Performance of CellaVision DM96 in leukocyte classification

Abstract

Background:Leukocyte differentials are an important component of clinical care. Morphologic assessment of peripheral blood smears (PBS) may be required to accurately classify leukocytes. However, manual microscopy is labor intensive. The CellaVision DM96 is an automated system that acquires digital images of leukocytes on PBS, pre-classifies the cell type, and displays them on screen for a Technologist or Pathologist to approve or reclassify. Our study compares the results of the DM96 with manual microscopy.Methods:Three hundred and fifty-nine PBS were selected and assessed by manual microscopy with a 200 leukocyte cell count. They were then reassessed using the CellaVision DM96 with a 115 leukocyte cell count including reclassification when necessary. Correlation between the manual microscopy results and the CellaVision DM96 results was calculated for each cell type.Results:The correlation coefficients (r2) range from a high of 0.99 for blasts to a low of 0.72 for metamyelocytes.Conclusions:The correlation between the CellaVision DM96 and manual microscopy was as good or better than the previously published data. The accuracy of leukocyte classification depended on the cell type, and in general, there was lower correlation for rare cell types. However, the correlation is similar to previous studies on the correlation of manual microscopy with an established reference result. Therefore, the CellaVision DM96 is appropriate for clinical implementation.

Do We Know Why We Make Errors in Morphological Diagnosis? An Analysis of Approach and Decision-Making in Haematological Morphology

“…However, biases related to heuristics are also evident. As microscope-side teaching of skills becomes less widespread, and computer-driven decision-support methods are introduced ( Ceelie et al, 2007; Lee et al, 2013; Crowley et al, 2013 ) it is important that we actively seek to identify, understand, and address how we arrive at rapid accurate conclusions or errors ( Hamilton et al, 2009 ).…”

Detection of Platelet Clumps on Peripheral Blood Smears by CellaVision DM96 System and Microscopic Review

“…overall reliability of each of these functions of the DM96 has been assessed by a number of investigators, [1][2][3][4][5][6][7][8] who found the functions to be acceptable for clinical use. However, to our knowledge, no published studies have assessed the reliability of this system in specifically detecting the presence of PLT clumps on peripheral blood smears.…”

Clinical Microbiology Informatics

“…The capture, transmission, and remote or automated analysis of photomicrographs is becoming a reality in pathology (67)(68)(69)(70). Remote analysis of microbiology (telemicrobiology) has been implemented only sparsely, but there is growing interest in the transmission of microbiology images for remote analysis and consultation (71).…”

“…Automated or semiautomated digital image analysis has been implemented successfully in cytology, cytogenetics, and hematology laboratories, in which repetitive, high-volume image analysis occurs (69,70). However, automated image interpretation in the clinical microbiology laboratory has not been widely implemented.…”