Performance of conventional histopathology and GeneXpert MTB/RIF in the diagnosis of spinal tuberculosis from bone specimens: A prospective clinical study

Yu, Yali; Kong, Yiyi; Ye, Jing; Wang, Aiguo

doi:10.1016/j.clinbiochem.2020.08.010

Cited by 14 publications

(6 citation statements)

References 24 publications

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“…Although CRS can underestimate the true sensitivity (Yu et al . 2020), we achieved a moderately good sensitivity of ~73% and specificity of ~91–94% by I‐PCR based on Ag85 detection against CRS, which may be further improved so as to meet the WHO (2014) criteria (sensitivity of ≥80% and specificity of ≥98% for EPTB specimens) based on high‐priority target product profile. Although quantitative RT‐I‐PCR employed in this study could monitor the progression of disease, no substantial improvement in sensitivity was observed by RT‐I‐PCR when compared with I‐PCR.…”

Section: Resultsmentioning

confidence: 79%

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Diagnosis of osteoarticular tuberculosis by immuno‐PCR assay based on mycobacterial antigen 85 complex detection

Khan

Singh

Sharma

et al. 2021

Letters Applied Microbiology

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Diagnosis of osteoarticular tuberculosis (OATB) exhibits serious challenges owing to paucibacillary nature of specimens and localization of disease at sites that are difficult to access. We recently developed indirect immuno‐PCR (I‐PCR) and real‐time I‐PCR (RT‐I‐PCR) assays for the detection of mycobacterial antigen 85 complex (Ag85) in OATB patients. Detection limits for the purified Ag85 protein were found to be 1 and 41 fg ml−1 by I‐PCR and RT‐I‐PCR, respectively, which were at least 105‐fold lower than respective ELISA. While spiking synovial fluids of non‐TB control subjects with the purified Ag85 protein, LODs of 100 and 120 fg ml−1 were obtained by I‐PCR and RT‐I‐PCR, respectively, thus demonstrating the sample matrix effect. Sensitivities of 87·5 and 70·5% were observed in bodily fluids of confirmed (n = 8) and clinically suspected (n = 51) OATB cases, respectively, by I‐PCR, with a specificity of 93·9% (n = 33). Markedly, the sensitivities obtained by I‐PCR/RT‐I‐PCR were significantly higher (P < 0·05–0·01) than ELISA and GeneXpert assay (n = 30). However, no substantial difference in sensitivity was observed between the I‐PCR and RT‐I‐PCR assays. After further improving the accuracy of I‐PCR, this test may lead to development of an attractive diagnostic kit.

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Section: Resultsmentioning

confidence: 79%

“…2018; Yu et al . 2020). The present findings and the previous reports recommend that Xpert could be used as an excellent rule‐in test for diagnosing OATB cases but not as a promising rule‐out test.…”

Section: Resultsmentioning

confidence: 99%

Diagnosis of osteoarticular tuberculosis by immuno‐PCR assay based on mycobacterial antigen 85 complex detection

Khan

Singh

Sharma

et al. 2021

Letters Applied Microbiology

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“…The GeneXpert system, capable of rapidly identifying MTB with rifampin (RIF) resistance within 2 h, has been widely investigated using a wide variety of samples, including paraffin-embedded tissues ( 11 ), blood samples ( 19 ), sterile body fluids ( 20 ), bones ( 21 ), and extrapulmonary samples ( 13 , 22 ), thus proving that GeneXpert is a valuable tuberculosis diagnostic tool. In comparison, the culture of MTB requires a longer cycle (usually 14 to 28 days).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Performances of GeneXpert MTB/RIF, Bactec MGIT 960, and Bactec Myco/F Systems in Detecting Mycobacterium tuberculosis in Biopsy Tissues: a Retrospective Study

Zhao

Zhang

et al. 2023

Microbiol Spectr

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“…A high index of clinical suspicion is the mainstay to the diagnosis of spinal TB. On biopsy, in addition to culture, molecular testing can be performed with Xpert/RIF sensitivity of 82-95.6% and specificity of 96.2-100% to detect spinal TB [45,46]. Material for detection of M. tuberculosis can be acquired via bone biopsy, with limited availability in resource-limited settings.…”

Section: Diagnosismentioning

confidence: 99%

Central nervous system tuberculosis

Dian

Ganiem

Laarhoven

2021

Current Opinion in Neurology

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Purpose of review Central nervous system (CNS) tuberculosis is the most devastating form of tuberculosis (TB), with mortality and or neurological sequelae in over half of individuals. We reviewed original research and systematic reviews published since 1 January 2019 for new developments in CNS TB pathophysiology, diagnosis, management and prognosis. Recent findings Insight in the pathophysiology is increasing steadily since the landmark studies in 1933, focussing on granuloma type classification, the relevance of the M. tuberculosis bacterial burden and the wide range of immunological responses. Although Xpert/RIF has been recommended by the WHO for extrapulmonary TB diagnosis, culture is still needed to increase the sensitivity of TB meningitis diagnosis. Sequential MRIs can improve understanding of neurological deficits at baseline and during treatment. Pharmacokinetic/pharmacodynamic modelling suggests that higher doses of rifampicin and isoniazid in TB meningitis could improve survival. Summary Recent studies in the field of CNS-TB have largely focussed on TB meningitis. The outcome may improve by optimizing treatment dosing. This needs to be confirmed in clinical trials. Due to the important role of inflammation, these trials should be used as the platform to study the inflammatory and metabolomic responses. This could improve understanding of the biology of this disease and improve patient outlook by enabling individualised host-directed therapy.

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Performance of conventional histopathology and GeneXpert MTB/RIF in the diagnosis of spinal tuberculosis from bone specimens: A prospective clinical study

Cited by 14 publications

References 24 publications

Diagnosis of osteoarticular tuberculosis by immuno‐PCR assay based on mycobacterial antigen 85 complex detection

Diagnosis of osteoarticular tuberculosis by immuno‐PCR assay based on mycobacterial antigen 85 complex detection

Comparison of Performances of GeneXpert MTB/RIF, Bactec MGIT 960, and Bactec Myco/F Systems in Detecting Mycobacterium tuberculosis in Biopsy Tissues: a Retrospective Study

Central nervous system tuberculosis

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