Performance, intestinal morphometry, and incubation parameters of broiler chickens submitted to in ovo feeding with different techniques and amino acids

Abstract

The objective of this research was to evaluate techniques, incubation period, and effects of amino acids in ovo feeding. First, 240 hatching eggs were selected and distributed in a 2 × 3 factorial arrangement (two techniques and 3 d). The parameters of incubation, relative weight of gastrointestinal organs, and classification of embryonic mortality were evaluated. In the second stage, 720 hatching eggs were incubated with five treatments: control, methionine (20 and 30 mg), and lysine (20 and 30 mg). These animals were housed during 14 d. The animals were divided with five treatments, nine replicates, and groupings based on sex. Incubation parameters, performance, intestinal morphometry, and relative weight of digestive tract organs were evaluated. The results indicate that the technique using a 45° needle axis passing through the air chamber hinders hatchability. In the organ weighing, there was no difference between the evaluated factors. For intestinal morphometry, there was only a significant difference between the dose factor for the crypt villi and crypt diameter. To conclude, the technique using a 90° angle without passing through the air chamber is safer for the embryos. The inoculation of methionine (20 and 30 mg) obtained data similar to the control group.

Effects of in ovo application of resveratrol on hatchability, hatching time, yolk sac weight and chick performance in quail

“…No significant differences were found among treatments group for yolk sac weight (g, %). There are similar studies showing that the injection of in ovo substances has no effect on chick hatching weight (Abdulqader et al, 2018;Groff-Urayama et al, 2019;Maman et al, 2019). On the other hand, Zamani et al, (2018) reported in ovo carbohydrates injection significantly decreased the yolk sac residue compared with control group (P<0.05).…”

“…There are studies showing that in ovo injection of different substances has positive or negative effects on hatchability. Hatchability were adversely affected by in ovo injection propolis (Aygun 2016), amino acids (Groff-Urayama et al, 2019;Nazem et al, 2019), organic trace minerals (Oliveira et al, 2015), and vitamin D3 (Maman et al, 2019).…”

Adding wheat and rapeseed meal to corn-soy diets affects intestinal morphology and nutrient digestibility in broilers

“…The intestinal villi are specialized structures that are very important for nutrient absorption of nutrients from the small intestine (Li et al, 2017;Groff-Urayama et al, 2019). By increasing the height of the villi, their surface area may become greater which can result in increased nutrient absorption (Marcos et al, 2019).…”

In ovo effect of Soursop (Annona muricata L.) leaf extract on hatching and post-hatch performance of Noiler chickens

“…In ovo technology, which is used commercially for embryonic vaccination ( Araujo et al., 2019 ), is a viable tool for developing a wholesome chick quality and performance solution. Researchers have adopted this technology for the delivery of nutrients, ( Groff-Urayama et al., 2019 ; Hajati et al., 2014 ), antibiotics ( Hadi et al., 2014 ) and other supportive substances including plant extracts to avian embryos during incubation ( Coşkun et al., 2014a , b ; N'nanle et al., 2017 ; Sogunle et al., 2022 ; Oke et al., 2021 ), to improve hatching and growth performance of chickens as well as preventing transmission of diseases from eggs to chicks. Though the results have been promising, there exist some contradictions mainly due to the varying nutrient compositions of the substances, extraction techniques employed and the rate of injection ( N'nanle et al., 2017 ; Sogunle et al., 2022 ; Oke et al., 2021 ), which necessitate the exploration of more materials, especially plant materials to validate the in ovo injection approach.…”