a qualitative result, require bulky procedure, and are time consuming. In this paper, we investigate the use of a chromogenic cephalosporin to quantify the concentration of cloxacillin-a -lactam difficult to be detected by using "cowside" screenings which is the drug of choice in the method of mastitis control. The proposed measurement method and prototype have been demonstrated to be able to detect cloxacillin in milk at concentrations lower than the MRL set by the European Commission. Moreover, they are also able to detect the illegal practice of using -lactamase to degrade -lactams in milk.

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Performance of current microbial tests for screening antibiotics in sheep and goat milk

Cited by 49 publications

References 9 publications

Analytical strategy for the detection of antibiotic residues in sheep and goat’s milk

Analytical strategy for the detection of antibiotic residues in sheep and goat’s milk

Influence of enrofloxacin on the coagulation time and the quality parameters of goat's milk yoghurt

Feasibility Study on a Measurement Method and a Portable Measuring System to Estimate the Concentration of Cloxacillin andβ-Lactamase in Milk

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