Performance evaluation of automated BD Phoenix NMIC-500 panel for carbapenemase detection in carbapenem-resistant and carbapenem-susceptible Enterobacterales

Cho, Hanwool; Kim, Jung Ok; Choi, Ji Eun; Lee, Ho‐Won; Heo, Woong; Jong, Young; Yoo, In Young; Park, Yeon-Joon

doi:10.1016/j.mimet.2020.106042

Cited by 8 publications

(11 citation statements)

References 28 publications

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“…[ 6 7 ] Recent performance evaluation studies of BD Phoenix NMIC-500 panel have shown promising results for accurate detection of CPO. [ 8 9 ] Therefore, the findings of this study w. r. t. CPO detection may be considered significant.…”

Section: Discussionmentioning

confidence: 84%

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Comparative Evaluation of Two Automated ID/AST Systems and Mikrolatest Kit in Assessing the In Vitro Colistin Susceptibility of Carbapenem-Resistant Enterobacteriaceae Isolates

Bhatia¹,

Singh²,

Rani³

et al. 2022

Journal of Pharmacy and Bioallied Sciences

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Aims: To generate preliminary data about comparative evaluation of two automated ID/AST systems and Mikrolatest kit in determining in vitro colistin susceptibility of carbapenem-resistant Enterobacteriaceae spp. Materials and methods: Twenty-three carbapenem-resistant Escherichia coli and Klebsiella pneumoniae and two carbapenem-sensitive multidrug-resistant E. coli isolates obtained from various clinical samples of inpatients were included in the study. Species-level identification and antibiotic susceptibility testing (AST) of test isolates was performed using BD phoenix and MicroScan WalkAway 96 Plus automated systems. Identity was reconfirmed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Additional colistin susceptibility testing was performed using Mikrolatest MIC colistin susceptibility testing kit (reference method). Results: Results showed that 16% isolates (27.3% [3/11] K. pneumoniae and 7.1% [1/14] E. coli ) exhibited in vitro colistin resistance by the reference method. While the categorical agreement between BD Phoenix M50 ID/AST system and reference test w. r. t in vitro colistin susceptibility results was 100% and 92.9% for K. pneumoniae & E. coli , respectively, it was much lower between MicroScan WalkAway 96 plus ID/AST system and the latter. Almost perfect agreement (96%; kappa: 0.834) was observed between BD Phoenix M50 system and reference method. Conclusions: The results of this study are preliminary and cannot be generalized. Multicentric studies with large sample sizes should be conducted throughout the country to gain a deeper understanding of the subject under consideration.

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Section: Discussionmentioning

confidence: 84%

“…Although this panel provides advantage in that the carbapenemase test is automated and the results can be obtained within 6 h, the low specificity in CREs needs to be improved. [ 8 ] (4) Small sample size is another limitation.…”

Section: Discussionmentioning

confidence: 99%

Comparative Evaluation of Two Automated ID/AST Systems and Mikrolatest Kit in Assessing the In Vitro Colistin Susceptibility of Carbapenem-Resistant Enterobacteriaceae Isolates

Bhatia¹,

Singh²,

Rani³

et al. 2022

Journal of Pharmacy and Bioallied Sciences

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“…Several traditional methods have been developed to detect these enzymessuch as biochemical tests, growth-based assays, and immunochromatographic assaysbut they cannot classify carbapenemases and are time-consuming (Hrabák et al, 2014;Nordmann and Poirel, 2019;Quale et al, 2006). Furthermore, many commercial methods have been established in recent years to detect the presence of carbapenemase (Cho et al, 2020;Correa-Martínez et al, 2020), of which the NG-Test CARBA 5 assay, which is a lateral flow immunoassay, can specifically detect Enterobacterales producing NDM, KPC, OXA-48like, IMP, and VIM enzymes (Boutal et al, 2018). The overall sensitivity and specificity of this test were found to be 97.31% and 99.75%, respectively (Hopkins et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the mCIMplus method developed by Petit Morgane et al (Petit et al, 2020) and the automated BD Phoenix NMIC-500 panel evaluated by Cho Hanwool et al (Cho et al, 2020) have undergone certain modifications and improvements based on previous methods; however, these methods still have limitations such as long interpretation time and complicated operation. Nordmann et al (Nordmann et al, 2020) developed a simple and reliable NitroSpeed-Carba NP test to rapidly detect and identify carbapenemase-producing Enterobacter strains.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of NitroSpeed-Carba NP test for rapid identification among different classes of carbapenemases in Enterobacterales and Pseudomonas aeruginosa

Wang

Jia

Sun

et al. 2021

International Journal of Infectious Diseases

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This study aimed to evaluate the performance of the NitroSpeed-Carba NP test for detecting carbapenemases in the clinical strains of Enterobacterales and Pseudomonas aeruginosa (P. aeruginosa), and analyze its advantages and limitations. Methods: The antimicrobial susceptibility tests were performed according to the agar dilution method. Using the modified carbapenemase inactivation method (mCIM), polymerase chain reaction (PCR), and sequencing, the production of carbapenemase and the prevalence of genes were studied. The NitroSpeed-Carba NP test was performed to detect different types of carbapenemases in Enterobacterales and P. aeruginosa. The results of PCR and sequencing were used as the gold standard. Results: Among 144 carbapenemase-producing and 54 carbapenemase-negative strains of Enterobacterales and P. aeruginosa, the NitroSpeed-Carba NP test correctly detected 143 of 144 carbapenemase producers and 51 of 54 non-carbapenemase producers. Moreover, the sensitivity and specificity of all tested isolates were 99.31% and 94.44%, respectively (99.28% and 92.86% for Enterobacterales; 100% and 100% for P. aeruginosa). The sensitivity was 100% for class A (56 of 56), 100% for class B (60 of 60), and 100% for class D (27 of 27). Conclusions:The results suggest that NitroSpeed-Carba NP test is a simple and valuable assay that could be used as a rapid and stable detection method to identify the carbapenemases in Enterobacterales and P. aeruginosa strains.

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“…Few previous studies have investigated the performance of the CPO detect test [25][26][27][28][29][30] . These studies are mainly based on smaller collections of locally or regionally collected/sampled CPO-positive strains and just some 30-50 CPO-negative control strains selected on the basis of resistance without carbapenemase activity-which has already been discussed as a limitation in assessment of test specificity.…”

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confidence: 99%

Evaluation of the BD Phoenix CPO detect panel for prediction of Ambler class carbapenemases

Jonas

Reuter

Klassen

et al. 2021

Sci Rep

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Rapid detection of carbapenemases as a cause of resistance is beneficial for infection control and antimicrobial therapy. The BD Phoenix NMIC-502 panel and CPO detect test identifies presence of carbapenemases in Enterobacterales such as Klebsiella pneumoniae and assigns them to Ambler classes. To evaluate the performance of the CPO detect panel, we employed a European collection of 1222 K. pneumoniae including carbapenem non-susceptible and susceptible clinical isolates from 26 countries, for which draft genomes were available after Illumina sequencing and the presence of carbapenemase genes had been identified by ARIBA gene calling. The CPO panel detected 488 out of 494 carbapenemase-encoding isolates as positive and six as negative. One-hundred and two isolates were tested positive for carbapenemase in the absence of any carbapenemase gene. The CPO panel identified 229 out of 230 KPC-positive isolates as carbapenemase producing and classified 62 of these as class A enzyme. Similarly, the CPO panel correctly specified 167 of 182 as class D. Regarding metallo-beta-lactamases, the CPO panel assigned 78 of 90 MBL positive isolates to class B enzymes. The sensitivity of the CPO panel in detecting carbapenemase activity was 99.5%, 97.7% and 98.3% for class A, B and D enzymes, respectively. The sensitivity in assignation to Ambler class A, B and D was 27%, 86% and 91%, respectively. An overall sensitivity of 98.8% and specificity of 86% in unclassified detection of carbapenemases was observed, with frequent false positive detection of carbapenemase producing organisms, thus rendering further confirmatory tests necessary.

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Performance evaluation of automated BD Phoenix NMIC-500 panel for carbapenemase detection in carbapenem-resistant and carbapenem-susceptible Enterobacterales

Cited by 8 publications

References 28 publications

Comparative Evaluation of Two Automated ID/AST Systems and Mikrolatest Kit in Assessing the In Vitro Colistin Susceptibility of Carbapenem-Resistant Enterobacteriaceae Isolates

Comparative Evaluation of Two Automated ID/AST Systems and Mikrolatest Kit in Assessing the In Vitro Colistin Susceptibility of Carbapenem-Resistant Enterobacteriaceae Isolates

Evaluation of NitroSpeed-Carba NP test for rapid identification among different classes of carbapenemases in Enterobacterales and Pseudomonas aeruginosa

Evaluation of the BD Phoenix CPO detect panel for prediction of Ambler class carbapenemases

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