Performance evaluation of the Helena V8 capillary electrophoresis system

Poisson, Jessica; Fedoriw, Yuri; Henderson, Matthew; Hainsworth, Shirley A.; Tucker, Kenneth R.; Uddin, Zia; McCudden, Christopher R.

doi:10.1016/j.clinbiochem.2012.03.018

Cited by 12 publications

(8 citation statements)

References 6 publications

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“…The IgA HLC ratio offered identified monoclonal immunoglobulin production and the involved IgA heavy/light chain analysis provided quantitation in patients in whom SPEP was insensitive, either due to comigration or difficulty in distinguishing bands or migration in the beta region or in between the beta and gamma regions, which is a frequent issue in IgA MM . In the current series, SPEP was performed over a 15‐year period on 3 separate devices.…”

Section: Discussionmentioning

confidence: 97%

“…5 The IgA HLC ratio offered identified monoclonal immunoglobulin production and the involved IgA heavy/ light chain analysis provided quantitation in patients in whom SPEP was insensitive, either due to comigration or difficulty in distinguishing bands or migration in the beta region or in between the beta and gamma regions, which is a frequent issue in IgA MM. 4,5 In the current series, SPEP was performed over a 15-year period on 3 separate devices. To eliminate a time bias (heterogeneity of technique, heterogeneity of data collection, etc), a subgroup analysis was performed among patients diagnosed between 2006 and 2010 and demonstrated a similar improve sensitivity of HLC compared with SPEP (data not shown), suggesting that heavy/light chain analysis had superior sensitivity compared with SPEP, regardless of the electrophoretic method used.…”

Section: Discussionmentioning

confidence: 99%

“…Serum protein electrophoresis (SPEP) with M‐spike densitometry is currently the gold standard in the assessment of patients with multiple myeloma (MM) at the time of diagnosis and during therapy . Applying these criteria is not problematic in patients with immunoglobulin G (IgG) MM; however, in patients with IgA MM (approximately 24% of patients with MM), the resolution of the IgA‐spike on SPEP, the presence of IgA dimers, and the electrophoretic migration position with other serum proteins can make accurate densitometric quantification difficult . In addition, the precision of the assessment is inversely correlated with the amount of M‐spike present and the reliance on dye saturation can lead to gross nonlinearity .…”

Section: Introductionmentioning

confidence: 99%

“…1 Applying these criteria is not problematic in patients with immunoglobulin G (IgG) MM 2 ; however, in patients with IgA MM (approximately 24% of patients with MM), 3 the resolution of the IgA-spike on SPEP, the presence of IgA dimers, and the electrophoretic migration position with other serum proteins can make accurate densitometric quantification difficult. 4,5 In addition, the precision of the assessment is inversely correlated with the amount of M-spike present 6 and the reliance on dye saturation can lead to gross nonlinearity. 7 Improved sensitivity is achieved by immunofixation electrophoresis (IFE), but it is a qualitative rather than quantitative assay.…”

Section: Introductionmentioning

confidence: 99%

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IgA kappa/IgA lambda heavy/light chain assessment in the management of patients with IgA myeloma

Boyle

Fouquet

Guidez

et al. 2014

Cancer

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BACKGROUND: Accurate quantification of immunoglobulin A (IgA) monoclonal immunoglobulins by serum protein electrophoresis (SPEP) can be difficult and can impact the assessment of response among patients with multiple myeloma (MM). Therefore, there is a need to identify new assays that better reflect disease burden and response to treatment, and correlate with patient outcome. IgA Hevylite (HLC) measures IgA kappa and IgA lambda separately and provides precise quantitative measurements of the monoclonal IgA expression and polyclonal-isotype matched suppression. In the current study, the authors assessed the usefulness of these assays in the diagnosis of IgA MM and sought to comment on the prognostic value of the assays. METHODS: A study of 157 patients with IgA MM for whom diagnostic samples were available was performed. HLC measurements were performed on a nephelometer and the results were compared with those of electrophoresis. RESULTS: All presentation sera (100 IgA kappa specimens and 57 IgA lambda specimens) were found to have abnormal IgA HLC ratios (IgA kappa median ratio: 336. ). Of the total of 157 patients, 12 patients (8%) presented with oligosecretory myeloma (<10 g/L; including 4 patients with nonquantifiable SPEP bands). HLC uniquely allows for the measurement of isotype paired suppression, which was found to be associated with shortened overall survival in the current study. CONCLUSIONS: In the current study, IgA HLC ratios were found to be abnormal in all patients and the assay was able to produce quantifiable results in more MM sera than either SPEP or total IgA, potentially representing a solution to the issue of comigration and oligosecretory MM. These preliminary data require confirmation in larger prospective trials to validate the usefulness of IgA HLC. Cancer 2014;120:3952-7.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

IgA kappa/IgA lambda heavy/light chain assessment in the management of patients with IgA myeloma

Boyle

Fouquet

Guidez

et al. 2014

Cancer

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“…The Helena V8 is a clinical CE instrument (Helena Biosciences Europe, Newcastle, UK), which is equipped with a monochromator and therefore allows to measure absorbance at any wavelength in the range between 200 and 600 nm . As the interfering substances show UV absorbance spectra that are distinct from those of proteins (Table ), analyzing serum protein pherograms at different wavelengths could allow detection of interfering agents and facilitate pherogram interpretation.…”

Section: Introductionmentioning

confidence: 99%

Dual‐wavelength recording, a simple algorithm to eliminate interferences due to UV‐absorbing substances in capillary electrophoresis

et al. 2014

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Analytical interferences have been described due to the presence of various exogenous UV-absorbing substances in serum. Iodine-based X-ray contrast agents and various antibiotics have been reported to interfere with interpretation of serum protein pherograms, resulting in false diagnosis of paraproteinemia. In the present study, we have explored the possibility of measuring UV absorbance at two distinct wavelengths (210 and 246 nm) to distinguish between true and false paraproteins on a Helena V8 clinical electrophoresis instrument. This study demonstrates that most substances potentially interfering with serum protein electrophoresis show UV-absorption spectra that are distinct from those of serum proteins. Scanning at 246 nm allows detection of all described interfering agents. Comparing pherograms recorded at both wavelengths (210 and 246 nm) enables to distinguish paraproteins from UV-absorbing substances. In case of a true paraprotein, the peak with an electrophoretic mobility in the gamma-region decreases, whereas the X-ray contrast media and antibiotics show an increased absorption when compared to the basic setting (210 nm). The finding of iodine-containing contrast media interfering with serum protein electrophoresis is not uncommon. In a clinical series, interference induced by contrast media was reported in 54 cases (of 13 237 analyses), corresponding with a prevalence of 0.4%. In the same series, 1631 true paraproteins (12.3%) were detected. Implementation of the proposed algorithm may significantly improve the interpretation of routine electrophoresis results. However, attention should still be paid to possible interference due to presence of atypical proteins fractions (e.g., tumor markers, C3).

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Applied capillary electrophoresis system affects screening for monoclonal gammopathy in serum: verification study of two eight-capillary systems

Šegulja

Šparakl

Rogić

2022

Scandinavian Journal of Clinical and Laboratory Investigation

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Performance evaluation of the Helena V8 capillary electrophoresis system

Cited by 12 publications

References 6 publications

IgA kappa/IgA lambda heavy/light chain assessment in the management of patients with IgA myeloma

IgA kappa/IgA lambda heavy/light chain assessment in the management of patients with IgA myeloma

Dual‐wavelength recording, a simple algorithm to eliminate interferences due to UV‐absorbing substances in capillary electrophoresis

Applied capillary electrophoresis system affects screening for monoclonal gammopathy in serum: verification study of two eight-capillary systems

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