Performance of a Commercial Immunoassay for Detection and Differentiation of Periodontal Marker Bacteria: Analysis of Immunochemical Performance With Clinical Samples

Eggert, F M; McLeod, M. Herbert; Flowerdew, Gordon

doi:10.1902/jop.2000.72.9.1201

Journal of Periodontology

2001

DOI: 10.1902/jop.2000.72.9.1201

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Performance of a Commercial Immunoassay for Detection and Differentiation of Periodontal Marker Bacteria: Analysis of Immunochemical Performance With Clinical Samples

F M Eggert

M. Herbert McLeod

Gordon Flowerdew

Abstract: We ascribe the increased sensitivity of the immunoassay in our study to: 1) a more standardized and vigorous sample dispersion that improves release of particulate and soluble antigens from dental plaque biofilm, and 2) better visualization of the reaction product of the enzyme-linked immunoassay. High-technology assays, such as diagnostic immunoassays, have a significant potential for future development in dental diagnosis, because they simplify detection and measurement of biologically important markers such… Show more

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Cited by 3 publications

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Microbiological diagnostic testing in the treatment of periodontal diseases

Loomer¹

2004

Periodontology 2000

113

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A variety of microbiological diagnostic tests are available for clinicians to use for evaluation of patients with periodontal disease. Each one has its own unique set of advantages and disadvantages, and probably the most useful information for the clinician can be obtained using a combination of the various analytic methods. The tests appear to have their greatest utility when used on patients with chronic or aggressive periodontitis who do not respond favorable to conventional mechanical therapy. The major limitation of all microbiological tests is that the information obtained is relevant to the site sampled, and may not be representative of the microflora of the entire dentition. However, since it is often only specific sites that do not respond to initial therapy, knowing the constituents of the microflora that populate these sites is clinically relevant.

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Microbiological diagnostic testing in the treatment of periodontal diseases

Loomer¹

2004

Periodontology 2000

113

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Targeted Delivery of a Photosensitizer to Aggregatibacter actinomycetemcomitans Biofilm

Suci

Kang

Gmür

et al. 2010

Antimicrob Agents Chemother

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The ability to selectively target specific biofilm species with antimicrobials would enable control over biofilm consortium composition, with medical applications in treatment of infections on mucosal surfaces that are colonized by a mixture of beneficial and pathogenic microorganisms. We functionalized a genetically engineered multimeric protein with both a targeting moiety (biotin) and either a fluorophore or a photosensitizer (SnCe6). Biofilm microcolonies of Aggregatibacter actinomycetemcomitans, a periodontal pathogen, were targeted with the multifunctional dodecamer. Streptavidin was used to couple biotinylated dodecamer to a biotinylated anti-A. actinomycetemcomitans antibody. This modular targeting approach enabled us to increase the loading of photosensitizer onto the cells by a cycle of amplification. Scanning laser confocal microscopy was used to characterize transport of fluorescently tagged dodecamer into the microcolonies and targeting of the cells with biotin-labeled, fluorescently tagged dodecamer. Light-induced activity of the targeted photosensitizer reduced the viability of A. actinomycetemcomitans biofilm, as indicated by membrane permeability to propidium iodide. The functionalized multimeric protein promises to be a useful tool for controlling periodontal biofilm consortia and offers a modular design whereby moieties that target different species can be readily combined with the functionalized protein construct.

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Effects of Smoking and Treatment Status on Periodontal Bacteria: Evidence That Smoking Influences Control of Periodontal Bacteria at the Mucosal Surface of the Gingival Crevice

Eggert

McLeod

Flowerdew

2001

Journal of Periodontology

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Smoking-associated periodontitis is not simply a reflection of oral cleanliness. Smoking extends a favorable habitat for bacteria such as P. gingivalis, P. intermedia, and A. actinomycetemcomitans to shallow sites (< or =5 mm). Molecular byproducts of smoking interfere with mechanisms that normally contain growth of damaging bacteria at the surface of the oral mucosa in gingival crevices. In this way, smoking can promote early development of periodontal lesions.

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Performance of a Commercial Immunoassay for Detection and Differentiation of Periodontal Marker Bacteria: Analysis of Immunochemical Performance With Clinical Samples

Cited by 3 publications

References 43 publications

Microbiological diagnostic testing in the treatment of periodontal diseases

Microbiological diagnostic testing in the treatment of periodontal diseases

Targeted Delivery of a Photosensitizer to Aggregatibacter actinomycetemcomitans Biofilm

Effects of Smoking and Treatment Status on Periodontal Bacteria: Evidence That Smoking Influences Control of Periodontal Bacteria at the Mucosal Surface of the Gingival Crevice

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