Performance of a Multispectral Optoacoustic Tomography (MSOT) System equipped with 2D vs. 3D Handheld Probes for Potential Clinical Translation

Abstract

HighlightsBoth the 2D and 3D handheld MSOT systems hold promise for clinical translation.The 2D MSOT handheld probe provides high resolution, sensitivity, accuracy and reliability.The 3D MSOT handheld probe adds unique 3D capabilities and shortens imaging time.

Lymph Node Micrometastases and In-Transit Metastases from Melanoma: In Vivo Detection with Multispectral Optoacoustic Imaging in a Mouse Model

“…melanin expression driven by a vaccinia virus in lymph nodes (19) as well as melanin as a correlate of melanoma growth in brain metastases-bearing mice (20). In the recent first clinical feasibility study in 20 patients, MSOT was not only able to reliably depict SLN with use of indocyanine green and help guide SLN excision, but also could provide specific information about the node status with high sensitivity (18).…”

“…Neuschmelting et al (20). In brief, the 3D handheld MSOT probe consists of a spherical array of 384 ultrasonic detector elements and allows a tomographic coverage angle of 120° with a center frequency of 4 MHz, resulting in an optimal resolution of 240 mm reached at 65 mm around the center of rotation in the center of the field of view (10 3 10 3 15 mm).…”

“…The illumination beam was, thus, generated by the same wavelength-tunable optical parametric oscillator and the same presets (five frames per wavelength, seven wavelengths in total) as the 2D static tomographic system described earlier, allowing for a maximum raster scanning speed of approximately 3 mm/ sec. However, because the fiber bundles were all fixed in the center of the array, a Pockels cell delay was adjusted to 250 msec to reduce the overall laser energy for the output wavelengths to comply with the skin exposure guidelines for humans as previously reported (20 (22) with the mouse positioned prone on the built-in animal bed after it was fasted for 3 hours. The mice received a retro-orbital injection of 250-300-mCi FDG (IBA Molecular, Reston, Va; total volume = 50-80 mL, 0.11 mCi/mmol, nm.…”

Biomedical photoacoustics: fundamentals, instrumentation and perspectives on nanomedicine

“…Comparing both detectors, the 2D probe performs better in lowering signal decrease and anatomical resemblance, while the 3D probe is prominent in rapid 3D imaging which enables the visualization of superficial areas. 10 In addition, the Twente Group in collaboration with ESAOTE Europe BV developed a dual-imaging modality by combing a US transducer array and a diode stack laser in a single US probe connected to a commercially available US system. The new modality integrates the advantages of both US and PAI in imaging, with the anatomic details being provided by US and the functional information by PAI.…”

“…MsOt (ithera Medical) 10,15 Photoacoustic tomography real-time, whole-body tomography with body navigations Preclinical whole-body PAI especially cancer detection and brain imaging LOUIs-3D (tomo Wave Laboratories, Inc.) 16 …”