Present study was carried out with the aim to determine the effect of genetic and non-genetic factors on milk production efficiency traits and their genetic control. Data on milk production efficiency traits i.e. total milk yield per day of lactation length and total milk yield per day of calving interval in Jersey crossbred animals maintained at organized herd of ICARNational Dairy Research Institute, Eastern Regional Station, Kalyani, Nadia, West Bengal spread over 29 years were analyzed in the study. The overall least-squares means of total milk yield per day of lactation length and total milk yield per day of calving interval were estimated as 6.97±0.21 and 6.02±0.23 kg/day, respectively. The data was grouped into different sub-classes of season of calving, period of calving, parity and genetic group of animals. The random effect of sire had shown significant (p<0.01) influence on milk production efficiency traits. Various environment factors significantly affected the milk production efficiency traits indicating the scope of improvement in management of these crossbred animals. The heritability estimates of 0.54 for TMY/LL and 0.63 for TMY/CI were high in magnitude which suggests that fast genetic progress can be made, if these animals are selected based on the milk production efficiency traits.