Performance Evaluation of Automated Impedance and Optical Fluorescence Platelet Counts Compared With International Reference Method in Patients With Thalassemia

Tantanate, Chaicharoen; Khowawisetsut, Ladawan; Pattanapanyasat, Kovit

doi:10.5858/arpa.2016-0222-oa

Cited by 19 publications

(27 citation statements)

References 17 publications

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“…For instance, Tantanate et al used samples from thalassemia patients, but we did not restrict to a specific disease population. Furthermore, Tantanate et al [7] found that optical methods were more consistent with the reference method IRM than impedance methods for counting platelets at normal levels, which was also inconsistent with our results. Our results demonstrated that in normal platelet levels, the consistency of the impedance method was higher than the optical method, whether in a Sysmex XN-9000 instrument or a BC-6000 Plus instrument.…”

Section: Discussioncontrasting

confidence: 99%

“…All evaluated methods had acceptable sensitivity, specificity, and coincidence rates for the diagnosis of thrombocytopenia and thrombocytosis, which was consistent with the findings of Tantanate et al [7]. Thus, all assessed methods can be used for screening and exclusion of patients with thrombocytopenia and thrombocytosis.…”

Section: Discussionsupporting

confidence: 88%

“…Tantanate et al [7] revealed that fluorescence was better than optical and impedance methods for counting platelets for samples with thrombocytosis. However, our results showed that the impedance method was superior to optical and fluorescence methods in the thrombocytosis group.…”

Section: Discussionmentioning

confidence: 99%

“…Advia 120, Cell-Dyn 4000, Mindray BC-6800 and Abbot Diagnostics are platelet counters using the optical counting method that can differentiate blood components according to the type and strength of light signals [4–6]. Another analyzer, the Sysmex XN-3000, is designed to use the method of fluorescent nucleic acid staining to count platelets [7]. The above methods have improved the ability of automatic blood analyzers to identify platelets.…”

Section: Introductionmentioning

confidence: 99%

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Compare the accuracy and precision of Coulter LH780, Mindray BC-6000 Plus, and Sysmex XN-9000 with the international reference flow cytometric method in platelet counting

Sun

Huang

et al. 2019

PLoS ONE

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Objective The aim of this study is to evaluate the performance of different platelet counting methods (optical, impedance, fluorescence and hand counting) applied in different analysers by comparing with the international flow cytometric reference method (IRM). Methods A total of 333 blood samples from different subgroups (168 cases with thrombocytopenia, 136 cases with normal platelet counts and 29 cases with thrombocytosis) were tested. Regarding IRM as the gold standard, we compared the accuracy and precision of different platelet count methods; i.e. LH780 (impedance), BC-6000 Plus (optical (O) and impedance (I)), Sysmex XN-9000 (optical (O), impedance (I), fluorescence (F)), and hand counting. Results Sysmex XN-9000-F (r = 0.988) had the best correlation with IRM for thrombocytopenic samples; BC-6000 Plus-I (r = 0.966) was more relevant to IRM than any other method for samples with normal platelet counts. Correlation between Sysmex XN-9000-I (r = 0.960) and IRM was the highest among these methods for samples with thrombocytosis. For bias evaluation, the average bias of Sysmex XN-9000-F was -1.5 × 10 9 /L (95% LA = -9.4 to +6.4) for samples with thrombocytopenia, compared with IRM. BC-6000 Plus-I had a small mean difference with IRM for samples with normal platelet counts or thrombocytosis. Moreover, all evaluated methods had acceptable sensitivity, specificity, and concordance rates as compared with IRM in the diagnosis of thrombocytopenia and thrombocytosis. Conclusions Platelet counting by Sysmex XN-9000-F is more accurate than other methods for thrombocytopenic samples. BC-6000 Plus-I has superior association and consistency for normal platelet counts. As for thrombocytosis patients, Sysmex XN-9000-I has the highest correlation with IRM while Sysmex XN-9000-O has the highest diagnosis efficacy.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Compare the accuracy and precision of Coulter LH780, Mindray BC-6000 Plus, and Sysmex XN-9000 with the international reference flow cytometric method in platelet counting

Sun

Huang

et al. 2019

PLoS ONE

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“…Now the impedance platelet count is widely used in many clinical laboratories for routine patient testing [13][14][15][16]. However, the use of both impedance and optical This study confirmed that PLT-F on XN-9000 is a preferred method for low platelet counting especially the hemolyzed samples.…”

Section: The Qc Results Of Plt-i and Plt-f System At Low Platelet Coumentioning

confidence: 51%

Maintenance of Platelet Counts with Low Level QC Materials and the Change in P-LCR according to Hemolysis with XN-9000

Shim

Lee

2018

Korean J Clin Lab Sci.

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Alinity hq platelet count is not impacted by severe microcytosis

Lakos

Mukhtar

Masi

et al. 2022

Clinical Laboratory Analysis

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Impedance technology has previously been demonstrated to overestimate platelet (PLT) count in samples with microcytosis. [1][2][3][4][5] The most frequent causes of microcytosis are iron deficiency, thalassemia syndromes, and anemia of chronic disease. Tantanate et al 3 . demonstrated that the impedance PLT count by Sysmex XN (PLT-I) showed poor correlation with the International Reference method (IRM), due to high-positive bias and low specificity for the diagnosis of thrombocytopenia, while the optical and fluoresce PLT counts were comparable to the IRM in patients with thalassemia. Several other studies have also concluded that optical or fluorescence-optical

show abstract

Performance Evaluation of Automated Impedance and Optical Fluorescence Platelet Counts Compared With International Reference Method in Patients With Thalassemia

Cited by 19 publications

References 17 publications

Compare the accuracy and precision of Coulter LH780, Mindray BC-6000 Plus, and Sysmex XN-9000 with the international reference flow cytometric method in platelet counting

Compare the accuracy and precision of Coulter LH780, Mindray BC-6000 Plus, and Sysmex XN-9000 with the international reference flow cytometric method in platelet counting

Maintenance of Platelet Counts with Low Level QC Materials and the Change in P-LCR according to Hemolysis with XN-9000

Alinity hq platelet count is not impacted by severe microcytosis

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