Performance of a physically adsorbed high-molecular-mass polyethyleneimine layer as coating for the separation of basic proteins and peptides by capillary electrophoresis

Abstract

Performance of a physically adsorbed high-molecular-mass polyethyleneimine layer as coating for the separation of basic proteins and peptides by capillary electrophoresis Erim, F.B.; Cifuentes, A.; Poppe, H.; Kraak, J.C. General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. AbstractA simple method for the preparation of a polyethyleneimine (PEI) coating on the inner surface of fused-silica capillaries for capillary electrophoresis (CE) is reported. The PEI layer can be coated on the silica surface by just flushing the capillary with a solution containing high-molecular-mass PEI. The physically adsorbed layer appears to be very stable and can be used in a pH range of 3-11. In comparison to described methods to fabricate an immobilized PEI layer, the proposed method does not require an immobilization step, is simple and the preparation time of the coating is less than two hours. Good reproducibilities of migration times of basic proteins and peptides were obtained on the same PEI-coated capillary as well as on different PEI-coated capillaries. For basic proteins efficiencies ranging from 300 000-500 000 plates per meter were normally found.

Catalytic Materials Based on Surface Coating with Poly(ethyleneimine)‐Stabilized Gold Nanoparticles

“…In addition, commercial silica‐PEI beads have been previously used to synthesize supported AuNPs, and the interaction between PAMAM and SiO 2 has been already exploited to prepare silica‐supported gold nanocomposites . Considering the fact that PEI efficiently coats the surface of metal oxides such as TiO 2 , ZrO 2 , and SnO 2 to yield stable amino‐functionalized particles that withstand pH 10, we decided to evaluate the suitability of MW‐PEI@AuNPs to functionalize silica . Simple addition of commercial silica to MW‐PEI@AuNPs turned the solution transparent and the silica reddish almost instantly (Figure S6).…”

“…It has been demonstrated that PEI acts as an efficient reductant of HAuCl 4 at both 60–80 °C and room temperature, with the size of the resulting NPs dependent on the bPEI:HAuCl 4 ratio . Additionally, PEI, as a polymeric amine with a high density of charge, has been used to improve the adhesion of different compounds onto surfaces as diverse as clay, polystyrene, silica, glass, graphite, and metal oxides such as SnO 2 , ZrO 2 , and TiO 2 . However, to the best of our knowledge, the affinity of PEI has not been fully exploited to extend its role and synthesize stabilized AuNPs with the capability of coating different surfaces .…”

“…Additionally, PEI, as a polymeric amine with a high density of charge, has been used to improve the adhesion of different compounds onto surfaces as diverse as clay, polystyrene, silica, glass, graphite, and metal oxides such as SnO 2 , ZrO 2 , and TiO 2 . However, to the best of our knowledge, the affinity of PEI has not been fully exploited to extend its role and synthesize stabilized AuNPs with the capability of coating different surfaces . Recently, we have reported the one‐pot synthesis of PEI‐coated AuNPs that combines the reductant/stabilizer properties of PEI and the use of use of microwave irradiation to yield DNA delivery nanocarriers .…”

Stationary phases for capillary electrophoresis and capillary electrochromatography

“…A polyethyleneimine (PEI) coating on the inner surface of fused-silica capillaries for CE separation of proteins and peptides is reported by Bedia Erim et al [38]. Four polycationic polymers, including polyethyleneimine 1,5-dimethyl-1,5-diazaundecamethylene polymethobromide (Polybrene), poly(methoxyethoxyethyl)ethyleneimine and poly(diallyldimethylammonium chloride) were examined by Cordova et al [39] as noncovalent coatings for capillaries in CE separation of proteins to limit the adsorption of proteins on the walls of fused-silica capillaries.…”

Electrophoretic separation of environmentally important phenolic compounds using montomorillonite‐coated fused‐silica capillaries

“…Cationic coatings have several advantages such as reducing or neutralizing the m EOF and providing a positively charged surface for anchoring other coatings. Amines such as polyamine [11], poly(ethyleneimine) [12,13], Polybrene [14], and poly(diallyldimethyl ammonium chloride) (PDDA) [15,16] have been used to alter the m EOF and improve separations in CE. PDDA has been also used in combination with anionic polymers, to form polyelectrolyte multilayers on silica capillaries [17].…”