Performance evaluation of detecting circulating tumor cells and tumor cells in bronchoalveolar lavage fluid in diagnosis of peripheral lung cancer

Zhong, Chang-Hao; Tong, Da; Zhou, Ziqing; Su, Zhu‐Quan; Luo, Yu-Long; Xing, Jia; Bai, Yang; Guo, Su Jie; Li, Shiyue

doi:10.21037/jtd.2017.12.125

Cited by 15 publications

(23 citation statements)

References 18 publications

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“…In a subsequent study, Hur et al showed that BALF EV-based EGFR genotyping has an even better mutation detection rate than tissue/cytology-based typing and that its sensitivity increases with tumor stage, enabling researchers to follow the disease progression and potentially the response to treatment without burdensome repeat biopsies [ 107 ]. Lastly, an opportunity for the identification of lung cancer by BALF-derived liquid biopsy of cfDNA and tumor cells emerged [ 108 , 109 ]. Although research on the clinical usefulness of different components of a liquid biopsy from BALF is only just developing and is nowhere as advanced as similar research concerning blood, it is for sure reasonable and promising, since BALF enables the gathering of both cellular (CTCs) and noncellular components (cfDNA, EVs) directly from the tumor microenvironment, and it provides increased diagnostic sensitivity when compared to blood.…”

Section: Balf-evs As Biomarkersmentioning

confidence: 99%

EVs from BALF—Mediators of Inflammation and Potential Biomarkers in Lung Diseases

Zaręba

Szymański

Homoncik

et al. 2021

IJMS

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Extracellular vesicles (EVs) have been identified as key messengers of intracellular communication in health and disease, including the lung. EVs that can be found in bronchoalveolar lavage fluid (BALF) are released by multiple cells of the airways including bronchial epithelial cells, endothelial cells, alveolar macrophages, and other immune cells, and they have been shown to mediate proinflammatory signals in many inflammatory lung diseases. They transfer complex molecular cargo, including proteins, cytokines, lipids, and nucleic acids such as microRNA, between structural cells such as pulmonary epithelial cells and innate immune cells such as alveolar macrophages, shaping mutually their functions and affecting the alveolar microenvironment homeostasis. Here, we discuss this distinct molecular cargo of BALF-EVs in the context of inducing and propagating inflammatory responses in particular acute and chronic lung disorders. We present different identified cellular interactions in the inflammatory lung via EVs and their role in lung pathogenesis. We also summarize the latest studies on the potential use of BALF-EVs as diagnostic and prognostic biomarkers of lung diseases, especially of lung cancer.

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Section: Balf-evs As Biomarkersmentioning

confidence: 99%

EVs from BALF—Mediators of Inflammation and Potential Biomarkers in Lung Diseases

Zaręba

Szymański

Homoncik

et al. 2021

IJMS

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“…The cut-off value of a CTC positive result was determined as CTC count ≥2, which has been published previously. 11 The upper limits of normality were 35 U/mL for CA125, 5 ng/mL for CEA, and 16.3 ng/mL for NSE.…”

Section: Methodsmentioning

confidence: 90%

“…7,8 This unique CEP8 + CTCs has been examined in a series of studies, which showed a significant association with diagnosis and/or prognosis of lung cancer with promising sensitivity and specificity. [9][10][11] According to the potential application of the CEP8 + CTCs on lung cancer management, this technique was utilized in this study.…”

Section: Introductionmentioning

confidence: 99%

Clinical Utility of Circulating Tumor Cells in the Early Detection of Lung Cancer in Patients with a Solitary Pulmonary Nodule

Zhong

Zhang

Pan

et al. 2021

Technol Cancer Res Treat

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Objective: Lung cancer is the most common cancer and can appear as a solitary pulmonary nodule. Early detection of lung cancer in this patient population would be beneficial for the disease management. In this study, the potential application of circulating tumor cells (CTCs) on early detection of lung cancer in this population was investigated. Methods: The number of CTCs in bronchoalveolar lavage fluid and serum levels of tumor-related markers, cancer antigen 125 (CA125), carcinoembryonic antigen (CEA) and neuron-specific enolase (NSE) were measured in patients with a solitary pulmonary nodule. The association between CTCs and lung cancer was examined. The diagnosis performances of CTCs and selected tumor-related markers were compared. Results: The CTC positivity was significantly associated with lung cancer ( P = .009). The sensitivity of CTCs and CA125, CEA, NSE, and CA125/CEA/NSE was 75%, 5.6%, 0%, 25%, and 33%, respectively. The sensitivity of CTCs was improved from 75% to 83% by the combination with CA125 or NSE. Conclusion: CTCs may be helpful for the early detection of lung cancer in patients with a solitary pulmonary nodule.

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“…The remaining 1283 individuals belonged to the control groups, comprising healthy volunteers in 15 studies 13,[16][17][18][19]21,[23][24][25]27,28,[30][31][32][33] and patients with benign lung disease in 11 studies. 16,18,19,22,27,29,30,[32][33][34][35] Zhou et al 35 and Chen et al 18 included two cohorts and reported the sensitivities and specificities separately, referred to as Zhou QJ1 and Zhou QJ2, and Chen X1 and Chen X2, respectively ( Table 1). The characteristics of the included studies are displayed in Table 1.…”

Section: Search Resultsmentioning

confidence: 99%

“…Among the 21 trials published from 2009 to 2020, 17 studies enrolled patients from East Asian countries/areas (one from Japan and the remaining 16 from People's Republic of China), two studies were performed in the USA, one in Italy and one in France. Immunohistochemistry and immunofluorescence staining were used in 13 studies 13,19,21,22,[24][25][26][27][28][29]31,33,34 and polymerase chain reaction (PCR) identification methods were used in eight studies. [16][17][18]23,30,32,35,36 The count data for primary studies, including true positive (TP), false positive (FP), false negative (FN), and true negative (TN) results were extracted and presented in Table 1.…”

Section: Search Resultsmentioning

confidence: 99%

Role of circulating tumor cells in diagnosis of lung cancer: a systematic review and meta-analysis

et al. 2021

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Objective We systematically reviewed the literature relating to the diagnostic accuracy of circulating tumor cells (CTCs) for the clinical determination of lung cancer. Methods This meta-analysis aimed to evaluate the diagnostic accuracy of CTCs for the clinical determination of lung cancer. The PubMed, Embase, Cochrane Library, and Web of Science databases were searched for relevant studies up to 31 May 2020. The numbers of patients with true positive, false positive, false negative, and true negative results were extracted from each individual study. Pooled sensitivity, specificity, and area under the curve values were calculated with 95% confidence intervals (CI). Results Twenty-one studies with 3997 subjects met the inclusion criteria. The overall diagnostic accuracy was assessed. The pooled sensitivity and specificity were 0.72 (95%CI: 0.65–0.79) and 0.96 (95%CI: 0.91–0.98), respectively, and the pooled positive and negative likelihood ratios were 16.86 (95%CI: 7.65–37.12) and 0.29 (95%CI: 0.23–0.37), respectively. The combined diagnostic odds ratio was 58.12 (95%CI: 24.82–136.09). Conclusion This meta-analysis indicated that CTCs had good diagnostic value for detecting lung cancer.

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Performance evaluation of detecting circulating tumor cells and tumor cells in bronchoalveolar lavage fluid in diagnosis of peripheral lung cancer

Abstract: Detecting tumor cells in peripheral blood and BALF may sensitive to identify benign and malignant peripheral lung lesions and be of value for early diagnosis of lung cancer.

Cited by 15 publications

References 18 publications

EVs from BALF—Mediators of Inflammation and Potential Biomarkers in Lung Diseases

EVs from BALF—Mediators of Inflammation and Potential Biomarkers in Lung Diseases

Clinical Utility of Circulating Tumor Cells in the Early Detection of Lung Cancer in Patients with a Solitary Pulmonary Nodule

Role of circulating tumor cells in diagnosis of lung cancer: a systematic review and meta-analysis

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