Performance of a New Microfluidic Dengue NS1 Immuno-magnetic Agglutination Assay for the Rapid Diagnosis of Dengue Infection in Adults

Wongsawat, Ekkarat; Suputtamongkol, Yupin; Assanasaen, Susan; Silpasakorn, Saowaluk; Avirutnan, Panisadee; Puttikhunt, Chunya; Angkasekwinai, Nasikarn; Yamasmith, Eakkawit; Prommool, Tanapan; Niwattayakul, Kanigar

doi:10.4269/ajtmh.20-1558

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…1b). In contrast, the SD BIOLINE Dengue NS1 antigen kit was reported to show sensitivities less than 80% when compared with a newly developed serotyping NS1 ELISA system using DENV-2 and DENV-4 patient specimens in Thailand [37,38]. Previous evaluations of both the commercial and newly developed RDT NS1 detection systems showed false-negative results and various levels of sensitivity [17,24,[39][40][41].…”

Section: Discussionmentioning

confidence: 99%

Performance of the nonstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, Thailand

Poltep

Phadungsombat

Kosoltanapiwat

et al. 2022

Virol J

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Background: Dengue is an arboviral disease that has a large effect on public health in subtropical and tropical countries. Rapid and accurate detection of dengue infection is necessary for diagnosis and disease management. We previously developed highly sensitive immunochromatographic devices, the TKK 1st and TKK 2nd kits, based on dengue virus (DENV) nonstructural protein 1 detection. However, these TKK kits were evaluated mainly using DENV type 2 clinical specimens collected in Bangladesh, and further validation using clinical specimens of other serotypes was needed. Methods: In the present study, one of the TKK kits, TKK 2nd, was evaluated using 10 DENV-1, 10 DENV-2, 4 DENV-3, 16 DENV-4, and 10 zika virus-infected clinical specimens collected in Bangkok, Thailand. Results: The TKK 2nd kit successfully detected all four DENV serotypes in patient serum specimens and did not show any cross-reactivities against zika virus serum specimens. The IgM and/or IgG anti-DENV antibodies were detected in seven serum specimens, but did not seem to affect the results of antigen detection in the TKK 2nd kit. Conclusion: The results showed that the TKK 2nd kit successfully detected all four DENV serotypes in clinical specimens and confirmed the potential of the kit for dengue diagnosis in endemic countries.

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Section: Discussionmentioning

confidence: 99%

Performance of the nonstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, Thailand

Poltep

Phadungsombat

Kosoltanapiwat

et al. 2022

Virol J

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“…Data from significant recent evaluation studies [15][16][17][18][19][20][21][22][23], using RT-PCR as reference, are summarized in Table 3. These data confirm the variable performance observed in earlier studies.…”

Section: Discussionmentioning

confidence: 99%

Prospective Evaluation of a Commercial Dengue NS1 Antigen Rapid Diagnostic Test in New Caledonia

Alidjinou

Tardieu²,

Vrenken³

et al. 2022

Microorganisms

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Dengue virus infection is endemic in New Caledonia, with outbreaks occurring every year. We evaluated the Biosynex® Dengue NS1 antigen rapid diagnostic test (RDT) for the early diagnosis of dengue in patients attending a local hospital in northern New Caledonia. Samples collected from patients suspected of dengue infection were tested with RDT at the local laboratory, and then sent to the reference laboratory for confirmation with real-time RT-PCR. A total of 472 samples were included during the study period. RT-PCR yielded a positive result in 154 samples (32.6%). The sensitivity and specificity of the NS1 antigen RDT were 79.9% and 96.2%, respectively. The performance of the RDT varied by the time of sampling and dengue virus serotype. In conclusion, Biosynex® Dengue NS1 antigen RDT showed a sensitivity and a specificity in the upper range usually reported for this type of test. Several factors can lead to a suboptimal sensitivity, and negative samples with suggestive clinical features should be retested with reference methods.

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“…However, the proposed method emerges as an alternative powerful assay for rapid dengue diagnostic testing based on NS1 detection because it provides results within 15 min through a semiquantitative measurement and uses a small volume of sample (30 μL). A comparative study was performed by Wongsawat and colleagues (84), who achieved similar performance of the IMA assay compared to ELISA and better performance compared to immunochromatographic test for early and rapid diagnosis of DENV infections in endemic countries.…”

Section: Viral Diseasesmentioning

confidence: 99%

Low-Cost Microfluidic Systems for Detection of Neglected Tropical Diseases

Pinheiro

Guinati

Moreira

et al. 2023

Annual Rev. Anal. Chem.

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Neglected tropical diseases (NTDs) affect tropical and subtropical countries and are caused by viruses, bacteria, protozoa, and helminths. These kinds of diseases spread quickly due to the tropical climate and limited access to clean water, sanitation, and health care, which make exposed people more vulnerable. NTDs are reported to be difficult and inefficient to diagnose. As mentioned, most NTDs occur in countries that are socially vulnerable, and the lack of resources and access to modern laboratories and equipment intensify the difficulty of diagnosis and treatment, leading to an increase in the mortality rate. Portable and low-cost microfluidic systems have been widely applied for clinical diagnosis, offering a promising alternative that can meet the needs for fast, affordable, and reliable diagnostic tests in developing countries. This review provides a critical overview of microfluidic devices that have been reported in the literature for the detection of the most common NTDs over the past 5 years. Expected final online publication date for the Annual Review of Analytical Chemistry, Volume 16 is June 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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Performance of a New Microfluidic Dengue NS1 Immuno-magnetic Agglutination Assay for the Rapid Diagnosis of Dengue Infection in Adults

Cited by 3 publications

References 23 publications

Performance of the nonstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, Thailand

Performance of the nonstructural 1 Antigen Rapid Test for detecting all four DENV serotypes in clinical specimens from Bangkok, Thailand

Prospective Evaluation of a Commercial Dengue NS1 Antigen Rapid Diagnostic Test in New Caledonia

Low-Cost Microfluidic Systems for Detection of Neglected Tropical Diseases

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