Performance evaluation of five commercial assays in assessing seroprevalence of HEV antibodies among blood donors

Al-Absi, Enas S.; Al-Sadeq, Duaa W.; Younis, Manaf H; Yassine, Hadi M.; Abdalla, Omnya M.; Mesleh, Areej G.; Hadwan, Tameem; Amimo, Joshua O.; Thalib, Lukman; Nasrallah, Gheyath K.

doi:10.1099/jmm.0.000807

Cited by 20 publications

(18 citation statements)

References 34 publications

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“…Additionally, the overall positivity rate to the regional countries showed similar rate to Saudi Arabia, but higher than the United Emirates and less than Pakistan [38,39]. Finally, it is essential to highlight the fact that the variance in the prevalence of the transfusion transmissible infections among blood donors from different countries can be a reflection of an actual variance of the prevalence in these countries, in addition to the different methods used to test for these infections which can result in different estimations [40,41].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and trends of transfusion transmissible infections among blood donors in the State of Qatar, 2013–2017

et al. 2020

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Background: Millions of lives around the world are being saved annually through blood transfusion. However, blood transfusion is among the essential vehicles for transmitting infections. The overall prevalence of Transfusion Transmissible Infections among blood donors differs around the world, reflecting the variation in the prevalence of these infections. This study aims to assess the prevalence and trends of Transfusion Transmissible Infections among blood donors in Qatar. Methods: This is a cross-sectional study utilizing donation records of 5 years from January 2013 to December 2017. We included in the study results for all screening and confirmatory tests for Hepatitis B Virus, Hepatitis C Virus, Human T-lymphotropic Virus-I/II, Syphilis and Malaria. Results: Among the 190,509 donations received at the donation centre during the study period, about 91% of donations were received from males and 9% from females. The overall positivity rate for all tests was 1.87, 2.23, 1.78, 2.31, 2.67% for the years 2013 through 2017, with an increasing yearly trend by 6% each year.

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Section: Discussionmentioning

confidence: 99%

Prevalence and trends of transfusion transmissible infections among blood donors in the State of Qatar, 2013–2017

et al. 2020

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“…Low sensitivity of the older version of the Euroimmun assay in comparison to some other assays (including the highly-sensitive and partially-validated Wantai assay) was also shown by others [3][4][5].…”

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confidence: 60%

Underestimation of hepatitis E virus seroprevalence in soldiers from the Polish Special Forces

Bura

Bura²,

Mozer‐Lisewska

2018

International Maritime Health

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“…Since, the study groups are blood donors in both studies, these conflicting results in anti‐HEV seropositivity might be explained by the low performance of the commercial ELISA assay (Euroimmun, Germany) used in the study. In this regard, two recent studies comparing assay performances of Euroimmun and Wantai ELISA assays reported a low assay performance of the Euroimmun assay compared with the Wantai ELISA . In Kayseri anti‐HEV seropositivity was determined as 8.2% and 7.4% with Wantai and Dia.Pro assays in the present study, respectively, although it was previously reported to be 1.6% in blood donors…”

Section: Discussionmentioning

confidence: 98%

HEV seroprevalence in blood donors in Turkey by two commercial total anti‐HEV Ab ELISA kits

Yaşar

Karataylı

Cengiz

et al. 2019

Journal of Medical Virology

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Previous hepatitis E virus (HEV) seroprevalence studies in Turkey have shown high variabilities, leading to conflicting results. We aimed to re‐evaluate HEV seroprevalence among blood donors in Turkey using the Wantai (Beijing, China) and the Dia.Pro (Milan, Italy) total anti‐HEV antibody (Ab) enzyme‐linked immunosorbent assay (ELISA) kits and compare their performances and to investigate the presence of HEV RNA in blood donors. Serum total anti‐HEV antibodies were determined in a total of 2011 volunteer blood donor samples collected from different regions of Turkey (807 from Ankara, 243 from Kayseri, 284 from İzmir, 200 from Malatya, 200 from Kahramanmaraş, and 277 from Van). HEV RNA was evaluated by a real‐time polymerase chain reaction in a total of 272 anti‐HEV seropositive samples. The country‐wide HEV seroprevalence was calculated as 11.5% (Dia.Pro) and 12.2% (Wantai) with seropositivity rates of 12.0%‐12.5% in Ankara, 7.4%‐8.2% in Kayseri, 14.5%‐15.5% in Malatya, 8.1%‐8.8% in İzmir, 15.0%‐16.0% in Kahramanmaraş, and 12.6%‐13.4% in Van by Dia.Pro and Wantai kits, respectively. The lowest detectable Ab concentrations were 0.16 and 0.14 units/mL WHO, for the Dia.Pro and the Wantai assays, respectively, showing no significant difference between assays. HEV RNA was not detected in any of the anti‐HEV seropositive samples. Compared with previous studies, HEV was shown to have a higher overall seroprevalence in Turkey. Despite its limitation, the current study represents the most comprehensive HEV seroprevalence study in Turkey performed with two different commercial ELISA assays with high sensitivities so far. Further investigation is required to determine HEV genotypes in Turkey.

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Performance evaluation of five commercial assays in assessing seroprevalence of HEV antibodies among blood donors

Abstract: Due to discrepancies in the performance of various IgG and IgM assays, seroprevalence studies should be based on furher confirmatory testing for decisive conclusions to be reached.

Cited by 20 publications

References 34 publications

Prevalence and trends of transfusion transmissible infections among blood donors in the State of Qatar, 2013–2017

Prevalence and trends of transfusion transmissible infections among blood donors in the State of Qatar, 2013–2017

Underestimation of hepatitis E virus seroprevalence in soldiers from the Polish Special Forces

HEV seroprevalence in blood donors in Turkey by two commercial total anti‐HEV Ab ELISA kits

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