Performance of collagen sponge as a 3-D scaffold for tooth-tissue engineering

Sumita, Yoshinori; Honda, Masaki; Ohara, Takayuki; Tsuchiya, Shunji; Sagara, Hiroshi; Kagami, Hideaki; Ueda, Minoru

doi:10.1016/j.biomaterials.2006.01.055

Cited by 177 publications

(127 citation statements)

References 19 publications

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“…Different matrix constituents have been used for tooth engineering (Table 1). However, collagen, under different forms, was by far the most frequently used (Honda et al, 2007;Nakao et al, 2007;Sumita et al, 2006). Collagen gel has been successfully used for dental cells encapsulation, as obviously neither interfering with the epithelial histogenesis nor with the patterning of cell differentiation (Arany et al, 2009;Nakao et al, 2007).…”

Section: Extracellular Matrix Constituentsmentioning

confidence: 99%

Tooth Organ Engineering: Biological Constraints Specifying Experimental Approaches

Kuchler‐Bopp¹,

Keller²,

Poliard³

et al. 2011

Tissue Engineering for Tissue and Organ Regeneration

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Section: Extracellular Matrix Constituentsmentioning

confidence: 99%

Tooth Organ Engineering: Biological Constraints Specifying Experimental Approaches

Kuchler‐Bopp¹,

Keller²,

Poliard³

et al. 2011

Tissue Engineering for Tissue and Organ Regeneration

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“…Promising research has shown that postnatal DE and dental mesenchymal (DM) cells, seeded together onto a variety of synthetic and natural scaffolds, can form very small tooth 689082J DRXXX10.1177/0022034516689082Journal of Dental ResearchDecellularized Tooth Bud Scaffolds for Tooth Regeneration research-article2017 crowns (Young et al 2002;Sumita et al 2006;Zhang et al 2009). In contrast, the novelty of the approach described here is the use of decellularized TB (dTB) scaffolds, created from natural porcine TBs, to successfully regenerate teeth of specified size and shape.…”

Section: Introductionmentioning

confidence: 99%

Decellularized Tooth Bud Scaffolds for Tooth Regeneration

et al. 2017

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Whole tooth regeneration approaches currently are limited by our inability to bioengineer full-sized, living replacement teeth. Recently, decellularized organ scaffolds have shown promise for applications in regenerative medicine by providing a natural extracellular matrix environment that promotes cell attachment and tissue-specific differentiation leading to full-sized organ regeneration. We hypothesize that decellularized tooth buds (dTBs) created from unerupted porcine tooth buds (TBs) can be used to guide reseeded dental cell differentiation to form whole bioengineered teeth, thereby providing a potential off-the-shelf scaffold for whole tooth regeneration. Porcine TBs were harvested from discarded 6-mo-old pig jaws, and decellularized by successive sodium dodecyl sulfate/Triton-X cycles. Four types of replicate implants were used in this study: 1) acellular dTBs; 2) recellularized dTBs seeded with porcine dental epithelial cells, human dental pulp cells, and human umbilical vein endothelial cells (recell-dTBs); 3) dTBs seeded with bone morphogenetic protein (BMP)-2 (dTB-BMPs); and 4) freshly isolated nondecellularized natural TBs (nTBs). Replicate samples were implanted into the mandibles of host Yucatan mini-pigs and grown for 3 or 6 mo. Harvested mandibles with implanted TB constructs were fixed in formalin, decalcified, embedded in paraffin, sectioned, and analyzed via histological methods. Micro-computed tomography (CT) analysis was performed on harvested 6-mo samples prior to decalcification. All harvested constructs exhibited a high degree of cellularity. Significant production of organized dentin and enamel-like tissues was observed in dTB-recell and nTB implants, but not in dTB or dTB-BMP implants. Micro-CT analyses of 6-mo implants showed the formation of organized, bioengineered teeth of comparable size to natural teeth. To our knowledge, these results are the first to describe the potential use of dTBs for functional whole tooth regeneration.

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“…These approaches are categorized according to whether the cell source derives from post-natal-or embryonic-tooth bud cells (13,16,17,21,40,41,63), and results from these two sources differ considerably. When porcine post-natal tooth buds at late-bell stages are used, tissues are regenerated with structures containing dentin and enamel (14,15,52,63). However, most produced tissues exhibit a disorganized heterogeneous morphology (14,15,52,63).…”

mentioning

confidence: 99%

“…When porcine post-natal tooth buds at late-bell stages are used, tissues are regenerated with structures containing dentin and enamel (14,15,52,63). However, most produced tissues exhibit a disorganized heterogeneous morphology (14,15,52,63). On the other hand, our previous study showed that mouse molar tooth bud cells at the cap stage of embryonic day 14 (ED14) are able to form teeth with regular crown shapes (21).…”

mentioning

confidence: 99%

<b>Induction of neural crest cells from human dental pulp-derived induced pluripotent stem </b><b>cells </b>

et al. 2017

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We previously generated induced pluripotent stem (iPS) cells from human dental pulp cells of deciduous teeth. Neural crest cells (NCCs) play a vital role in the development of the oral and maxillofacial region. Therefore, NCCs represent a cell source for bone, cartilage, and tooth-related tissue engineering. In this study, we examined whether iPS cells are capable of differentiating into NCCs through modification of the human embryonic stem cell protocol. First, iPS cells were dissociated into single cells and then reaggregated in low-cell-adhesion plates with neural induction medium for 8 days in suspension culture to form neurospheres. The neurospheres were transferred to fibronectin-coated dishes and formed rosette structures. The migrated cells from the rosettes abundantly expressed NCC markers, as evidenced by real-time polymerase chain reaction, immunofluorescence, and flow cytometric analysis. Furthermore, the migrated cells exhibited the ability to differentiate into neural crest lineage cells in vitro. They also exhibited tissue-forming potential in vivo, differentiating into bone and cartilage. Collectively, the migrated cells had similar characteristics to those of NCCs. These results suggest that human dental pulp cell-derived iPS cells are capable of differentiating into NCCs. Therefore, iPS cell-derived NCCs represent cell sources for bone and cartilage tissue engineering.

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Performance of collagen sponge as a 3-D scaffold for tooth-tissue engineering

Cited by 177 publications

References 19 publications

Tooth Organ Engineering: Biological Constraints Specifying Experimental Approaches

Tooth Organ Engineering: Biological Constraints Specifying Experimental Approaches

Decellularized Tooth Bud Scaffolds for Tooth Regeneration

<b>Induction of neural crest cells from human dental pulp-derived induced pluripotent stem </b><b>cells </b>

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