Performance of columns packed with the new shell particles, Kinetex-C18

Gritti, Fabrice; Leonardis, Irene; Shock, David; Stevenson, Paul; Shalliker, Andrew; Guiochon, Georges

doi:10.1016/j.chroma.2009.12.079

Cited by 202 publications

(118 citation statements)

References 39 publications

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“…Likewise, 4Ј-hydroxydiclofenac and 5-hydroxydiclofenac produced identical daughter ions at m/z 266, 230, 194, and 166. For these sample sets, we found that the new shell particle technology provided the highest separation efficiency while maintaining a low chromatography system back pressure (Gritti et al, 2010). Adequate separation of five of the six tested drug-metabolites combinations was achieved, using a single, long default gradient on a Kinetex C18 column.…”

Section: Direct and Quantitative Evaluation Of Mist Using Lc-ms/msmentioning

confidence: 95%

A Simple Liquid Chromatography-Tandem Mass Spectrometry Method to Determine Relative Plasma Exposures of Drug Metabolites across Species for Metabolite Safety Assessments

Gao¹,

Deng²,

Obach³

2010

Drug Metab Dispos

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ABSTRACT:Recent regulatory guidance suggests that metabolites identified in human plasma should be present at equal or greater levels in one of the animal species used in safety assessments. In this report, a high-performance liquid chromatography-tandem mass spectrometry method is described whereby quantitative comparisons of exposures to metabolites between species can be obtained in the absence of authentic standards of the metabolites, calibration curves, and other attributes of standard bioanalytical methods. This novel method was tested using six drug-metabolite combinations. Plasma samples from animals are mixed with control plasma from humans and vice versa to remove possible differential effects of matrices. Through multiple ion monitoring-triggered enhanced product ion (EPI) scans, all metabolites were qualitatively confirmed, and daughter ions were selected for the most sensitive mass transitions to trigger EPI scans.Direct comparisons of metabolites in animal versus human plasma were achieved by calculating the peak area ratios of the metabolites versus an internal standard. Linearity of instrument responses was established by serial dilution. A statistical analysis demonstrated that experimentally measured ratios of the parent and metabolites in rat versus human correlated well with the nominal ratios of concentrations using linear regression with an average slope of 0.99 ؎ 0.08 (r ‫؍‬ 0.994 ؎ 0.005). This analysis showed that if the experimentally determined ratio of mass spectrometer responses is >2.0, then the actual exposure ratio is unity or greater (p < 0.01). This method offers timeand resource-sparing advantages to ascertaining metabolite exposure comparisons between humans and laboratory animal species. A strategy for application of this approach within standard drug development processes is described.

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Section: Direct and Quantitative Evaluation Of Mist Using Lc-ms/msmentioning

confidence: 95%

A Simple Liquid Chromatography-Tandem Mass Spectrometry Method to Determine Relative Plasma Exposures of Drug Metabolites across Species for Metabolite Safety Assessments

Gao¹,

Deng²,

Obach³

2010

Drug Metab Dispos

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“…Consequently, extracolumn effects attributed to instrumentation that reduce the measured separation efficiency are more prominent than with standard HPLC columns [1][2][3][4][5][6][7][8][9][10][11]. Similar to previous eras when column technology began to exceed the limitations of commercial equipment [12,13], extra-column band broadening (ECBB) is now seeing increasing attention as an important factor that needs to be accounted for and reduced as much as possible [14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 94%

Measurement and Modeling of Extra-Column Effects Due to Injection and Connections in Capillary Liquid Chromatography

et al. 2015

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Abstract:As column volumes continue to decrease, extra-column band broadening has become an increasingly important consideration when determining column performance. Combined contributions due to the injector and connecting tubing in a capillary LC system were measured and found to be larger than expected by Taylor-Aris theory. Variance from sigma-type and tau-type broadening was isolated from eluted peaks using the Foley-Dorsey Exponentially Modified Gaussian peak fitting model and confirmed with computational fluid dynamics. It was found that the tau-type contributions were the main cause for the excessive broadening because of poorly-swept volumes at the connection between the injector and tubing. To reduce tau-type contributions (and peak tailing), a timed pinch mode could be used for analyte injection.

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“…Superficially porous (core/ shell) sub-3 µm silica particles have also been prepared recently. 2,3,7,10,[19][20][21][22][23][24][25][26][27][28][29][30] Nonporous Silica Particles. Nonporous silica particles have been used for quick separation of peptides and proteins.…”

Section: Sub 3 µM Silica Particlesmentioning

confidence: 99%

Porous Silica Particles As Chromatographic Separation Media: A Review

Cheong¹

2014

Bulletin of the Korean Chemical Society

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Porous silica particles are the most prevailing raw material for stationary phases of liquid chromatography. During a long period of time, various methodologies for production of porous silica particles have been proposed, such as crashing and sieving of xerogel, traditional dry or wet process preparation of conventional spherical particles, preparation of hierarchical mesoporous particles by template-mediated pore formation, repeated formation of a thin layer of porous silica upon nonporous silica core (core-shell particles), and formation of specific silica monolith followed by grinding and calcination. Recent developments and applications of useful porous silica particles will be covered in this review. Discussion on sub-3 µm silica particles including nonporous silica particles, carbon or metal oxide clad silica particles, and molecularly imprinted silica particles, will also be included. Next, the individual preparation methods and their feasibilities will be collectively and critically compared and evaluated, being followed by conclusive remarks and future perspectives.

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Performance of columns packed with the new shell particles, Kinetex-C18

Cited by 202 publications

References 39 publications

A Simple Liquid Chromatography-Tandem Mass Spectrometry Method to Determine Relative Plasma Exposures of Drug Metabolites across Species for Metabolite Safety Assessments

A Simple Liquid Chromatography-Tandem Mass Spectrometry Method to Determine Relative Plasma Exposures of Drug Metabolites across Species for Metabolite Safety Assessments

Measurement and Modeling of Extra-Column Effects Due to Injection and Connections in Capillary Liquid Chromatography

Porous Silica Particles As Chromatographic Separation Media: A Review

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