Performance of an infrared microspectrometer at the NSLS

Carr, G.L.; Reffner, John A.; Williams, Gwyn

doi:10.1063/1.1145951

Cited by 124 publications

(85 citation statements)

References 5 publications

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“…SR-FTIR spectromicroscopy imaging and data analysis SR-FTIR spectromicroscopy is a non-invasive and label-free chemical imaging technology that provides molecular information at micrometer spatial resolution (Carr et al, 1995;Dumas et al, 2009). SR-FTIR takes advantage of three technologies: (i) the well-known sensitivity of infrared spectroscopy to the bond vibration frequencies in a molecule for determining molecular functional groups, (ii) the convenience of a light microscope to locate areas for molecular and composition analysis, and (iii) the 100-to 1000-fold increase in signal-to-noise provided by a bright SR-based infrared light source.…”

Section: Molecular Analysis Controlsmentioning

confidence: 99%

Tackling the minority: sulfate-reducing bacteria in an archaea-dominated subsurface biofilm

et al. 2012

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Archaea are usually minor components of a microbial community and dominated by a large and diverse bacterial population. In contrast, the SM1 Euryarchaeon dominates a sulfidic aquifer by forming subsurface biofilms that contain a very minor bacterial fraction (5%). These unique biofilms are delivered in high biomass to the spring outflow that provides an outstanding window to the subsurface. Despite previous attempts to understand its natural role, the metabolic capacities of the SM1 Euryarchaeon remain mysterious to date. In this study, we focused on the minor bacterial fraction in order to obtain insights into the ecological function of the biofilm. We link phylogenetic diversity information with the spatial distribution of chemical and metabolic compounds by combining three different state-of-the-art methods: PhyloChip G3 DNA microarray technology, fluorescence in situ hybridization (FISH) and synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectromicroscopy. The results of PhyloChip and FISH technologies provide evidence for selective enrichment of sulfate-reducing bacteria, which was confirmed by the detection of bacterial dissimilatory sulfite reductase subunit B (dsrB) genes via quantitative PCR and sequence-based analyses. We further established a differentiation of archaeal and bacterial cells by SR-FTIR based on typical lipid and carbohydrate signatures, which demonstrated a co-localization of organic sulfate, carbonated mineral and bacterial signatures in the biofilm. All these results strongly indicate an involvement of the SM1 euryarchaeal biofilm in the global cycles of sulfur and carbon and support the hypothesis that sulfidic springs are important habitats for Earth's energy cycles. Moreover, these investigations of a bacterial minority in an Archaea-dominated environment are a remarkable example of the great power of combining highly sensitive microarrays with label-free infrared imaging.

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Section: Molecular Analysis Controlsmentioning

confidence: 99%

Tackling the minority: sulfate-reducing bacteria in an archaea-dominated subsurface biofilm

et al. 2012

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“…up to the micrometre scale. It thus provides unique probes for advanced molecular research on biosamples with good SNR at apertures of a few micrometres (Carr et al, 1995). As addressed above, SR is a non-thermal source and its spectral distribution at low energy (e.g.…”

Section: Principles Of Synchrotron-based Ftir Microscopymentioning

confidence: 99%

Facing the challenge of biosample imaging by FTIR with a synchrotron radiation source

Petibois

Piccinini

Guidi

et al. 2009

J Synchrotron Radiat

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Fourier-transform infrared (FTIR) synchrotron radiation (SR) microspectroscopy is a powerful molecular probe of biological samples at cellular resolution (<10 mm). As the brilliance of SR is 100-1000 times higher than that of a conventional Globar source, FTIR microscopes are now available in almost all advanced SR facilities around the world. However, in spite of this superior performance, the expected advances in IR SR microscopy have not yet been realised, particularly with regard to bio-analytical studies of single cells and soft tissues. In recent decades solid-state array detectors have revolutionized the fields of molecular spectroscopy and chemical imaging, and now new IR focal plane array detectors implemented at ultra-bright SR facilities will extend the performance and overcome the existing limitations, possibly allowing IR SR instrumentation to achieve the highest sensitivity and resolution of molecular imaging. The impact of IR imaging on large tissue area and the complexity of the analysis are discussed. In view of the high brilliance of SR sources, a comparison of published microscope images is given. Finally, it is briefly outlined how an optimized combination of IR instrumentation and SR optical systems could reach the expected advantages of a SR-based FTIR imaging system.

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“…Consequently, most designers choose to maximize the throughput of the system rather than achieve the best possible spatial resolution at the diffraction limit. Synchrotron sources, which are 100-1000 times brighter than a typical blackbody source [1][2][3][4][5][6][7][8] , have made diffraction-limited spatial resolutions possible. Here, we discuss a simple modification to commercial infrared microscopes that allows the user to take full advantage of the spatial resolution achievable by synchrotron infrared radiation.…”

Section: Introductionmentioning

confidence: 99%

Improved Spatial Resolution For Reflection Mode Infrared Spectromicroscopy

Bechtel¹,

Martin²,

May³

et al. 2010

AIP Conference Proceedings

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Abstract. Standard commercial infrared microscopes operating in reflection mode use a mirror to direct the reflected light from the sample to the detector. This mirror blocks about half of the incident light, however, and thus degrades the spatial resolution by reducing the numerical aperture of the objective. Here, we replace the mirror with a 50% beamsplitter to allow full illumination of the objective and retain a way to direct the reflected light to the detector. The improved spatial resolution is demonstrated using a microscope coupled to a synchrotron source.

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Performance of an infrared microspectrometer at the NSLS

Cited by 124 publications

References 5 publications

Tackling the minority: sulfate-reducing bacteria in an archaea-dominated subsurface biofilm

Tackling the minority: sulfate-reducing bacteria in an archaea-dominated subsurface biofilm

Facing the challenge of biosample imaging by FTIR with a synchrotron radiation source

Improved Spatial Resolution For Reflection Mode Infrared Spectromicroscopy

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