Performance Evaluation of Bio Concrete by Cluster and Regression Analysis for Environment Protection

Abstract

The focus of this research is to isolating and identifying bacteria that produce calcite precipitate, as well as determining whether or not these bacteria are suitable for incorporation into concrete in order to enhance the material’s strength and make the environment protection better. In order to survive the high “potential of hydrogen” of concrete, microbes that are going to be added to concrete need to be able to withstand alkali, and they also need to be able to develop endospores so that they can survive the mechanical forces that are going to be put on the concrete while it is being mixed. In order to precipitate CaCO3 in the form of calcite, they need to have a strong urease activity. Both Bacillus sphaericus and the Streptococcus aureus bacterial strains were evaluated for their ability to precipitate calcium carbonate (CaCO3). These strains were obtained from the Department of Biotechnology at GLA University in Mathura. This research aims to solve the issue of augmenting the tension and compression strengths of concrete by investigating possible solutions for environmentally friendly concrete. The sterile cultures of the microorganisms were mixed with water, which was one of the components of the concrete mixture, along with the nutrients in the appropriate proportions. After that, the blocks were molded, and then pond-cured for 7, 28, 56, 90, 120, 180, 270, and 365 days, respectively, before being evaluated for compressibility and tensile strength. An investigation into the effect that bacteria have on compression strength was carried out, and the outcomes of the tests showed that bacterial concrete specimens exhibited an increase in mechanical strength. When compared to regular concrete, the results showed a maximum increase of 16 percent in compressive strength and a maximum increase of 12 percent in split tensile strength. This study also found that both bacterial concrete containing 106, 107, and 108 cfu/ml concentrations made from Bacillus sphaericus and Streptococcus aureus bacteria gave better results than normal concrete. Both cluster analysis (CA) and regression analysis (RA) were utilized in this research project in order to measure and analyze mechanical strength.

Precision Agriculture

“…Noteworthy research endeavors have extensively explored the use of diverse microorganisms like B. subtilis, BKH4 (alkaliphilic bacterium), B. pseudofirmus, Bacillus cohnii, B. alkalinitrilicus, Diaphorobacter nitroreducens, Bacillus sphaericus, and Streptococcus aureus. These microorganisms have proven effective in repairing cracks that measure less than 0.5 mm, through their targeted and specialized activities (Aguilera, et al 2015;Adzami, et al 2018;Ersan, et al 2015;Jonkers, et al 2010;Jroundi, et al 2014;Khaliq, & Ehsan, 2016;Le Metayer-Levrel, et al 1999;Perito, et al 2014;Sarkar, et al 2019;Shukla, et al 2022;Tiano, et al 1999;Wang, et al 2012).…”

Properties of Bacterial Copper Slag Concrete

“…According to researchers, neither the initial nor final setting time of concrete will be affected by biomineralisation. During the biomineralisation process, organic compounds are frequently converted into inorganic crystals, which are then utilised to heal fissures [29]. Moisture and nutrient deficiencies in cement can shorten the lifespan of bacteria.…”

Effect of Marble Dust Powder on the Properties of Fresh and Hard Concrete

“…In the world of construction, concrete is a material of primary importance. The generation of greenhouse gases is directly impacted by the massive cement production in industries [1], [2]. The ability to produce high-quality cement is adversely affected by shortages of highquality limestone.…”